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DOI :
10.3791/55775-v
June 15th, 2017
Chapters
0:05
Title
11:15
Conclusion
10:07
Assembly of 3 Chromoprotein Cassetts Using a C-Brick Standard
1:01
Preparation of crRNA
3:00
Construction of C-Brick Parts
6:11
C-Brick Assembly
CRISPR関連タンパク質Cpf1は、特別に設計されたCRISPR RNA(crRNA)によって導かれ、所望の部位で二本鎖DNAを切断し、粘着末端を生成する。この特性に基づいて、DNAアセンブリ標準(C-Brick)が確立され、その使用方法を詳述したプロトコルがここに記載されている。
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