Name: isolation of murine adipose tissue-derived microvascular fragments as vascularization units for tissue engineering
Uploaded: 2017-04-30T13:00:00
Description: Watch this Scientific Journal Video about Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering at JoVE.com

Somos gratos pela excelente assistência técnica de Janine Becker, Caroline Bickelmann e Ruth Nickels. Este estudo foi financiado por uma concessão do Deutsche Forschungsgemeinschaft (DFG - Fundação Alemã de Pesquisa) - LA 2682 / 7-1.

Todos os procedimentos foram realizados de acordo com o Instituto Nacional de diretrizes de saúde para o uso de animais de laboratório e seguindo as orientações institucionais (Landesamt für Soziales, Gesundheit und Verbraucherschutz, Abt. Lebensmittel- und Veterinärwesen, Zentralstelle, Saarbrücken, Alemanha). 1. Preparação de Instrumentos Cirúrgicos <ol><li> Manter prontas as tesouras de dissecação, pinças cirúrgicas, uma tesoura pequena de preparação, uma pinça fina e u...

<ol>
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W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+and+emerging+vascularization+strategies+in+skin+tissue+engineering.">Current and emerging vascularization strategies in skin tissue engineering.</a> Crit Rev Biotechnol. 20, 1-13 (2016).</li><li>Utzinger, U., Baggett, B., Weiss, J. A., Hoying, J. B., Edgar, L. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large-scale+time+series+microscopy+of+neovessel+growth+during+angiogenesis.">Large-scale time series microscopy of neovessel growth during angiogenesis.</a> Angiogenesis. 18 (3), 219-232 (2015).</li><li>Laschke, M. W., Menger, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prevascularization+in+tissue+engineering:+Current+concepts+and+future+directions.">Prevascularization in tissue engineering: Current concepts and future directions.</a> Biotechnol Adv. 34 (2), 112-121 (2016).</li><li>Baiguera, S., Ribatti, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelialization+approaches+for+viable+engineered+tissues.">Endothelialization approaches for viable engineered tissues.</a> Angiogenesis. 16 (1), 1-14 (2013).</li><li>Wagner, R. C., Kreiner, P., Barrnett, R. J., Bitensky, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biochemical+characterization+and+cytochemical+localization+of+a+catecholamine-sensitive+adenylate+cyclase+in+isolated+capillary+endothelium.">Biochemical characterization and cytochemical localization of a catecholamine-sensitive adenylate cyclase in isolated capillary endothelium.</a> Proc Natl Acad Sci U S A. 69 (11), 3175-3179 (1972).</li><li>Wagner, R. C., Matthews, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+isolation+and+culture+of+capillary+endothelium+from+epididymal+fat.">The isolation and culture of capillary endothelium from epididymal fat.</a> Microvasc Res. 10 (3), 286-297 (1975).</li><li>Laschke, M. W., Menger, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+tissue-derived+microvascular+fragments:+natural+vascularization+units+for+regenerative+medicine.">Adipose tissue-derived microvascular fragments: natural vascularization units for regenerative medicine.</a> Trends Biotechnol. 33 (8), 442-448 (2015).</li><li>Laschke, M. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vascularisation+of+porous+scaffolds+is+improved+by+incorporation+of+adipose+tissue-derived+microvascular+fragments.">Vascularisation of porous scaffolds is improved by incorporation of adipose tissue-derived microvascular fragments.</a> Eur Cell Mater. 24, 266-277 (2012).</li><li>Frueh, F. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+tissue-derived+microvascular+fragments+improve+vascularization,+lymphangiogenesis+and+integration+of+dermal+skin+substitutes.">Adipose tissue-derived microvascular fragments improve vascularization, lymphangiogenesis and integration of dermal skin substitutes.</a> J Invest Dermatol. 137 (1), 217-227 (2017).</li><li>McDaniel, J. S., Pilia, M., Ward, C. L., Pollot, B. E., Rathbone, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+and+multilineage+potential+of+cells+derived+from+isolated+microvascular+fragments.">Characterization and multilineage potential of cells derived from isolated microvascular fragments.</a> J Surg Res. 192 (1), 214-222 (2014).</li><li>Nakano, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+autotransplantation+of+microvessel+fragments+on+experimental+random-pattern+flaps+in+the+rat.">Effect of autotransplantation of microvessel fragments on experimental random-pattern flaps in the rat.</a> Eur Surg Res. 30 (3), 149-160 (1998).</li><li>Nakano, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Successful+autotransplantation+of+microvessel+fragments+into+the+rat+heart.">Successful autotransplantation of microvessel fragments into the rat heart.</a> Eur Surg Res. 31 (3), 240-248 (1999).</li><li>Shepherd, B. R., Hoying, J. B., Williams, S. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microvascular+transplantation+after+acute+myocardial+infarction.">Microvascular transplantation after acute myocardial infarction.</a> Tissue Eng. 13 (12), 2871-2879 (2007).</li><li>Pilia, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transplantation+and+perfusion+of+microvascular+fragments+in+a+rodent+model+of+volumetric+muscle+loss+injury.">Transplantation and perfusion of microvascular fragments in a rodent model of volumetric muscle loss injury.</a> Eur Cell Mater. 28, 11-23 (2014).</li><li>Laschke, M. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+tissue-derived+microvascular+fragments+from+aged+donors+exhibit+an+impaired+vascularisation+capacity.">Adipose tissue-derived microvascular fragments from aged donors exhibit an impaired vascularisation capacity.</a> Eur Cell Mater. 28, 287-298 (2015).</li><li>Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T., Nishimune, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term='Green+mice'+as+a+source+of+ubiquitous+green+cells.">'Green mice' as a source of ubiquitous green cells.</a> FEBS Lett. 407 (3), 313-319 (1997).</li><li>Honek, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modulation+of+age-related+insulin+sensitivity+by+VEGF-dependent+vascular+plasticity+in+adipose+tissues.">Modulation of age-related insulin sensitivity by VEGF-dependent vascular plasticity in adipose tissues.</a> Proc Natl Acad Sci U S A. 111 (41), 14906-14911 (2014).</li><li>Cho, C. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Angiogenic+role+of+LYVE-1-positive+macrophages+in+adipose+tissue.">Angiogenic role of LYVE-1-positive macrophages in adipose tissue.</a> Circ Res. 100 (4), e47-e57 (2007).</li><li>Han, S., Sun, H. M., Hwang, K. C., Kim, S. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose-Derived+Stromal+Vascular+Fraction+Cells:+Update+on+Clinical+Utility+and+Efficacy.">Adipose-Derived Stromal Vascular Fraction Cells: Update on Clinical Utility and Efficacy.</a> Crit Rev Eukaryot Gene Expr. 25 (2), 145-152 (2015).</li><li>Chen, Y. 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Neste estudo apresentamos um protocolo bem estabelecido para o isolamento de ad-MVF. Obtenção ad-MVF do tecido adiposo murino é um procedimento simples com alguns passos críticos. Os ratinhos exibem diferentes subcutânea e depósitos de gordura intra-abdominais. Como anteriormente descrito para os ratos, a fonte de gordura mais adequado para o isolamento de ad-MV F são as almofadas de gordura epididimal devido ao seu tamanho, estrutura homogénea e contaminação mínima com os vasos sanguíneos maiores <sup class...

A engenharia de tecidos centra-se na fabricação de tecidos e de órgãos substitutos que manter, aumentar ou restaurar a função de inoperável em homólogos in vivo 1, 2. O destino de construções de engenharia de tecidos depende essencialmente uma vascularização adequada 3. Redes microvasculares dentro dessas construções devem ser hierarquicamente organizado com arteríolas, capilares e vênulas para permitir perfusão sanguínea eficiente após inosculation a vasculatura do destinatário 4. A geração de tais redes é um dos principais desafios em engenharia de tecidos. Para isso, um amplo espectro de estratégias de vascularização experimentais foi introduzido ao longo das últimas duas décadas 5, 6. abordagens angiogénicos estimular o crescimento de microvasos em receptores tiss engenhariaues por meio de modificação estrutural andaime ou físico-químicas, tais como a incorporação de factores de crescimento 7. No entanto, a vascularização de grandes construes tridimensionais, estratégias dependentes da angiogénese são marcadamente limitados por baixas taxas de crescimento de microvasos a desenvolver 8. Em contraste, o conceito de prevascularization tem como objectivo para a geração das redes microvasculares funcionais dentro do tecido constrói antes da sua implantação 9. Prevascularization convencional envolve a co-cultura de células formadoras de vasos, tais como células endoteliais, células mural ou células-tronco 10, dentro de andaimes. Após a formação da rede microvascular, as construções prevascularized pode então ser implantado num defeitos de tecidos. Contudo, tal abordagem prevascularization é difícil de aplicar em um ambiente clínico, porque se baseia no complexo e demorado in vitro &lt;/ em&gt; procedimentos, que são restritos por grandes obstáculos regulatórios 9. Por conseguinte, existe ainda uma necessidade para o desenvolvimento de novas estratégias prevascularization que são mais adequados para uma aplicação clínica ampla. Tal estratégia prevascularization pode ser a aplicação de fragmentos microvasculares derivadas de tecido adiposo (ad-MVF). ad-MVF representam unidades de vascularização potentes que podem ser colhidas em grandes quantidades a partir do tecido adiposo de ratos 11, 12 e 13 ratinhos. Eles consistem de arteríolas, capilares, e segmentos de vasos venulares, que exibem uma morfologia de microvasos fisiológico com um lúmen e estabilizantes células perivasculares 14, 15. Esta característica única permite a implantação imediata de andaimes ad-MVF-semeado em defeitos de tecido sem pré-cultura. Lá, o ad-MVF remontar rapidamente ema redes microvasculares funcionais. Além disso, ad-MVF representam uma rica fonte de células-tronco mesenquimais 16, que pode ainda contribuir para a sua capacidade de regeneração impressionante. Por conseguinte, ad-MV F são cada vez mais utilizado em diferentes campos da engenharia de tecidos 14, 15, 17, 18, 19, 20, 21. O isolamento de ad-MVF foi estabelecida originalmente em ratos 11, 12. Aqui, nós descrevemos um protocolo, que permite o isolamento padronizado de murino ad-MVF de almofadas de gordura epididimal. Isto pode facilitar uma melhor compreensão sobre os mecanismos moleculares subjacentes à função de ad-MVF usando modelos de ratinhos transgénicos.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>1.5-mL conical microcentrifuge tube</td>
			<td>VWR, Kelsterbach, Germany</td>
			<td>700-5239</td>
			<td></td>
		</tr>
		<tr>
			<td>100-&#181;L precision pipette</td>
			<td>Eppendorf, Hamburg, Germany</td>
			<td>4920000059</td>
			<td></td>
		</tr>
		<tr>
			<td>10-mL measuring pipette</td>
			<td>Costar, Corning Inc., New York, USA</td>
			<td>4488</td>
			<td></td>
		</tr>
		<tr>
			<td>14-mL PP tubes</td>
			<td>Greiner bio-one, Frickenhausen, Germany</td>
			<td>187261</td>
			<td></td>
		</tr>
		<tr>
			<td>1-mL precision pipette</td>
			<td>Eppendorf, Hamburg, Germany</td>
			<td>4920000083</td>
			<td></td>
		</tr>
		<tr>
			<td>500-&#181;m filter (pluriStrainer 500 &#181;m)</td>
			<td>HISS Diagnostics, Freiburg, Germany</td>
			<td>43-50500-03</td>
			<td></td>
		</tr>
		<tr>
			<td>50-mL conical centrifuge tube</td>
			<td>Greiner bio-one, Frickenhausen, Germany</td>
			<td>227261</td>
			<td></td>
		</tr>
		<tr>
			<td>50-mL Erlenmeyer flask</td>
			<td>VWR, Kelsterbach, Germany</td>
			<td>214-0211</td>
			<td></td>
		</tr>
		<tr>
			<td>96-well plate</td>
			<td>Greiner bio-one, Frickenhausen, Germany</td>
			<td>65518</td>
			<td></td>
		</tr>
		<tr>
			<td>cell detachment solution (Accutase)</td>
			<td>eBioscience, San Diego, CA USA</td>
			<td>00-4555-56</td>
			<td></td>
		</tr>
		<tr>
			<td>C57BL/6 mice</td>
			<td>Charles River, Cologne, Germany</td>
			<td>027</td>
			<td></td>
		</tr>
		<tr>
			<td>C57BL/6-Tg(CAG-EGFP)1Osb/J mice</td>
			<td>The Jackson Laboratory, Bar Harbor, USA</td>
			<td>003291</td>
			<td></td>
		</tr>
		<tr>
			<td>CD117-FITC</td>
			<td>BD Biosciences, Heidelberg, Germany</td>
			<td>553373</td>
			<td></td>
		</tr>
		<tr>
			<td>CD31-PE</td>
			<td>BD Biosciences, Heidelberg, Germany</td>
			<td>553354</td>
			<td></td>
		</tr>
		<tr>
			<td>Collagenase NB4G&#160;</td>
			<td>Serva Electrophoresis GmbH, Heidelberg, Germany</td>
			<td>17465.02</td>
			<td>Lot tested by manufacturer for enzymatic activity</td>
		</tr>
		<tr>
			<td>Dissection scissors</td>
			<td>Braun Aesculap AG &#38;CoKG, Melsungen, Germany</td>
			<td>BC 601</td>
			<td></td>
		</tr>
		<tr>
			<td>DNA-binding dye (Bisbenzimide H33342)</td>
			<td>Sigma-Aldrich, Taufkirchen, Germany</td>
			<td>B2261</td>
			<td></td>
		</tr>
		<tr>
			<td>Dulbecco&#39;s modified Eagle medium (DMEM)&#160;</td>
			<td>PAN Biotech, Rickenbach, Germany</td>
			<td>P04-03600</td>
			<td></td>
		</tr>
		<tr>
			<td>Fetal calf serum (FCS)</td>
			<td>Biochrom GmbH, Berlin, Germany</td>
			<td>S0615</td>
			<td></td>
		</tr>
		<tr>
			<td>Fine forceps</td>
			<td>S&#38;T AG, Neuhausen, Switzerland</td>
			<td>FRS-15 RM-8</td>
			<td></td>
		</tr>
		<tr>
			<td>Fine scissors</td>
			<td>World Precision Instrumets, Sarasota, FL, USA</td>
			<td>503261</td>
			<td></td>
		</tr>
		<tr>
			<td>Dermal skin substitute (Integra)</td>
			<td>Integra Life Sciences, Sain Priest, France</td>
			<td>62021</td>
			<td></td>
		</tr>
		<tr>
			<td>Ketamine&#160;</td>
			<td>Serumwerk Bernburg AG, Bernburg, Germany</td>
			<td>7005294</td>
			<td></td>
		</tr>
		<tr>
			<td>M-IgG2akAL488&#160;&#160;</td>
			<td>eBioscience, San Diego, CA USA</td>
			<td>53-4724-80</td>
			<td></td>
		</tr>
		<tr>
			<td>Octeniderm (disinfecting solution)</td>
			<td>Sch&#252;lke &#38; Mayer, Norderstedt, Germany</td>
			<td>118211</td>
			<td></td>
		</tr>
		<tr>
			<td>Penicillin/Streptomycin</td>
			<td>Biochrom, Berlin, Germany</td>
			<td>A2213</td>
			<td></td>
		</tr>
		<tr>
			<td>Petri dish</td>
			<td>Greiner bio-one, Frickenhausen, Germany</td>
			<td>664160</td>
			<td></td>
		</tr>
		<tr>
			<td>Phosphate-buffered saline (PBS)</td>
			<td>Lonza Group, Basel, Switzerland</td>
			<td>17-516F</td>
			<td></td>
		</tr>
		<tr>
			<td>pluriStrainer 20-&#181;m (20 &#181;m filter)</td>
			<td>HISS Diagnostics, Freiburg, Germany</td>
			<td>43-50020-03</td>
			<td></td>
		</tr>
		<tr>
			<td>Rat-IgG2akFITC</td>
			<td>BD Biosciences, Heidelberg, Germany</td>
			<td>553988</td>
			<td></td>
		</tr>
		<tr>
			<td>Rat-IgG2akPE</td>
			<td>BD Biosciences, Heidelberg, Germany</td>
			<td>553930</td>
			<td></td>
		</tr>
		<tr>
			<td>Small preparation scissors</td>
			<td>S&#38;T AG, Neuhausen, Switzerland</td>
			<td>SDC-15 R-8S</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical forceps</td>
			<td>Braun Aesculap AG &#38;CoKG, Melsungen, Germany</td>
			<td>BD510R</td>
			<td></td>
		</tr>
		<tr>
			<td>Tape (Heftpflaster Seide) 1.25 cm</td>
			<td>Fink &#38; Walter GmbH, Mechweiler, Germany</td>
			<td>1671801</td>
			<td></td>
		</tr>
		<tr>
			<td>Xylazine&#160;</td>
			<td>Bayer Vital GmbH, Leverkusen, Germany</td>
			<td>1320422</td>
			<td></td>
		</tr>
		<tr>
			<td>&#945;-SMA-AL488</td>
			<td>eBioscience, San Diego, CA USA</td>
			<td>53-9760-82</td>
			<td>Intracellular labeling additionally requires Cytofix/Cytoperm (BD Biosciences, Heidelberg, Germany; #554722)</td>
		</tr>
	</tbody>
</table>

isolation of murine adipose tissue-derived microvascular fragments as vascularization units for tissue engineering

Uma rede microvascular funcional é de importância crucial para a sobrevivência e integração de construções de tecido de engenharia. Para isso, várias estratégias angiogênicos e prevascularization foram estabelecidas. No entanto, a maioria das abordagens com base em células in vitro incluem passos que consomem tempo para a formação de uma rede microvascular. Assim, eles não são adequados para procedimentos de uma etapa intra-operatórios. Adiposos fragmentos microvasculares derivadas de tecido (ad-MVF) representam unidades de vascularização promissores. Eles podem ser facilmente isolados a partir de tecido gordo e exibem uma morfologia de microvasos funcional. Além disso, eles rapidamente remontar em novas redes microvasculares após implantação in vivo. Além disso, ad-MVF foram mostrados para induzir linfangiogênese. Finalmente, eles são uma fonte rica de células-tronco mesenquimais, que pode ainda contribuir para o seu alto potencial de vascularização. Em estudos anteriores que demonstraram a vascularizati notávelna capacidade de ad-MVF em substitutos ósseos e pele de engenharia. No presente estudo, são descritos em um protocolo padronizado para o isolamento enzimático de ad-MVF de tecido adiposo murino.

No presente estudo foi realizada seis procedimentos de isolamento ad-MVF com tecido adiposo a partir de 7- ao macho de tipo selvagem de 12 meses de idade C57BL 6 ratinhos / (peso corporal médio: 35 ± 1 g). A Figura 1 ilustra a colheita das almofadas de gordura epididimal de murino com subsequente isolamento mecânico e enzimática ad-MVF. O tempo necessário para a colheita de gordura foi de 30 minutos e para o isolamento de ad MV F foi de 120 min. No total, o processo...

Watch this Scientific Journal Video about Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering at JoVE.com

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Apresenta-se um protocolo para isolar fragmentos microvasculares derivadas de tecido adiposo que representam unidades de vascularização promissores. Eles podem ser isolados rapidamente, não requerem processamento in vitro e, assim, pode ser utilizado para prevascularization um passo em diferentes campos da engenharia de tecidos.

Isolamento de Murino adiposo derivada de tecido microvascular Fragmentos como Vascularização Unidades para engenharia de tecidos

A functional microvascular network is of pivotal importance for the survival and integration of engineered tissue constructs. For this purpose, several ...

Research

JoVE Journal

Bioengineering

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

PGS elastomérico Andaimes em Engenharia de Tecidos Arterial

Cardiovascular disease is one of the leading cause of mortality in the US and especially, coronary artery disease increases with an aging population and ...

Bioengenharia

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Processamento de pós-produção de Fibras electrospun para Engenharia de Tecidos

Electrospinning is a commonly used and versatile method to produce  scaffolds (often biodegradable) for 3D tissue engineering.1, 2, 3 Many tissues in vivo ...

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Os tecidos músculo esquelético de engenharia a partir de células progenitoras mioblastos murinos e Aplicação da estimulação elétrica

Engineered muscle tissues can be used for several different purposes, which include the production of tissues for use as a disease model in vitro, e.g. to ...

Tissue Engineering of the Intestine in a Murine Model

Engenharia de Tecidos do Intestino num modelo murino

Tissue-engineered small intestine (TESI) has successfully been used to rescue Lewis rats after massive small bowel resection, resulting in return to ...

Capillary Force Lithography for Cardiac Tissue Engineering

Capilar Força Litografia de Engenharia tecido cardíaco

Cardiovascular disease remains the leading cause of death worldwide1. Cardiac tissue engineering holds much promise to deliver groundbreaking medical ...

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engenharia 3D celularizados Colágeno Gel para Vascular Regeneração Tecidual

Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. ...

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Preparação de Thermoresponsive nanoestruturados Surfaces para Engenharia de Tecidos

Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm)-immobilized surfaces for controlling cell adhesion and detachment were fabricated by the ...

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Engenharia de Tecidos por Intrínseca Vascularização numa<em&gt; In Vivo</em&gt; Câmara Engenharia de Tecidos

In reconstructive surgery, there is a clinical need for an alternative to the current methods of autologous reconstruction which are complex, costly and ...

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Uma plataforma microfluídica para a isolação de alta taxa de transferência de célula única e Cultura

Studying the heterogeneity of single cells is crucial for many biological questions, but is technically difficult. Thus, there is a need for a simple, yet ...

Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

Dois métodos para Decellularization de tecidos vegetais para aplicações de engenharia de tecidos

The autologous, synthetic, and animal-derived grafts currently used as scaffolds for tissue replacement have limitations due to low availability, poor ...