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DOI :
10.3791/55736-v
June 3rd, 2017
Chapters
0:05
Title
9:29
Conclusion
7:44
Results: Representative Effects of EV/Exosome Mammary Epithelial Cell Treatment
1:03
Extracellular Vesicle (EV)/Exosome Isolation and Validation and Exosome Density Gradient Purification
3:50
Mammary Epithelial Cell EV/Exosome Treatment and Fat Pad Injection
6:20
Mammary Gland Whole Mount
このプロトコルは、非接着性/間葉性乳房上皮細胞からの細胞外小胞(EVs)/エキソソームの精製、定量化および特徴づけ方法、ならびにこれらを用いて乳腺上皮細胞に乳腺形成能を転移させる方法を記載する。幹様乳腺上皮細胞に由来するEV /エキソソームは、この細胞特性をEV /エキソソームを摂取する細胞に移入することができる。
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