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DOI :
10.3791/55710-v
June 26th, 2017
Chapters
0:05
Title
0:46
Induction of Hypoxia In Vivo
6:42
Conclusion
5:13
Results: The Effects of Hypoxia on HIF-1α and on Cell Proliferation
2:40
Induction of Hypoxia in Embryos and Collection of Tissue to Monitor HIF-1α Levels
4:19
Incubation of Embryos in Hypoxia for EdU Incorporation
我们引进了一种新颖的低氧室系统,用于水生生物,如青蛙和斑马鱼胚胎。我们的系统简单,健壮,经济实惠,可以在体内诱导和维持缺氧,长达48小时。我们提供2种可重复的方法来监测缺氧的有效性。
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