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DOI :
10.3791/55745-v
June 6th, 2017
Chapters
0:05
Title
11:07
Conclusion
9:02
Results: Thy-noc and HU Cell Synchronization Protocols Enable Analysis of Gene Expression During Specific Phases of the Cell Cycle
0:41
Thymidine- and Nocodazole-based (Thy-noc) Synchronization and Release
3:34
HU-based Synchronization and Release from G1/S Boundary
5:14
Collecting Samples for FACS Analysis and RNA Extraction
7:00
RNA Extraction for Gene Expression Analysis
我々は、細胞周期の特定の段階に関連する事象を研究するための状況を提供する2つの細胞同期プロトコールを報告する。我々は、このアプローチが、摂動のない細胞周期または細胞周期に影響を及ぼす薬剤への暴露で特定の遺伝子の調節を分析するのに有用であることを示す。
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