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DOI :
10.3791/55743-v
June 25th, 2017
Chapters
0:05
Title
0:34
Cell Culture Media and Solution Preparation
7:37
Conclusion
6:52
Results: Effective Glucose Uptake Measurement and Analysis of Response to Insulin Stimulation
2:45
Culture of Human Primary Muscle Cells
4:19
Insulin Stimulation and Glucose Uptake
5:30
Cell Lysis
6:06
Determination of Radiolabeled Glucose
在这种方法中,人原代肌细胞在体外培养以获得分化的肌管,并测量葡萄糖摄取率。我们提供了一个详细的方案,使用放射性标记的[ 3 H] 2-脱氧-D-葡萄糖来量化基础和胰岛素刺激状态的速率。
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