Name: an ultrasonic tool for nerve conduction block in diabetic rat models
Uploaded: 2017-10-20T13:00:00
Description: Watch this Scientific Journal Video about An Ultrasonic Tool for Nerve Conduction Block in Diabetic Rat Models at JoVE.com

这项研究得到了科学技术部 (最多 105-2221-400-001) 和台湾国家卫生研究院 (项目 BN-105-PP-10) 的支持。

台湾国家卫生研究院机构动物保育与使用委员会批准了所有动物协议. 
 1. 糖尿病模型在成年雄性大 (SD) 大鼠中的诱导作用 <ol> <li> 将从笼中取出的大鼠食品颗粒从笼子中移到快速雄性 SD 大鼠 (300-350 g), 用于6小时前的脲嘧啶诱导. </li>
	<li> 制备柠檬酸钠缓冲液 (0.1 米, pH 4.5)。
	<ol> <li> 溶解1.05 克柠檬酸一水合物 (C 6 h 8 o 7 和 #183; h 2 o; 摩?...

<ol>
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对糖尿病大鼠神经病理性神经作用电位的局部和暂时性抑制, 并观察了 HIFU 治疗后的阻滞作用的即刻发生. 为期28天的 CMAPs 随访研究表明, 可以在适当的 HIFU 照射下进行神经传导的安全阻断。因此, HIFU 治疗的上述协议可以为糖尿病大鼠坐骨神经可逆传导阻滞提供一种替代方案。
在这种方法中, 没有神经变性, 感觉神经可以完全恢复在一个小时到几天的轻度神经损伤<sup clas...

口服药物, 针灸1, 和电神经刺激2已被用于治疗痛苦的糖尿病神经病。然而, 口服药物的副作用、针刺的侵入性操作、电神经刺激对治疗效果和患者的依从性都有影响。超声在动物模型中的周围神经阻滞已被调查了几十年3,4,5。对 0.4-1.0 s 6的 10-20 脉冲超声照射后, 大绿蛙的坐骨神经进行了可逆性抑制。阻断神经传导的一个因素是超声7引起的温升。对于神经病的患者, 在低强度超声照射下, 腓神经对复合肌肉动作电位 (CMAPs) 的抑制作用为 2 min8。完全恢复时间在5分钟以内。
最近, 美国食品和药物管理局批准 HIFU 作为非侵入性治疗子宫肌瘤肿瘤9, 疼痛 palliations 骨转移的10, 和前列腺癌11。HIFU 传感器在体外发出声束, 光束在不同的组织介质中传播, 并会聚在靶肿瘤的焦点上。在不损害周围组织的情况下, 立即形成病灶区, 对靶向肿瘤产生局部效应。HIFU 也已被用于抑制神经传导或导致神经失神经在体内实验正常的大 (SD) 大鼠12。此外, 研究了 HIFU 对神经病理性神经的短期和远期影响,13。以往的结果表明, 可逆转或永久块的感觉神经传导可以实现的 HIFU 与适当的参数。除了镇痛应用外, HIFU 还可作为研究神经传导阻滞的重要因素, 以此为基础, 探讨神经病学和疼痛药物的发展。因此, 需要在动物模型中专门针对周围神经的 HIFU 阻断技术平台。本文旨在通过 HIFU 对糖尿病神经病理性大鼠部分或完全阻断外周神经动作电位的方法进行研究。建立糖尿病大鼠模型, 评价周围神经病理性症状。本文介绍了一种专门用于治疗大鼠坐骨神经的 HIFU 平台和实验过程。

<table border="0" cellpadding="0" cellspacing="0" width="965">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:365px;">streptozotocin</td>
			<td style="width:315px;">Sigma</td>
			<td align="right" style="width:119px;">85882</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">citric acid monohydrate&#160;</td>
			<td style="width:315px;">Sigma</td>
			<td style="width:119px;">C1909</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">trisodium citrate dihydrate</td>
			<td style="width:315px;">Sigma</td>
			<td style="width:119px;">W302600</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">glucose meters</td>
			<td>Roche Accu-Check Active</td>
			<td>GC</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">electronic von Frey device</td>
			<td>IITC Life Science</td>
			<td>2390</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">hot plate</td>
			<td>IITC Life Science</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Biopac MP36 acquisition system</td>
			<td>Biopac Systems, Inc.</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">HIFU transducer</td>
			<td>Sonic Concepts</td>
			<td>H108</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">function generator</td>
			<td>Agilent</td>
			<td>33250A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">power amplifier</td>
			<td>Electronics &#38; Innovation</td>
			<td>1040L</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:365px;">Rats&#160;</td>
			<td style="width:315px;">Biolasco taiwan</td>
			<td style="width:119px;">Sprague-Dawley</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:365px;">Puralube vet ointment</td>
			<td style="width:315px;">Dechra</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">isoflurane vaporizer</td>
			<td style="width:315px;">Parkland Scientific</td>
			<td style="width:119px;">V3000PS</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:365px;">Isoflurance</td>
			<td style="width:315px;">Attane</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:365px;">Restraint bag (Decapicones)</td>
			<td style="width:315px;">Braintree Scientific</td>
			<td style="width:119px;">DC 200</td>
			<td></td>
		</tr>
	</tbody>
</table>

an ultrasonic tool for nerve conduction block in diabetic rat models

近年来, 在正常和糖尿病动物模型中, 采用高强度聚焦超声 (HIFU) 传感器进行神经传导阻滞。HIFU 在使用适当的超声参数时, 可以在不损伤神经的情况下可逆地阻断周围神经的传导。神经活动电位的暂时和部分阻滞表明, HIFU 有潜力是一个有用的临床治疗疼痛缓解。这项工作演示了用 HIFU 换能器抑制糖尿病大鼠神经病理性神经的动作电位的方法在体内。第一步是通过注射链脲佐菌素生成成年男性糖尿病神经病理性大鼠。第二步是通过电子弗雷探针和热板对糖尿病大鼠周围糖尿病神经病变进行评价。最后一步是记录在体内暴露于 HIFU 超声的神经的胞外动作电位。该方法可为超声镇痛应用研究带来有益的结果。

在体内研究表明, HIFU 剂量为 3 s 超声在强度为2810瓦特/厘米2, CMAPs 被抑制20% 的基线, 但他们完全恢复后, 30 分钟 (图 2A, 钻石) 和几乎在28天 (图 2B, 菱形) 期间的常量。对于 5 s HIFU 暴露在相同强度, CMAPs 下降到 65.4% (9.5%) 的基线在4分钟, 恢复到 73.7% (12.6%) 的基线, 120 分钟 (图 2A, 正方形)。CMAPs 直?...

Watch this Scientific Journal Video about An Ultrasonic Tool for Nerve Conduction Block in Diabetic Rat Models at JoVE.com

An Ultrasonic Tool for Nerve Conduction Block in Diabetic Rat Models

本工作提出了应用高强度聚焦超声阻断糖尿病神经病理性神经动作电位的方法。

糖尿病大鼠模型神经传导阻滞的超声工具

Nerve conduction block with a high intensity-focused ultrasound (HIFU) transducer has been performed in normal and diabetic animal models recently. HIFU ...

Research

JoVE Journal

Bioengineering

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

在振荡压力灌流器程序大鼠肝脏的脱细胞

Decellularization and recellularization of parenchymal organs may enable the generation of functional organs in vitro, and several protocols for rodent ...

科研

生物工程

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

支架支持胰岛移植糖尿病小鼠附睾脂肪垫

Islet transplantation has been clinically proven to be effective at treating type 1 diabetes. However, the current intrahepatic transplantation strategy ...

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

一个<em&gt;体外</em鼠类的椎间盘&gt;器官培养模型

Intervertebral disc (IVD) degeneration is a significant contributor to low back pain. The IVD is a fibrocartilaginous joint that serves to transmit and ...

A Performance-testing Platform for a Conduction Micropump with an FR-4 Copper-clad Electrode Plate

FR-4 铜包电极板导电微泵性能测试平台

Here, a conduction micropump with symmetric planar electrode pairs prepared on flame-retardant glass-reinforced epoxy (FR-4) copper-clad laminate (CCL) is ...

An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur

大鼠牵张成骨的高效可再生性协议

This protocol describes the use of a newly developed external fixator for distraction osteogenesis in a rat femoral model. Distraction osteogenesis (DO) ...

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

单细胞基因组测序的超高通量微流控平台

Sequencing technologies have undergone a paradigm shift from bulk to single-cell resolution in response to an evolving understanding of the role of ...

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

大鼠体内部分肝工程的外科技术基础

Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver ...

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

用于长期无细胞基因表达传导的多层微流体平台

The limitations of cell-based synthetic biology are becoming increasingly apparent as researchers aim to develop larger and more complex synthetic genetic ...

Fabrication of the Composite Regenerative Peripheral Nerve Interface (C-RPNI) in the Adult Rat

Fabrication of the Composite Regenerative Peripheral Nerve Interface C-RPNI in the Adult Rat

成年大鼠复合再生外周神经接口（C-RPNI）的制造

Recent advances in neuroprosthetics have enabled those living with extremity loss to reproduce many functions native to the absent extremity, and this is ...

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

长期纳米材料暴露后体外基因毒性测试的高级 3D 肝脏模型

Due to the rapid development and implementation of a diverse array of engineered nanomaterials (ENM), exposure to ENM is inevitable and the development of ...