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DOI :
10.3791/55700-v
August 3rd, 2017
Chapters
0:05
Title
0:59
Preparation of Dissection Instruments and Reagents
10:03
Conclusion
8:32
Results: Typical Whole-cell Curent in Single Epididymal Principal Cells
2:10
Isolation of Epithelial Cells from Rat Cauda Epididymides
6:09
Patch-clamp Experiment
本発明者らは、ラット尾部精巣上体からの一次解離上皮細胞の電気的特性を測定するために、細胞分離および全細胞パッチクランプ記録を組み合わせたプロトコルを提示する。このプロトコルは、副睾丸の生理的役割をさらに解明するために、初代副睾丸上皮細胞の機能的特性の調査を可能にする。
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