Name: an experimental protocol for assessing the performance of new ultrasound probes based on cmut technology in application to brain imaging
Uploaded: 2017-09-24T13:00:00
Description: Watch this Scientific Journal Video about An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging at JoVE.com

这项工作得到了国家政府和欧洲联盟的部分支持, 通过 ENIAC JU 项目 DeNeCoR 324257 号赠款协议。作者要感谢 Prof. 乔瓦尼 Magenes, Prof. 皮托尔托利和 Dr. Giosuè Caliano 为他们宝贵的支持, 监督和有见地的意见, 使这项工作成为可能。我们也感谢 Prof. 圣埃吉迪奥和他的小组 (BCC 实验室), 连同基金会依 Neurologico c Mondino, 提供了运动跟踪和 mr 仪器, 并为 Germani 先生收购。最后, 我们要感谢 Dr. 尼可莱塔 Caramia, Dr. Dallai 和 Ms. 芭芭拉 Mauti 为他们的宝贵技术支持和 Mr. 沃尔特沃尔皮提供牛脑。

本视频中显示的所有生物样品都是通过标准的食品供应链获得的。这些标本是按照有关机构的道德和安全条例处理的. 
 注意: 图4中的图表 总结了该协议的8主要阶段。阶段1至4涉及初始活动, 将在美国图像采集和处理阶段开始之前进行一次。这些初步阶段如下: 1. 试验装置的初步设计和琼脂幻影 (用于校准程序);2) 制备的 前体 牛脑;3. 获取大脑的 mr 图像;4. 定性?...

<ol>
	<li>Matrone, G., Savoia, A. S., Terenzi, M., Caliano, G., Quaglia, F., Magenes, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Volumetric+CMUT-Based+Ultrasound+Imaging+System+Simulator+With+Integrated+Reception+and+&#181;-Beamforming+Electronics+Models.">A Volumetric CMUT-Based Ultrasound Imaging System Simulator With Integrated Reception and &#181;-Beamforming Electronics Models.</a> IEEE Trans. Ultrason. Ferroelectr. Freq. Control. 61 (5), 792-804 (2014).</li><li>Pappalardo, M., Caliano, G., Savoia, A. S., Caronti, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Micromachined ultrasonic transducers. Piezoelectric and Acoustic Materials for Transducer Applications. , 453-478 (2008).</li><li>Oralkan, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capacitive+micromachined+ultrasonic+transducers:+Next-generation+arrays+for+acoustic+imaging?.">Capacitive micromachined ultrasonic transducers: Next-generation arrays for acoustic imaging?.</a> IEEE Trans. Ultrason. Ferroelectr. Freq. Control. 49 (11), 1596-1610 (2002).</li><li>Savoia, A., Caliano, G., Pappalardo, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+CMUT+probe+for+medical+ultrasonography:+From+microfabrication+to+system+integration.">A CMUT probe for medical ultrasonography: From microfabrication to system integration.</a> IEEE Trans. Ultrason. Ferroelectr. Freq. Control. 59 (6), 1127-1138 (2012).</li><li>Ramalli, A., Boni, E., Savoia, A. S., Tortoli, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Density-tapered+spiral+arrays+for+ultrasound+3-D+imaging.">Density-tapered spiral arrays for ultrasound 3-D imaging.</a> IEEE Trans. Ultrason. Ferroelectr. Freq. Control. 62 (8), 1580-1588 (2015).</li><li>Lazebnik, R. S., Lancaster, T. L., Breen, M. S., Lewin, J. S., Wilson, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Volume+registration+using+needle+paths+and+point+landmarks+for+evaluation+of+interventional+MRI+treatments.">Volume registration using needle paths and point landmarks for evaluation of interventional MRI treatments.</a> IEEE Trans. Med. Imag. 22 (5), 653-660 (2003).</li><li>Dawe, R. J., Bennett, D. A., Schneider, J. A., Vasireddi, S. K., Arfanakis, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postmortem+MRI+of+human+brain+hemispheres:+T2+relaxation+times+during+formaldehyde+fixation.">Postmortem MRI of human brain hemispheres: T2 relaxation times during formaldehyde fixation.</a> Magn. Reson. Med. 61 (4), 810-818 (2009).</li><li>Chen, S. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+anthropomorphic+polyvinyl+alcohol+brain+phantom+based+on+Colin27+for+use+in+multimodal+imaging.">An anthropomorphic polyvinyl alcohol brain phantom based on Colin27 for use in multimodal imaging.</a> Mag. Res. Phys. 39 (1), 554-561 (2012).</li><li>Farrer, A. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+and+evaluation+of+tissue-mimicking+gelatin+phantoms+for+use+with+MRgFUS.">Characterization and evaluation of tissue-mimicking gelatin phantoms for use with MRgFUS.</a> J. Ther. Ultrasound. 3 (9), (2015).</li><li>Choe, A. S., Gao, Y., Li, X., Compton, K. B., Stepniewska, I., Anderson, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Accuracy+of+image+registration+between+MRI+and+light+microscopy+in+the+ex+vivo+brain.">Accuracy of image registration between MRI and light microscopy in the ex vivo brain.</a> Magn. Reson. Imaging. 29 (5), 683-692 (2011).</li><li>Gobbi, D. G., Comeau, R. M., Peters, T. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound+probe+tracking+for+real-time+ultrasound/MRI+overlay+and+visualization+of+brain+shift.">Ultrasound probe tracking for real-time ultrasound/MRI overlay and visualization of brain shift.</a> Int. Conf. Med. Image Comput. Comput. Assist. Interv (MICCAI) n. 920, 927 (1999).</li><li>Ternifi, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound+measurements+of+brain+tissue+pulsatility+correlate+with+the+volume+of+MRI+white-matter+hyperintensity.">Ultrasound measurements of brain tissue pulsatility correlate with the volume of MRI white-matter hyperintensity.</a> J. Cereb. Blood Flow. Metab. 34 (6), 942-944 (2014).</li><li>Unsgaard, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuronavigation+by+Intraoperative+Three-dimensional+Ultrasound:+Initial+Experience+during+Brain+Tumor+Resection.">Neuronavigation by Intraoperative Three-dimensional Ultrasound: Initial Experience during Brain Tumor Resection.</a> Neurosurgery. 50 (4), 804-812 (2002).</li><li>Pfefferbaum, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postmortem+MR+imaging+of+formalin-fixed+human+brain.">Postmortem MR imaging of formalin-fixed human brain.</a> NeuroImage. 21 (4), 1585-1595 (2004).</li><li>Schulz, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+strain+fields+in+human+brain+resulting+from+formalin+fixation.">Three-dimensional strain fields in human brain resulting from formalin fixation.</a> J. Neurosci. Meth. 202 (1), 17-27 (2011).</li><li>Ahrens, J., Geveci, B., Law, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ParaView:+An+End-User+Tool+for+Large+Data+Visualization.">ParaView: An End-User Tool for Large Data Visualization.</a> Visualization Handbook. , (2005).</li><li>Cloutier, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+multimodality+vascular+imaging+phantom+with+fiducial+markers+visible+in+DSA,+CTA,+MRA,+and+ultrasound.">A multimodality vascular imaging phantom with fiducial markers visible in DSA, CTA, MRA, and ultrasound.</a> Med. Phys. 31 (6), 1424-1433 (2004).</li><li>Boni, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+reconfigurable+and+programmable+FPGA-based+system+for+nonstandard+ultrasound+methods.">A reconfigurable and programmable FPGA-based system for nonstandard ultrasound methods.</a> IEEE Trans. Ultrason. Ferroelectr. Freq. Control. 59 (7), 1378-1385 (2012).</li><li>Bagolini, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PECVD+low+stress+silicon+nitride+analysis+and+optimization+for+the+fabrication+of+CMUT+devices.">PECVD low stress silicon nitride analysis and optimization for the fabrication of CMUT devices.</a> J. Micromech. Microeng. 25 (1), (2015).</li><li>Savoia, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Design+and+fabrication+of+a+cMUT+probe+for+ultrasound+imaging+of+fingerprints.">Design and fabrication of a cMUT probe for ultrasound imaging of fingerprints.</a> Proc. IEEE Int. Ultrasonics Symp. , 1877-1880 (2010).</li><li>Fenster, A., Downey, D. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+ultrasound+imaging.">Three-dimensional ultrasound imaging.</a> Annu. Rev. Biomed. Eng. 2, 457-475 (2000).</li><li>Matrone, G., Ramalli, A., Savoia, A. S., Tortoli, P., Magenes, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+Frame-Rate,+High+Resolution+Ultrasound+Imaging+with+Multi-Line+Transmission+and+Filtered-Delay+Multiply+And+Sum+Beamforming.">High Frame-Rate, High Resolution Ultrasound Imaging with Multi-Line Transmission and Filtered-Delay Multiply And Sum Beamforming.</a> IEEE Trans. Med. Imag. 36 (2), 478-486 (2017).</li><li>Matrone, G., Savoia, A. S., Caliano, G., Magenes, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Depth-of-field+enhancement+in+Filtered-Delay+Multiply+and+Sum+beamformed+images+using+Synthetic+Aperture+Focusing.">Depth-of-field enhancement in Filtered-Delay Multiply and Sum beamformed images using Synthetic Aperture Focusing.</a> Ultrasonics. 75, 216-225 (2017).</li><li>Boni, E., Cellai, A., Ramalli, A., Tortoli, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+high+performance+board+for+acquisition+of+64-channel+ultrasound+RF+data.">A high performance board for acquisition of 64-channel ultrasound RF data.</a> Proc. IEEE Int. Ultrasonics Symp. , 2067-2070 (2012).</li><li>Matrone, G., Savoia, A. S., Caliano, G., Magenes, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Delay+Multiply+and+Sum+beamforming+algorithm+in+medical+ultrasound+imaging.">The Delay Multiply and Sum beamforming algorithm in medical ultrasound imaging.</a> IEEE Trans. Med. Imag. 34, 940-949 (2015).</li><li>Savoia, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+lateral+resolution+and+contrast+in+ultrasound+imaging+using+a+sidelobe+masking+technique.">Improved lateral resolution and contrast in ultrasound imaging using a sidelobe masking technique.</a> Proc. IEEE Int. Ultrasonics Symp. , 1682-1685 (2014).</li><li>Gy&#246;ngy, G., Makra, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+validation+of+a+convolution-+based+ultrasound+image+formation+model+using+a+planar+arrangement+of+micrometer-scale+scatterers.">Experimental validation of a convolution- based ultrasound image formation model using a planar arrangement of micrometer-scale scatterers.</a> IEEE Trans. Ultrason. Ferroelectr. Freq. Control. 62 (6), 1211-1219 (2015).</li><li>Shapoori, K., Sadler, J., Wydra, A., Malyarenko, E. V., Sinclair, A. N., Maev, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+Ultrasonic-Adaptive+Beamforming+Method+and+Its+Application+for+Trans-skull+Imaging+of+Certain+Types+of+Head+Injuries;+Part+I:+Transmission+Mode.">An Ultrasonic-Adaptive Beamforming Method and Its Application for Trans-skull Imaging of Certain Types of Head Injuries; Part I: Transmission Mode.</a> IEEE Trans. Biomed. Eng. 62 (5), 1253-1264 (2015).</li><li>Salles, S., Liebgott, H., Basset, O., Cachard, C., Vray, D., Lavarello, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+evaluation+of+spectral-based+quantitative+ultrasound+imaging+using+plane+wave+compounding.">Experimental evaluation of spectral-based quantitative ultrasound imaging using plane wave compounding.</a> IEEE Trans. Ultrason. Ferroelectr. Freq. Control. 61 (11), 1824-1834 (2014).</li><li>Alessandrini, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+New+Technique+for+the+Estimation+of+Cardiac+Motion+in+Echocardiography+Based+on+Transverse+Oscillations:+A+Preliminary+Evaluation+In+Silico+and+a+Feasibility+Demonstration+In+Vivo.">A New Technique for the Estimation of Cardiac Motion in Echocardiography Based on Transverse Oscillations: A Preliminary Evaluation In Silico and a Feasibility Demonstration In Vivo.</a> IEEE Trans. Med. Imag. 33 (5), 1148-1162 (2014).</li><li>Ramalli, A., Basset, O., Cachard, C., Boni, E., Tortoli, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Frequency-domain-based+strain+estimation+and+high-frame-rate+imaging+for+quasi-static+elastography.">Frequency-domain-based strain estimation and high-frame-rate imaging for quasi-static elastography.</a> IEEE Trans. Ultrason. Ferroelectr. Freq. Control. 59 (4), 817-824 (2012).</li><li>Markley, F. L., Cheng, Y., Crassidis, J. L., Oshman, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Averaging+quaternions.">Averaging quaternions.</a> J. Guid. Cont. Dyn. 30 (4), 1193-1197 (2007).</li><li>Dorst, L., Fontijne, D., Mann, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Geometric Algebra for Computer Science. An Object-oriented Approach to Geometry. , (2007).</li><li>Horn, B. K. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Closed-form+solution+of+absolute+orientation+using+unit+quaternions.">Closed-form solution of absolute orientation using unit quaternions.</a> J. Opt. Soc. Am. A. 4 (4), 629-642 (1987).</li></ol>

在文献中介绍了一些与所提出的协议相似或相关的技术。这些技术也基于实际目标的使用, 包括固定的动物或尸体的大脑, 但它们主要是为测试各种类型的数字注册方法。
然而, 此处描述的协议具有特定的目的, 即在开发的早期阶段对美国探测器进行不同的配置测试, 因此, 它满足了收购的可再现性的基本要求, 即在同一生物标本和具有可比的姿势。该协议介绍了上述现有技术的...

<table border="0" cellpadding="0" cellspacing="0" width="925">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">ULA-OP</td>
			<td style="width:167px;">University of Florence</td>
			<td style="width:104px;">N/A</td>
			<td style="width:415px;">Ultrasound imaging research system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">3D imaging piezeoelectric probe</td>
			<td style="width:167px;">Esaote s.p.a.</td>
			<td style="width:104px;">9600195000</td>
			<td>Mechanically-swept 3D ultrasound probe, model BL-433</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Linear-array piezoelectric probe</td>
			<td style="width:167px;">Esaote s.p.a.</td>
			<td style="width:104px;">122001100</td>
			<td>Ultrasound linear array probe, model LA-533</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">CMUT probe</td>
			<td style="width:167px;">University Roma Tre</td>
			<td style="width:104px;">N/A</td>
			<td>Ultrasound linear array probe based on CMUT technology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">MAGNETOM Skyra 3T MR scanner</td>
			<td style="width:167px;">Siemens Healthcare</td>
			<td style="width:104px;">N/A</td>
			<td>MR scanner</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Head coil</td>
			<td style="width:167px;">Siemens Healthcare</td>
			<td style="width:104px;">N/A</td>
			<td>32-channel head coil for MR imaging</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">NDI Polaris Vicra</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">8700335001</td>
			<td>Optical motion tracking system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Pointer tool</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">8700340</td>
			<td>Passive pointer tool with 4 reflecting markers</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Clamp-equipped tool</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">8700399</td>
			<td>Rigid body with 4 reflecting markers and a clamp to be connected to the US probe handle</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Bovine brain</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Brain of an adult bovine, from food suppliers</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Formalin solution</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>10% buffered formalin solution for bovine brain fixation - CAUTION, formalin is a toxic chemical substance and must be handled with care; specific regulations may also apply (see for instance US OSHA Standard 1910.1048 App A)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Plastic container for anatomical parts</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Cilindrical plastic container with lid</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Glass spheres</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>3mm diameter spheres of Flint glass</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Agar</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>30 g, for phantom preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Glycerine</td>
			<td style="width:167px;">AEFFE Farmaceutici</td>
			<td style="width:104px;">A908005248</td>
			<td>100 g, for phantom preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Distilled water</td>
			<td style="width:167px;">Solbat Gaysol</td>
			<td style="width:104px;">8027391000015</td>
			<td>870 g, for phantom preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Beaker</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Beaker used for the diluition of glycerine and agar in distilled water</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Lysoform</td>
			<td style="width:167px;">Lever</td>
			<td style="width:104px;">8000680500014</td>
			<td>A benzalkonium chloride and water solution was used for the agar phantom preservation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Polystyrene mannequin head</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Polyestirene model which was cutted and used to design the configuration of spheres&#39;patterns</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Green tissue marking dye for histology</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Colour used to mark the glass spheres&#39; positions on the bovine brain surface</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Yellow enamel</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Enamel used to colour the glass spheres implanted in the agar phantom</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Water tank</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>50x50x30 cm plastic tank filled degassed water up to a 15cm height&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Mechanical arm</td>
			<td style="width:167px;">Esaote s.p.a.</td>
			<td style="width:104px;">N/A</td>
			<td>Mechanical arm clamped to the water tank border and used to held the probe in fixed positions</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Plate of synthetic resin</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Plate used as a support for the bovine brain positioning in the water tank</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Sewing threads</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Sewing thread segments used to immobilize the brain on the resin plate</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Adhesive tape</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Adhesive tape used to fix the sewing thread extremities onto the resin plate</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Plastic food container</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Sealed food container used for the agar phantom</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Notebook</td>
			<td style="width:167px;">Lenovo</td>
			<td style="width:104px;">Z50-70</td>
			<td>Lenovo&#160; Z50-70, Intel(R) Core i7-4510U @ 2.0 GHz, 8 GB RAM</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Workstation</td>
			<td style="width:167px;">Dell Inc.</td>
			<td style="width:104px;">T5810</td>
			<td>Intel(R) Xeon(R) CPU E3-1240v3 @ 3.40 GHz, 16 GB RAM</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Matlab</td>
			<td style="width:167px;">The MathWorks</td>
			<td style="width:104px;">R2013a</td>
			<td>Software tool, used for space transformation computation and 3D reconstruction from image planes</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Paraview</td>
			<td style="width:167px;">Kitware Inc.</td>
			<td style="width:104px;">v. 4.4.1</td>
			<td>Open-source software for 3D image processing and visualization</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">NDI Toolbox - ToolTracker Utility</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">v. 4.007.007</td>
			<td>Software for marker position visualization and tracking in the NDI Polaris Vicra measurement volume</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">C++ data-logging software</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">v. 4.007.007</td>
			<td>Software for marker position recording on a text log file</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">ULA-OP software&#160;</td>
			<td style="width:167px;">University of Florence</td>
			<td style="width:104px;">N/A</td>
			<td>Software for real-time display and control of the ULA-OP system</td>
		</tr>
	</tbody>
</table>

an experimental protocol for assessing the performance of new ultrasound probes based on cmut technology in application to brain imaging

对成像性能进行早期和可重复性评估的可能性是新超声 (美国) 探头设计和开发过程中的基础。特别是, 一个更现实的分析与应用特定的成像目标可能是非常宝贵的评估美国探针的预期性能在其潜在的临床应用领域。
在这项工作中提出的实验协议是特意设计的, 以提供一个应用特定的评估程序的新开发的美国探针原型基于电容式微机械超声换能器 (CMUT) 技术的相关脑部成像
该协议结合了使用的牛脑固定在福尔马林作为成像靶, 这既确保了现实主义和重复性的描述的程序, 以及神经导航技术借用从外科。美国探针实际上是连接到一个运动跟踪系统, 获取位置数据, 并使美国图像的叠加, 以参考磁共振 (MR) 的大脑图像。这为人类专家提供了一种方法, 对美国的探针成像性能进行视觉定性评估, 并比较不同探头所做的收购。此外, 该协议依赖于使用一个完整的和开放的研究和开发系统的美国图像采集,即超声高级开放平台 (ULA OP) 扫描仪。
手稿详细描述了协议中涉及的仪器和程序, 特别是对美国和 MR 图像的校准、图像采集和注册。得到的结果证明了整个协议的有效性, 这是完全开放的 (在所涉及的仪器的限制范围内), 可重复, 并涵盖了整个集的收购和处理活动的美国图像。

通过所述协议获得的主要结果是对基于 CMUT 技术的美国探针原型的2D 和3D 成像能力的一种有效且可重复的评估程序的实验验证, 在前瞻性应用于大脑成像.在实现所有描述的协议步骤之后, 专家可以应用可视化软件功能 (例如自由方向切片、子集提取、卷内插、等) 来比较注册的可视化内容美国图像与目标 MR 图像。特别是, 所获得的图像质量, 并与 MRI 的金标准直...

Watch this Scientific Journal Video about An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging at JoVE.com

An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging

基于电容式微机械超声换能器 (CMUT) 技术的新型超声探头的研制需要对成像能力进行早期逼真的评估。我们描述了一个可重复的实验协议, 美国图像采集和与磁共振图像的比较, 使用一个前体内牛脑作为成像靶。

基于 CMUT 技术的新型超声探头在脑成像中的应用研究

The possibility to perform an early and repeatable assessment of imaging performance is fundamental in the design and development process of new ...

Research

JoVE Journal

Bioengineering

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

创建的胶粘剂和可溶性梯度荧光显微镜成像细胞迁移

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High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

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TruTip is a simple nucleic acid extraction  technology whereby a porous, monolithic binding matrix is inserted into a  pipette tip. The geometry of the ...

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Optical sensors of ultrasound are a promising alternative to piezoelectric techniques, as has been recently demonstrated in the field of optoacoustic ...

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

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The objective of this video protocol is to discuss how to perform and analyze a three-dimensional fluorescent orbital particle tracking experiment using a ...

In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology

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A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging

A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging

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In vivo small-animal imaging has an important role to play in preclinical studies. Photoacoustic tomography (PAT) is an emerging hybrid imaging modality ...

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

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Organs-on-chips, in vitro models involving the culture of (human) tissues inside microfluidic devices, are rapidly emerging and promise to provide useful ...

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Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver ...

A New Ex Vivo Model for the Evaluation of Endoscopic Submucosal Injection Material Performance

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内镜下黏膜下注射材料性能评价的一种新的体内模型

Increasing the performance of submucosal injection materials (SIMs) is important for endoscopic therapy of early gastrointestinal cancer. It is essential ...