Name: expansion and adipogenesis induction of adipocyte progenitors from perivascular adipose tissue isolated by magnetic activated cell sorting
Uploaded: 2017-06-30T14:00:00
Description: Watch this Scientific Journal Video about Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting at JoVE.com

Contreras og Watts Laboratories og Dr. William Raphael. Disse eksperimenter blev understøttet af NHLBI F31 HL128035-01 (vævsfordøjelsesprotokol standardisering), NHLBI 5R01HL117847-02 og 2P01HL070687-11A1 (dyr) og NHLBI 5R01HL117847-02 (celleisolering og kultur).

Alle procedurer beskrevet i dette papir følger retningslinjer fastlagt af Institutt for Dyrepleje og Brugskomité (IACUC) fra Michigan State University. Alle buffere og medier skal beskyttes mod lys. 1. Fremstilling af buffere, medier og instrumenter <ol><li> Forbered Krebs Ringer Bicarbonate-Buffered Solution (KRBB): 135 mM natriumchlorid, 5 mM kaliumchlorid, 1 mM magnesiumsulfat, 0,4 mM kaliumphosphatdibasisk, 5,5 mM glucose, 1% antibiotisk / antimykotisk (10.000 enheder / ml penicillin...

<ol>
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A., Thelen, K., Ayala-Lopez, N., Watts, S. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+distribution+and+adipogenic+potential+of+perivascular+adipose+tissue+adipocyte+progenitors+is+dependent+on+sexual+dimorphism+and+vessel+location.">The distribution and adipogenic potential of perivascular adipose tissue adipocyte progenitors is dependent on sexual dimorphism and vessel location.</a> Physiol Rep. 4 (19), (2016).</li><li>Lee, Y. H., Petkova, A. P., Granneman, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+an+adipogenic+niche+for+adipose+tissue+remodeling+and+restoration.">Identification of an adipogenic niche for adipose tissue remodeling and restoration.</a> Cell Metab. 18 (3), 355-367 (2013).</li><li>Lee, Y. H., Petkova, A. P., Mottillo, E. P., Granneman, J. 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Det centrale fokus i det foreliggende eksperiment er isoleringen, ekspansion og adipogen induktion af APC fra PVAT depot. Her præsenterer vi en protokol til isolering af APC baseret på identifikationen af ​​celler, der udtrykker overflademarkørerne CD34 og PDGFRα. Disse overfladeproteiner blev tidligere identificeret på APC med høje proliferationshastigheder og potentialet til at differentiere i hvide eller brune adipocytter i forskellige adipose depots 14 , <sup class="xref"...

Perivaskulær Adipose Tissue (PVAT), på grund af dens nærhed til blodkar, er en vigtig parakrin signalering komponent i vaskulatur funktion 1 . Udvidelse af dette fedtvæv afhænger af fænotypen af ​​adipocytprogenitorcellerne (APC) til stede 2 , 3 . Isolering af celler fra fedtvæv er vanskelig, da primære adipocytter er skrøbelige, flydende og varierende i størrelse. Visse isolationsteknikker kan også ændre cellefænotype og morfologi ved at øge inflammatorisk proteinsyntese og reducere adipogen genekspression 4 , idet man understreger vigtigheden af ​​en protokol, som opretholder cellernes integritet. Kultur af primære celler og specifikke preadipocyt subpopulationer giver en reduktionistisk tilgang til in vivo vækst og opretholder ækvivalent cellulær genetisk makeup 5 , selvom man arbejder tiMig med disse celler er begrænset på grund af forringelse med aldring eller senescence 6 . Preadipocytter fra forskellige fedtdepoter, herunder subkutane og omentale depoter, viser også forskelle i proliferation 7 , hvilket understreger vigtigheden af ​​at samle celler fra specifikke anatomiske steder. Prækursorceller fra ikke-PVAT hvide fedtdepoter er blevet karakteriseret i tidligere undersøgelser 7 , 8 , 9 , men mindre er kendt om PVAT APC-fænotyper. De her beskrevne teknikker muliggør analyse af specifikke og definerede APC-populationer med minimal indvirkning på deres morfologi, levedygtighed og potentiale til proliferation og differentiering. Magnetisk aktiveret cellesortering (MCS) er tilgængelig for downstream-applikationer, såsom kultur, da perlerne opløses uden at ændre cellen. MCS er også økonomisk, og når antistoffet conCentrationer er standardiseret, er behovet for flowcytometrianalyser minimal. In vitro- undersøgelser med PVAT-precursorer kan også give et glimt af det potentiale, som disse primære celler kan have.

<table border="0" cellpadding="0" cellspacing="0" width="757">
	<colgroup>
		<col />
		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="20">
			<td height="20" style="height:20px;width:325px;">Tissue Dissection</td>
			<td style="width:168px;"></td>
			<td style="width:108px;"></td>
			<td style="width:156px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Dissecting Dishes</td>
			<td>Handmade with Silicone</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Culture Petri Dish</td>
			<td>Pyrex</td>
			<td></td>
			<td>7740 Glass</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Silicone Elastomer</td>
			<td>Dow Corning</td>
			<td>Sylgard 170</td>
			<td>Kit</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Braided Silk Suture</td>
			<td>Harvard Apparatus</td>
			<td>51-7615</td>
			<td>SP104</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Stereomicroscope MZ6</td>
			<td>Leica</td>
			<td></td>
			<td>10447254</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Stereomaster Microscope Fiber-Optic Light Source</td>
			<td>Fisher Scientific</td>
			<td>12562-36</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Vannas Scissors</td>
			<td>George Tiemann &#38; Co</td>
			<td>160-150</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Splinter Forceps</td>
			<td>George Tiemann &#38; Co</td>
			<td>160-55</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tissue Scissors</td>
			<td>George Tiemann &#38; Co</td>
			<td>105-400</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">KRBB Solution</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium Chloride</td>
			<td>Sigma-Aldrich</td>
			<td>7647-14-5</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Potassium Chloride</td>
			<td>Sigma-Aldrich</td>
			<td>7447-40-7</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Magnesium Sulfate</td>
			<td>Sigma-Aldrich</td>
			<td>7487-88-9</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Potassium Phosphate Dibasic</td>
			<td>Sigma-Aldrich</td>
			<td>7758-114</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Glucose</td>
			<td>Sigma-Aldrich</td>
			<td>50-99-7</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Antibiotic/Antimicotic</td>
			<td>Corning</td>
			<td>30-004-CI</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">HEPES</td>
			<td>Corning</td>
			<td>25-060-CI</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tissue Digestion</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Collagenase Type 1</td>
			<td>Worthington Biochemical</td>
			<td>LS004196</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Bovine Serum Albumin</td>
			<td>Fisher Scientific</td>
			<td>9048-46-8</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Red Blood Cell Lysis Buffer</td>
			<td>BioLegend</td>
			<td>420301</td>
			<td>1X Working Solution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Water Bath</td>
			<td>Thermo-Fisher Scientific</td>
			<td>2876</td>
			<td>Reciprocal Shaking Bath</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Biosafety Cabinet</td>
			<td>Thermo-Fisher Scientific</td>
			<td>1385</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Rotisserie Incubator</td>
			<td>Daigger</td>
			<td>EF4894C</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">100 &#181;m Cell Strainer</td>
			<td>Thermo-Fisher Scientific</td>
			<td>22-363-549</td>
			<td>Yellow</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">40 &#181;m Cell Strainer</td>
			<td>Thermo-Fisher Scientific</td>
			<td>22-363-547</td>
			<td>Blue</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hemocytometer</td>
			<td>Cole-Parmer</td>
			<td>UX-79001-00</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Trypan Blue</td>
			<td>Sigma-Aldrich</td>
			<td>93595</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cell Isolation</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">OctoMACS Kit</td>
			<td>Miltenyi Biotech</td>
			<td>130-042-108</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">(DMEM):F12 Medium</td>
			<td>Corning</td>
			<td>90-090</td>
			<td>Medium Base</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fetal Bovine Serum</td>
			<td>Corning</td>
			<td>35016CV</td>
			<td>USA Origins</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Normal Donkey Serum</td>
			<td>AbCam</td>
			<td>AB7475</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Anti-CD34</td>
			<td>Santa Cruz</td>
			<td>SC-7324</td>
			<td>FITC conjugated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Anti-PDGFR&#945;</td>
			<td>Thermo-Fisher Scientific</td>
			<td>PA5-17623</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Donkey Anti-Rabbit IgG</td>
			<td>Jackson ImmunoResearch</td>
			<td>712-007-003</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">PBS 10X</td>
			<td>Corning</td>
			<td>46-013-CM</td>
			<td>1X Working Solution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">EDTA</td>
			<td>Fisher Scientific</td>
			<td>15575020</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cell Culture</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">CO2 Cell Incubator</td>
			<td>Thermo-Fisher Scientific</td>
			<td>51030285</td>
			<td>Heracell 160i&#160;</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">6-Well Plates</td>
			<td>Corning</td>
			<td>3516</td>
			<td>TC-Treated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">48- Well Plates</td>
			<td>Corning</td>
			<td>3548</td>
			<td>TC-Treated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">96-Well Plates, Black Wall</td>
			<td>Corning</td>
			<td>353376</td>
			<td>TC-Treated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium Bicarbonate</td>
			<td>Sigma-Aldrich</td>
			<td>144-55-8</td>
			<td>TC-Treated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fetal Calf Serum</td>
			<td>Corning</td>
			<td>35011CV</td>
			<td>USA Origins</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Ascorbic Acid</td>
			<td>Sigma-Aldrich</td>
			<td>50-81-7</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Biotin</td>
			<td>Sigma-Aldrich</td>
			<td>58-85-5</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Pantothenate</td>
			<td>Sigma-Aldrich</td>
			<td>137-08-6</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">L-Glutamine</td>
			<td>Corning</td>
			<td>61-030</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Bone Morphogenic Protein 4</td>
			<td>Prospec Bio</td>
			<td>CYT-081</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Epidermal Growth Factor</td>
			<td>PeproTech</td>
			<td>400-25</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Leukemia Inhibitory Factor</td>
			<td>PeproTech</td>
			<td>250-02</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Platelet-derived Growth Factor BB</td>
			<td>Prospec Bio</td>
			<td>CYT-740</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Basic Fibroblast Growth Factor</td>
			<td>PeproTech</td>
			<td>450-33</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Insulin</td>
			<td>Corning</td>
			<td>25-800-CR</td>
			<td>ITS Solution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">IBMX</td>
			<td>Sigma-Aldrich</td>
			<td>28822-58-4</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Dexamethasone</td>
			<td>Sigma-Aldrich</td>
			<td>50-02-2</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">T3 (Triiodothyronine)</td>
			<td>Sigma-Aldrich</td>
			<td>6893-023</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cell Analysis</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">CyQUANT Proliferation Assay</td>
			<td>Thermo-Fisher Scientific</td>
			<td>C7026</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">AdipoRed Fluorescence Assay Reagent</td>
			<td>Lonza</td>
			<td>PT-7009</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Oil Red O Lipid Dye Reagent</td>
			<td>Sigma-Aldrich</td>
			<td>O1391</td>
			<td>In Solution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">M1000 Microplate Reader</td>
			<td>Tecan</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Eclipse Inverted Microscope</td>
			<td>Nikon</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Digital Sight DS-Qil Camera</td>
			<td>Nikon</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

expansion and adipogenesis induction of adipocyte progenitors from perivascular adipose tissue isolated by magnetic activated cell sorting

Udvidelse af perivaskulær adiposevæv (PVAT), en vigtig regulator af vaskulær funktion gennem parakrin signalering, er direkte relateret til udviklingen af ​​hypertension under fedme. Omfanget af hypertrofi og hyperplasi afhænger af depotplacering, køn og typen af ​​adipocytprogenitorcelle (APC) -fænotyper til stede. Teknikker anvendt til APC og præadipocytter isolation i de sidste 10 år har drastisk forbedret nøjagtigheden, hvormed enkelte celler kan identificeres baseret på specifikke celleoverflademarkører. Imidlertid kan isolering af APC og adipocytter være en udfordring på grund af cellens skrøbelighed, især hvis den intakte celle beholdes for cellekulturapplikationer. Magnetisk aktiveret cellesortering ( MCS) tilvejebringer en fremgangsmåde til isolering af større antal levedygtige APC pr. Vægt enhed af fedtvæv. APC høstet af MCS kan anvendes til in vitro protokoller for at udvide præaDipocytter og differentiere dem i adipocytter ved anvendelse af vækstfaktor-cocktails, der muliggør analyse af det produktive og adipogene potentiale, der tilbageholdes af cellerne. Dette forsøg fokuserede på de aorta og mesenteriske PVAT depots, som spiller centrale roller i udviklingen af ​​hjerte-kar-sygdomme under ekspansion. Disse protokoller beskriver metoder til at isolere, udvide og differentiere en defineret population af APC. Denne MCS-protokol gør det muligt at anvende isolering i ethvert eksperiment, hvor celle sortering er nødvendig med minimal udstyr eller træning. Disse teknikker kan hjælpe yderligere forsøg til at bestemme funktionaliteten af ​​specifikke cellepopulationer baseret på tilstedeværelsen af ​​celleoverflademarkører.

Proliferativ kapacitet af preadipocytter og adipogen potentiale af adipocytprecursorer er karakteristika, som opretholdes in vitro 11 . In vitro proliferation af isoleret SVF og APC fra aPVAT, mPVAT og GON fra hanrotter blev evalueret ved 8, 24, 48 og 96 timer efter plating ved anvendelse af et kvantitativt DNA-assay. Der blev ikke observeret nogen stedforskelle i SVF-ekspansionshastighed på ethvert tidspunkt, undtagen APC fra aPVAT, som havde m...

Watch this Scientific Journal Video about Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting at JoVE.com

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Her rapporteres en metode til isolering af Adipocyte Progenitor Cell (APC) populationer fra Perivascular Adipose Tissue (PVAT) ved hjælp af magnetisk aktiveret cellesortering (MCS). Denne metode muliggør en øget isolering af APC pr. Gram fedtvæv i sammenligning med fluorescensaktiveret cellesortering (FACS).

Ekspansion og adipogeneseinduktion af adipocytprogenitorer fra perivaskulær adiposevæv isoleret ved magnetisk aktiveret cellesortering

Expansion of Perivascular Adipose Tissue (PVAT), a major regulator of vascular function through paracrine signaling, is directly related to the ...

Research

JoVE Journal

Developmental Biology

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Isolering og differentiering af adipøst afledte stamceller fra Porcine subkutane fedtvæv

Obesity is an unconstrained worldwide epidemic. Unraveling molecular controls in adipose tissue development holds promise to treat obesity or diabetes. ...

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Isolation og udvidelse af mesenchymstamceller / stromacellers afledt af humane Placenta Tissue

Mesenchymal stem/stromal cells (MSC) are promising candidates for use in cell-based therapies. In most cases, therapeutic response appears to be cell-dose ...

Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue

Isolering af Perivaskulær multipotente precursorcelle populationer fra humant hjertevæv

Multipotent mesenchymal stem/stromal cells (MSC) were conventionally isolated, through their plastic adherence, from primary tissue digests whilst their ...

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Induktion af akut Skeletal Muscle Regeneration af cardiotoxin Injection

Skeletal muscle regeneration is a physiological process that occurs in adult skeletal muscles in response to injury or disease. Acute injury-induced ...

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Chondrogene differentiering Induktion af adipøst afledte stamceller ved centrifugal Gravity

Impaired cartilage cannot heal naturally. Currently, the most advanced therapy for defects in cartilage is the transplantation of chondrocytes ...

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Identifikation af erythromyeloidprogenitorer og deres afkom i musembryoen ved flowcytometri

Macrophages are professional phagocytes from the innate arm of the immune system. In steady-state, sessile macrophages are found in adult tissues where ...

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

Hele-celle patch-clamp optagelser af isolerede primære epitelceller fra epididymis

The epididymis is an essential organ for sperm maturation and reproductive health. The epididymal epithelium consists of intricately connected cell types ...

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Generation af stillads-fri, tre-dimensionelle Insulin udtrykker Pancreatoids fra mus i bugspytkirtlen stamfaderen In Vitro

The pancreas is a complex organ composed of many different cell types that work together to regulate blood glucose homeostasis and digestion. These cell ...

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Effektiv Generation af bugspytkirtlen/Duodenum HOXD Protein 1+ Posterior Foregut/pancreas ophav fra hPSCs i vedhæftning kulturer

Human pluripotent stem cell (hPSC)-derived pancreatic cells are a promising cell source for regenerative medicine and a platform to study human ...

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Isolation, Kultur og adipogen induktion af neuralcrest original fedt-afledte stamceller fra periaortisk fedtvæv

An excessive amount of adipose tissue surrounding the blood vessels (perivascular adipose tissue, also known as PVAT) is associated with a high risk of ...