Name: reproducible motor deficit following aortic occlusion in a rat model of spinal cord ischemia
Uploaded: 2017-07-22T13:00:00
Description: Watch this Scientific Journal Video about Reproducible Motor Deficit Following Aortic Occlusion in a Rat Model Of Spinal Cord Ischemia at JoVE.com

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本议定书由首尔国立大学邦唐医院机构动物护理使用委员会批准。根据美国国家卫生研究院护理和使用实验动物的指南进行动物护理和实验。 手术准备<ol><li>在手术前，用无菌盐水冲洗导管以确保通畅。 </li><li>将加热毯放在手术台上，并用无菌悬垂盖住桌子。 </li><li>将雄性Sprague-Dawley大鼠（270-330g）放入具有3.0％-4.0％异氟烷的丙烯酸盒中，在100％氧气中。 </li><li>向大鼠的?...

<ol>
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在目前的研究中，我们展示了一种基于Taira和Marsala方法5的脊髓缺血大鼠模型，其根据主动脉闭塞时间在后肢中引起不同程度的运动障碍。 主动脉闭塞的长度可影响运动障碍的程度。如果主动脉闭塞时间较长，运动缺陷变得更加严重。因此，研究人员可以通过控制该模型中的主动脉闭塞时间来实现一定程度的运动障碍。 我们的模型涉及?...

截瘫是胸腹主动脉瘤手术的致命并发症。这是由于在主动脉夹紧和松开过程中发生的脊髓缺血 - 再灌注损伤。 1个几种策略包括全身体温过低和脑脊液引流已被引入到保护脊髓，2，3，4，但许多患者仍然受到伤病影响。 引入了几种动物脊髓缺血模型来研究其发病机制，制定了对损伤的保护策略。在本研究中，我们概述了基于Taira和Marsala方法的脊髓缺血大鼠模型。 5大鼠的脊髓循环系统与人类脊髓血管和侧支系统非常相似，尽管大小和位置。 6,7因此，大鼠是解剖学上合适的动物，以利用用于实验模型调查发病，并发症和治疗脊髓缺血的。此外，这种脊髓缺血模型通过利用胸主动脉的血管内气囊闭塞以最小的干预产生可靠的主动脉闭塞。 在这项研究中，我们证明，这种脊髓缺血大鼠模型诱导后肢的可重复的运动缺陷，其严重程度随主动脉闭塞时间而变化。

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			<td height="21" style="height:21px;width:269px;">Fogarty Arterial Embolectomy catheter</td>
			<td style="width:181px;">Edward Life Sciences</td>
			<td style="width:147px;">120602F</td>
			<td style="width:413px;">a balloon-tipped catheter inserted into the femoral artery</td>
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		<tr height="42">
			<td height="42" style="height:42px;width:269px;">BD Insyte-N Autoguard Shielded IV catheter&#160;</td>
			<td style="width:181px;">BD&#160;</td>
			<td style="width:147px;">381411</td>
			<td>24-gauge intravenous catheter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">50mL syringe</td>
			<td style="width:181px;">KOREA VACCINE&#160;</td>
			<td>KOVAX-SYRINGE 50mL</td>
			<td>Facial mask</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">1mL syringe</td>
			<td style="width:181px;">KOREA VACCINE</td>
			<td>KOVAX-SYRINGE 1ml</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:269px;">Recal probe</td>
			<td>HARVARD APPARATUS</td>
			<td>50-7221F</td>
			<td>Rectal probe for temperature monitoring</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micro dissecting spring scissor</td>
			<td>Jeung do bio &#38; Plant co.LTD.</td>
			<td>JD-S-10</td>
			<td>Micro-scissor</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">SCISSOR (SHARP-SHARP)</td>
			<td>Jeung do bio &#38; Plant co.LTD.</td>
			<td>S-51-12-S</td>
			<td>Scissors</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Retractor</td>
			<td>Jeung do bio &#38; Plant co.LTD.</td>
			<td>JD-S-74A</td>
			<td>Retractor</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micro forcep&#160;</td>
			<td>Jeung do bio &#38; Plant co.LTD.</td>
			<td>JD-S-29</td>
			<td>Micro-forceps</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:269px;">MOSQUITO FORCEP (Curved)</td>
			<td>Jeung do bio &#38; Plant co.LTD.</td>
			<td>S-44-CPK</td>
			<td>Curved forceps</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">DRESSING FORCEP&#160;</td>
			<td>Jeung do bio &#38; Plant co.LTD.</td>
			<td>S-37-16S</td>
			<td>Blunted forceps</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">4/0 black silk&#160;</td>
			<td>Woori Medical</td>
			<td style="width:147px;">S431</td>
			<td>4.0 black silk suture</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">3-WAY STOCK</td>
			<td style="width:181px;">Seonwon Medcal</td>
			<td>D-98-01</td>
			<td>3-way stopcock</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Patient monitor</td>
			<td>PHILIPS</td>
			<td style="width:147px;">MP20</td>
			<td>The arterial pressure monitoring device.&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Heating blanket</td>
			<td style="width:181px;">Self production</td>
			<td style="width:147px;"></td>
			<td>Heating blanket</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Microtube and external reservoir</td>
			<td style="width:181px;">Self production</td>
			<td style="width:147px;"></td>
			<td>Microtube and external reservoir</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Heparin</td>
			<td style="width:181px;">JW Pharmaceutical</td>
			<td style="width:147px;"></td>
			<td>Heparin</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">0.9% NS 1000ml</td>
			<td style="width:181px;">JW Pharmaceutical</td>
			<td style="width:147px;"></td>
			<td>Normal saline</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Isoflurane</td>
			<td style="width:181px;">Hana Med</td>
			<td style="width:147px;"></td>
			<td>Isoflurane</td>
		</tr>
	</tbody>
</table>

reproducible motor deficit following aortic occlusion in a rat model of spinal cord ischemia

<html>脊髓缺血是胸腹主动脉瘤手术后的致命并发症。研究人员可以使用脊髓缺血实验模型调查预防和治疗这种并发症的策略。这里描述的模型表明与大鼠脊髓缺血模型中胸主动脉闭塞后的闭塞长度有关的不同程度的截瘫。 2-Fr。气球导管通过股动脉进入下行胸主动脉，直到导管尖端置于麻醉的雄性Sprague-Dawley大鼠的左锁骨下动脉中。通过使导管气囊膨胀来诱导脊髓缺血。经过一段闭塞时间（9,10或11分钟）后，气囊放气。术后24 h采用运动缺陷指数进行神经病理学评估，收集脊髓进行组织病理学检查。 主动脉闭塞9分钟的大鼠在后肢显示出轻度且可逆的运动障碍，经受10分钟主动脉闭塞的大鼠出现中度但可逆运动障碍，经受主动脉闭塞11分钟的大鼠显示完全和持续瘫痪，大鼠主动脉闭塞持续时间较长的大鼠脊髓部分运动神经元更保守。 使用这种脊髓缺血模型，研究人员可以在胸主动脉闭塞后达到可重现的后肢运动障碍。

在脊髓缺血期间，主动脉闭塞9分钟（n = 3），10分钟（n = 3）或11分钟（n = 3）。大鼠的运动缺陷指数见表2.主动脉闭塞9分钟的大鼠在后肢显示轻度和可逆的运动障碍。大鼠经历10分钟的主动脉闭塞呈现中度运动缺陷，但不完全麻痹。经历11分钟闭塞时间的大鼠显示完全和持续性麻痹。 用H＆E染色的脊髓切片的代表性照片如图1所示。在经历较短持续时间的?...

Watch this Scientific Journal Video about Reproducible Motor Deficit Following Aortic Occlusion in a Rat Model Of Spinal Cord Ischemia at JoVE.com

Reproducible Motor Deficit Following Aortic Occlusion in a Rat Model Of Spinal Cord Ischemia

本研究显示了在大鼠中进行脊髓缺血的微创和易复制模型的技术。通过控制主动脉闭塞时间可以产生不同程度的后肢运动障碍。

主动脉阻塞后脊髓缺血大鼠模型中的可再生电机缺陷

Spinal cord ischemia is a fatal complication following thoracoabdominal aortic aneurysm surgery. Researchers can investigate the strategies for preventing ...

Research

JoVE Journal

Medicine

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

在大鼠脊髓损伤后的急性和慢性触觉测试

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

科研

医学

A Contusion Model of Severe Spinal Cord Injury in Rats

挫伤模型脊髓严重损伤的保护作用

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia

2血管闭塞/低血压：全脑缺血模型大鼠

Cardiac arrest followed by resuscitation often results in dramatic brain damage caused by ischemia and subsequent reperfusion of the brain. Global brain ...

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment (RMCA) in Individuals with Chronic Spinal Cord Injury

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment RMCA in Individuals with Chronic Spinal Cord Injury

评价呼吸肌慢性脊髓损伤个人使用呼吸电机控制评估（RMCA）

During breathing, activation of respiratory muscles is coordinated by integrated input from the brain, brainstem, and spinal cord. When this coordination ...

Reproducable Paraplegia by Thoracic Aortic Occlusion in a Murine Model of Spinal Cord Ischemia-reperfusion

可重放截瘫胸主动脉阻断脊髓缺血再灌注的小鼠模型

Background
Lower extremity paralysis continues to complicate aortic interventions. The lack of understanding of the underlying pathology has hindered ...

Controlled Cortical Impact Model for Traumatic Brain Injury

控制皮层影响模型创伤性脑损伤

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

无线电遥测系统，以监测大鼠脊髓横断和胚胎神经干细胞移植在心血管功能

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Photothrombosis-induced Focal Ischemia as a Model of Spinal Cord Injury in Mice

Photothrombosis引起的局部脑缺血脊髓损伤的小鼠模型

Spinal cord injury (SCI) is a devastating clinical condition causing permanent changes in sensorimotor and autonomic functions of the spinal cord (SC) ...

A Neonatal Mouse Spinal Cord Compression Injury Model

新生儿小鼠脊髓压迫损伤模型

Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal ...