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DOI :
10.3791/55738-v
June 1st, 2017
Chapters
0:05
Title
7:00
Conclusion
5:59
Results: Isolation, Visualization, amd Analysis of Mitochondrial Supercomplexes
0:58
In-gel Assays for Cx-I and Cx-V
3:19
Electroelution
该方案描述了使用天然电泳分离功能性线粒体电子传递链复合物(Cx)IV及其超复合物,以揭示其组装和结构信息。天然凝胶可以进行免疫印迹,凝胶内测定,并通过电洗脱纯化以进一步表征单个复合物。
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