Name: an experimental protocol for assessing the performance of new ultrasound probes based on cmut technology in application to brain imaging
Uploaded: 2017-09-24T13:00:00
Description: Watch this Scientific Journal Video about An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging at JoVE.com

Dette arbeidet har vært delvis støttet av nasjonale myndigheter og EU gjennom ENIAC JU prosjektet DeNeCoR under grant avtalenummer 324257. Forfatterne vil takke Prof Giovanni Magenes, Prof Piero Tortoli og Dr. Giosuè Caliano for sine dyrebare støtte, veiledning og innsiktsfulle kommentarer som dette arbeidet. Vi er også takknemlige Prof Egidio D'Angelo og hans gruppe (Blindkopi Lab.), sammen med Fondazione Istituto Neurologico C. Mondino, for å gi bevegelse sporing og MR instrumentering, og Giancarlo Germani for MR oppkjøp. Til slutt, vi vil gjerne takke Dr. Nicoletta Caramia, Dr. Alessandro Dallai og fru Barbara Mauti for deres verdifulle kundestøtte og Mr. Walter Volpi for å gi bovin hjernen.

alle biologiske prøver vist i denne videoen har anskaffet gjennom leverandørkjeden standard mat. Disse prøvene er blitt behandlet i henhold til etiske og sikkerhet institusjoner involvert.
 Merk: diagrammet i Figur 4 oppsummerer de 8 viktigste stadiene av denne protokollen. Trinn 1 til 4 involverer innledende aktiviteter, utføres bare én gang før begynnelsen av amerikanske bildeopptak og behandling stadier. Disse første stadiene er som følger: 1. forelø...

<ol>
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Flere har blitt presentert i litteraturen som beskriver teknikker som er lik eller relatert til protokollen presentert. Disse teknikkene er også basert på bruk av realistiske mål, inkludert fast dyr eller cadaver hjernen, men de er hovedsakelig unnfanget for testing av digitale registrering metoder av ulike slag.
Protokollen beskrevet her, men har spesifikke formål å teste amerikanske sonder i forskjellige konfigurasjoner i de tidlige stadiene av utviklingen, og på grunn av dette, det op...

<table border="0" cellpadding="0" cellspacing="0" width="925">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">ULA-OP</td>
			<td style="width:167px;">University of Florence</td>
			<td style="width:104px;">N/A</td>
			<td style="width:415px;">Ultrasound imaging research system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">3D imaging piezeoelectric probe</td>
			<td style="width:167px;">Esaote s.p.a.</td>
			<td style="width:104px;">9600195000</td>
			<td>Mechanically-swept 3D ultrasound probe, model BL-433</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Linear-array piezoelectric probe</td>
			<td style="width:167px;">Esaote s.p.a.</td>
			<td style="width:104px;">122001100</td>
			<td>Ultrasound linear array probe, model LA-533</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">CMUT probe</td>
			<td style="width:167px;">University Roma Tre</td>
			<td style="width:104px;">N/A</td>
			<td>Ultrasound linear array probe based on CMUT technology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">MAGNETOM Skyra 3T MR scanner</td>
			<td style="width:167px;">Siemens Healthcare</td>
			<td style="width:104px;">N/A</td>
			<td>MR scanner</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Head coil</td>
			<td style="width:167px;">Siemens Healthcare</td>
			<td style="width:104px;">N/A</td>
			<td>32-channel head coil for MR imaging</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">NDI Polaris Vicra</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">8700335001</td>
			<td>Optical motion tracking system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Pointer tool</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">8700340</td>
			<td>Passive pointer tool with 4 reflecting markers</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Clamp-equipped tool</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">8700399</td>
			<td>Rigid body with 4 reflecting markers and a clamp to be connected to the US probe handle</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Bovine brain</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Brain of an adult bovine, from food suppliers</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Formalin solution</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>10% buffered formalin solution for bovine brain fixation - CAUTION, formalin is a toxic chemical substance and must be handled with care; specific regulations may also apply (see for instance US OSHA Standard 1910.1048 App A)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Plastic container for anatomical parts</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Cilindrical plastic container with lid</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Glass spheres</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>3mm diameter spheres of Flint glass</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Agar</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>30 g, for phantom preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Glycerine</td>
			<td style="width:167px;">AEFFE Farmaceutici</td>
			<td style="width:104px;">A908005248</td>
			<td>100 g, for phantom preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Distilled water</td>
			<td style="width:167px;">Solbat Gaysol</td>
			<td style="width:104px;">8027391000015</td>
			<td>870 g, for phantom preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Beaker</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Beaker used for the diluition of glycerine and agar in distilled water</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Lysoform</td>
			<td style="width:167px;">Lever</td>
			<td style="width:104px;">8000680500014</td>
			<td>A benzalkonium chloride and water solution was used for the agar phantom preservation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Polystyrene mannequin head</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Polyestirene model which was cutted and used to design the configuration of spheres&#39;patterns</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Green tissue marking dye for histology</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Colour used to mark the glass spheres&#39; positions on the bovine brain surface</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Yellow enamel</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Enamel used to colour the glass spheres implanted in the agar phantom</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Water tank</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>50x50x30 cm plastic tank filled degassed water up to a 15cm height&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Mechanical arm</td>
			<td style="width:167px;">Esaote s.p.a.</td>
			<td style="width:104px;">N/A</td>
			<td>Mechanical arm clamped to the water tank border and used to held the probe in fixed positions</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Plate of synthetic resin</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Plate used as a support for the bovine brain positioning in the water tank</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Sewing threads</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Sewing thread segments used to immobilize the brain on the resin plate</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Adhesive tape</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Adhesive tape used to fix the sewing thread extremities onto the resin plate</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Plastic food container</td>
			<td style="width:167px;">N/A</td>
			<td style="width:104px;">N/A</td>
			<td>Sealed food container used for the agar phantom</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Notebook</td>
			<td style="width:167px;">Lenovo</td>
			<td style="width:104px;">Z50-70</td>
			<td>Lenovo&#160; Z50-70, Intel(R) Core i7-4510U @ 2.0 GHz, 8 GB RAM</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Workstation</td>
			<td style="width:167px;">Dell Inc.</td>
			<td style="width:104px;">T5810</td>
			<td>Intel(R) Xeon(R) CPU E3-1240v3 @ 3.40 GHz, 16 GB RAM</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Matlab</td>
			<td style="width:167px;">The MathWorks</td>
			<td style="width:104px;">R2013a</td>
			<td>Software tool, used for space transformation computation and 3D reconstruction from image planes</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">Paraview</td>
			<td style="width:167px;">Kitware Inc.</td>
			<td style="width:104px;">v. 4.4.1</td>
			<td>Open-source software for 3D image processing and visualization</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">NDI Toolbox - ToolTracker Utility</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">v. 4.007.007</td>
			<td>Software for marker position visualization and tracking in the NDI Polaris Vicra measurement volume</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">C++ data-logging software</td>
			<td style="width:167px;">NDI Medical</td>
			<td style="width:104px;">v. 4.007.007</td>
			<td>Software for marker position recording on a text log file</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:240px;">ULA-OP software&#160;</td>
			<td style="width:167px;">University of Florence</td>
			<td style="width:104px;">N/A</td>
			<td>Software for real-time display and control of the ULA-OP system</td>
		</tr>
	</tbody>
</table>

an experimental protocol for assessing the performance of new ultrasound probes based on cmut technology in application to brain imaging

Muligheten til å utføre en tidlig og repeterbare vurdering av imaging ytelse er grunnleggende i design og utviklingsprosessen av nye ultralyd (US) sonder. Spesielt kan en mer realistisk analyse med programspesifikke tenkelig mål være svært verdifullt å vurdere den forventede ytelsen for amerikanske sonder i deres potensielle klinisk anvendelse.
Eksperimentelle protokollen presentert i dette arbeidet var bevisst utformet for å gi en programspesifikk prosedyre for nyutviklet oss undersøke prototyper basert på kapasitive Micromachined Ultrasonic svinger (CMUT) teknologien i forhold til hjernen imaging.
Protokollen kombinerer bruk av bovin hjernen fast i formalin tenkelig målet, som sikrer både realisme og repeterbarhet beskrevet prosedyrer og neuronavigation teknikker lånt fra nevrokirurgi. Amerikanske sonden er faktisk koblet til en bevegelse sporingssystem som kjøper posisjonsdata og aktiverer superposisjon av amerikanske bilder referanse magnetisk resonans (MR) bilder av hjernen. Dette gir et middel for menneskelig eksperter utfører en visuell kvalitativ vurdering av amerikanske sonden imaging ytelse og sammenligne oppkjøp gjort med forskjellige sonder. Videre avhengig protokollen bruken av et komplett og åpent forskning og utvikling system for US bildeopptak, dvs. ultralyd avanserte åpne plattformen (ULA-OP) skanneren.
Manuskriptet beskriver i detalj de instrumenter og prosedyrer som er involvert i protokollen, spesielt for kalibrering, bildeopptak og registrering av USA og MR bilder. Innhentet resultatene bevise effektiviteten av generelle protokollen presentert, som er helt åpen (innenfor rammene av instrumentering involvert), gjentas, og dekker hele settet og bearbeidingen aktiviteter for amerikanske bilder.

De viktigste resultatet oppnådd via beskrevet protokollen er eksperimentell valideringen av en effektiv og repeterbare prosedyre for 2D og 3D tenkelig evnene av oss undersøke prototyper basert på CMUT teknologi, i programmet potensielle hjernen bildebehandling. Etter å implementere alle trinnene som beskrives protokollen, ekspert kan deretter bruke visualisering programvarefunksjoner (f.eks gratis retning kutting, delsett utvinning, volum interpolering, etc.) for å ...

Watch this Scientific Journal Video about An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging at JoVE.com

An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging

Utviklingen av nye ultralyd (oss)-sonder basert på kapasitive Micromachined Ultrasonic svinger (CMUT) teknologien krever en tidlig realistisk vurdering av imaging evner. Vi beskriver en repeterbare eksperimentelle protokoll for amerikanske bildeopptak og sammenligning med magnetisk resonans bilder, bruke en ex vivo bovin hjernen som en tenkelig mål.

En eksperimentell protokoll for taksere arbeidsinnsatsen av nye ultralyd sonder basert på CMUT teknologi i programmet hjernen bildebehandling

The possibility to perform an early and repeatable assessment of imaging performance is fundamental in the design and development process of new ...

Research

JoVE Journal

Bioengineering

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Opprette Lim og Løselig overgangar for Imaging Cell Migration med fluorescens mikroskopi

Cells can sense and migrate towards higher concentrations of adhesive cues such as the glycoproteins of the extracellular matrix and soluble cues such as ...

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Høy gjennomstrømning, Automated Utvinning av DNA og RNA fra kliniske prøver ved hjelp TruTip Technology på Common Væskehåndtering Robots

TruTip is a simple nucleic acid extraction  technology whereby a porous, monolithic binding matrix is inserted into a  pipette tip. The geometry of the ...

Wideband Optical Detector of Ultrasound for Medical Imaging Applications

Wideband optisk detektor av ultralyd for Medical Imaging Applications

Optical sensors of ultrasound are a promising alternative to piezoelectric techniques, as has been recently demonstrated in the field of optoacoustic ...

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking i en modifisert to-foton Microscope: en søknad til sporing av intracellulær Blemmer

The objective of this video protocol is to discuss how to perform and analyze a three-dimensional fluorescent orbital particle tracking experiment using a ...

In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology

In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology

In Situ Kartlegging av de mekaniske egenskapene til Biofilm av Partikkel-sporing Microrheology

Bacterial cells are able to form surface-attached biofilm communities known as biofilms by encasing themselves in extracellular polymeric substances ...

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage

En eksperimentell og Finite Element-protokollen for å undersøke Transport av nøytrale og siktet Oppløste stoffer over Leddbrusk

Osteoarthritis (OA) is a debilitating disease that is associated with degeneration of articular cartilage and subchondral bone. Degeneration of articular ...

A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging

A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging

Et høyytelses kompakt fotoakustisk tomografisystem for<em&gt; In vivo</em&gt; Små dyrs hjernedannelse

In vivo small-animal imaging has an important role to play in preclinical studies. Photoacoustic tomography (PAT) is an emerging hybrid imaging modality ...

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

Fabrikasjon og validering av en Organ-on-chip System med integrerte elektrodene direkte kvantifisere Transendothelial elektrisk motstand

Organs-on-chips, in vitro models involving the culture of (human) tissues inside microfluidic devices, are rapidly emerging and promise to provide useful ...

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

En kirurgiske teknikken som grunnlag for i Vivo delvis leveren Engineering i rotte

Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver ...

A New Ex Vivo Model for the Evaluation of Endoscopic Submucosal Injection Material Performance

A New Ex Vivo Model for the Evaluation of Endoscopic Submucosal Injection Material Performance

En ny Ex Vivo modell for vurdering av endoskopisk Submucosal injeksjon materiale ytelse

Increasing the performance of submucosal injection materials (SIMs) is important for endoscopic therapy of early gastrointestinal cancer. It is essential ...