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DOI :
10.3791/55687-v
•
9:16 min
April 20th, 2017
Chapters
0:05
Title
8:36
Conclusion
7:38
Results: Secretion and Imaging Using Primary Small Intestinal Cultures
0:54
Tissue Preparation
3:15
Removal of Cell Debris and Single Cells
4:44
Collection of Crypt Fragments
6:55
Intestinal Culture Plating
这个协议描述混合鼠小肠细胞的分离和培养。这些一次肠培养使底层使用多种技术肠肽分泌信号转导途径的研究。
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