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DOI :
10.3791/55769-v
August 30th, 2017
Chapters
0:05
Title
1:33
Fluorimeter Setup for Upconversion Spectrum Measurement and Microscope Setup
9:40
Conclusion
8:33
Results: Visualize PKA Signal Transduction Activation via NIR Photoactivation
3:29
Characterization of Caged-PKA and Caged PKA/UCNP Constructs and Photolysis Setup
4:55
Cell Sample Preparation and Microinjection of Caged-PKA/UCNP Complexes
7:35
Photoactivation of Caged-PKA/UCNP Using NIR Light in Living Cells Experiment
在这个协议中, 笼状蛋白激酶 a (PKA), 细胞信号转导 bioeffector, 被固定在纳米粒子表面, 微量进入胞, 并激活由 upconverted 紫外线光近红外线 (近红外) 照射, 诱导下游应力纤维解体的胞。
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