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DOI :
10.3791/55802-v
•
11:17 min
June 1st, 2017
Chapters
0:05
Title
10:40
Conclusion
8:51
Results: Representative Chromatograms of Lipid, Semi-polar Metabolites and Proteins from Arabidopsis thaliana Leaf Extracts
1:05
Grinding and Tissue Disruption
2:30
Aliquoting Tissue
3:18
Extraction and Fractionation by Phase Separation
4:56
Aliquoting Polar and Hydrophobic Fractions
6:24
Concentrations and Storage of Fraction and Analysis of Lipids Using UPLC-MS
7:48
Analysis of Polar and Semi-polar Metabolites Using UPLC-MS
Ett protokoll för omfattande extraktion av lipider, metaboliter och proteiner från biologiska vävnader med ett prov presenteras.
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