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DOI :
10.3791/55687-v
•
9:16 min
April 20th, 2017
Chapters
0:05
Title
8:36
Conclusion
7:38
Results: Secretion and Imaging Using Primary Small Intestinal Cultures
0:54
Tissue Preparation
3:15
Removal of Cell Debris and Single Cells
4:44
Collection of Crypt Fragments
6:55
Intestinal Culture Plating
이 프로토콜은 혼합 된 쥐의 소장 세포의 분리 및 문화에 대해 설명합니다. 이러한 기본 장 배양 기법들을 사용하여 장내 펩타이드 분비 하부 신호 전달 경로의 조사를 가능하게한다.
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