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DOI :
10.3791/55747-v
July 23rd, 2017
Chapters
0:00
Title
0:28
Dissection of the Gut Epithelium from R. microplus
1:56
Epithelial Cell Dissociation
3:36
Isolation of Single Epithelial Cells Using a Percoll Gradient
6:47
Cell Surface Biotinylation and the Isolation of Biotinylated Surface Proteins
9:44
Representative Results
11:17
Conclusion
一种基于梯度改性密度离心方法被利用来上皮细胞从扇头蜱microplus肠组织隔离。生物素化表面结合蛋白,并通过链霉抗生物素蛋白磁珠纯化,用于下游应用。
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