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DOI :
10.3791/55709-v
•
8:28 min
December 27th, 2017
Chapters
0:05
Title
1:01
Agarose Embedding and Vibratome Slicing of Human Lung Tumors
3:51
Immunostaining of Resident CD8 T Cells and Tumor Cells
5:07
Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
7:03
Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
7:53
Conclusion
本协议描述了一种图像驻留肿瘤浸润 CD8 T 细胞标记的人肺肿瘤切片荧光耦合抗体的方法。这项技术允许 real-time 分析 CD8 T 细胞迁移使用共聚焦显微镜。
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