Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/55754-v
•
14:13 min
January 1st, 2018
Chapters
0:05
Title
1:02
Preparing Reagents, Samples, and Extraction Controls
3:23
Subculturing Yeast and Preparing YES Plates
7:18
Processing YES Plates (Day 2 of the Assay)
9:26
Calculating LacZ Values
10:28
Interpolating Sample Estradiol Equivalents (EEQs) using 4-parameter Logistic Regression
11:30
Results: Yeast Estrogen Screen Quantifies Ligands in Personal Care Products (PCPs)
12:52
Conclusion
本文介绍了一个优化酵母雌激素屏幕的量化配体的个人护理产品 (杆), 结合雌激素受体α (ERα) 和/或β (er)。该方法采用了两种色度基质选择, 一个六天的冷冻孵化, 用于本科课程, 以及数据分析的统计工具。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved