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DOI :
10.3791/55709-v
•
8:28 min
December 27th, 2017
Chapters
0:05
Title
1:01
Agarose Embedding and Vibratome Slicing of Human Lung Tumors
3:51
Immunostaining of Resident CD8 T Cells and Tumor Cells
5:07
Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
7:03
Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
7:53
Conclusion
このプロトコルでは、イメージの居住者腫瘍浸潤 CD8 T 細胞ひと肺腫瘍スライス内結合蛍光抗体で標識する方法について説明します。この手法は、共焦点顕微鏡を用いた CD8 T 細胞の移動の実時間解析を許可します。
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