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DOI :
10.3791/55769-v
August 30th, 2017
Chapters
0:05
Title
1:33
Fluorimeter Setup for Upconversion Spectrum Measurement and Microscope Setup
9:40
Conclusion
8:33
Results: Visualize PKA Signal Transduction Activation via NIR Photoactivation
3:29
Characterization of Caged-PKA and Caged PKA/UCNP Constructs and Photolysis Setup
4:55
Cell Sample Preparation and Microinjection of Caged-PKA/UCNP Complexes
7:35
Photoactivation of Caged-PKA/UCNP Using NIR Light in Living Cells Experiment
このプロトコルではケージ プロテインキナーゼ A (PKA)、細胞信号伝達 bioeffector ナノ粒子表面に固定化した細胞質に microinjected、アップ コンバートされる紫外線近赤外線 (NIR) 照射からによって活性化を誘導します。細胞質の下流圧力繊維崩壊。
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