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DOI :
10.3791/55747-v
July 23rd, 2017
Chapters
0:00
Title
0:28
Dissection of the Gut Epithelium from R. microplus
1:56
Epithelial Cell Dissociation
3:36
Isolation of Single Epithelial Cells Using a Percoll Gradient
6:47
Cell Surface Biotinylation and the Isolation of Biotinylated Surface Proteins
9:44
Representative Results
11:17
Conclusion
Rhipicephalus microplus腸組織から上皮細胞を単離するために、改変密度遠心分離勾配に基づく方法論を利用した。表面結合タンパク質をビオチン化し、下流の用途に利用するためにストレプトアビジン磁気ビーズで精製した。
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