Name: expansion and adipogenesis induction of adipocyte progenitors from perivascular adipose tissue isolated by magnetic activated cell sorting
Uploaded: 2017-06-30T14:00:00
Description: Watch this Scientific Journal Video about Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting at JoVE.com

Contreras and Watts Laboratuvarları ve Dr. William Raphael. Bu deneyler NHLBI F31 HL128035-01 (doku sindirim protokol standardizasyonu), NHLBI 5R01HL117847-02 ve 2P01HL070687-11A1 (hayvanlar) ve NHLBI 5R01HL117847-02 (hücre izolasyonu ve kültür) tarafından desteklenmiştir.

Bu yazıda açıklanan tüm prosedürler, Michigan Devlet Üniversitesi&#39;nin Kurumsal Hayvan Bakımı ve Kullanım Komitesi (IACUC) tarafından oluşturulan yönergeleri izlemektedir. Tüm tamponlar ve medyalar ışığa karşı korunmalıdır. 1. Tamponların, Ortamların ve Aletlerin Hazırlanması <ol><li> 135 mM sodyum klorür, 5 mM potasyum klorür, 1 mM magnezyum sülfat, 0.4 mM potasyum fosfat dibazik, 5.5 mM glukoz,% 1 antibiyotik / antimikotik (10,000 ünite / mL penisilin, 10,...

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Bu deneyin odak noktası PVAT depolarından APC&#39;nin izolasyonu, genleşmesi ve adipogenik indüksiyonudur. Burada yüzey işaretleri CD34 ve PDGFRα ifade hücrelerin tanımlanması dayalı APC izolasyonu için bir protokol sunuyoruz. Bu yüzey proteinleri daha önce APC üzerinde yüksek proliferasyon oranları ve çeşitli yağ depoları 14,15&#39;te beyaz veya kahverengi adipositleri ayırma potansiyeli ile tanımlandı. Bu belirteçlere dayalı hücreleri seçerek, seçilen PVAT&#39;de gözlemlenen adiposit feno...

Perivasküler Adipoz Doku (PVAT), kan damarlarına yakın olması nedeniyle damar sistemi fonksiyonu 1&#39;de önemli parakrin sinyal bileşenidir. Bu yağ dokusunun genleşmesi, 2 , 3 mevcut Adiposit Progenitör Hücrelerinin (APC) fenotipine bağlıdır. Primer adipositler kırılgan, yüzen ve aralıklı olduğundan, yağ dokularından hücrelerin izolasyonu zordur. Bazı izolasyon teknikleri aynı zamanda hücrelerin bütünlüğünü koruyan bir protokolün önemini vurgulayan, inflamatuvar protein sentezini arttırarak ve adipogenik gen ekspresyonunu azaltarak hücre fenotipini ve morfolojisini değiştirebilir. Birincil hücrelerin kültürü ve spesifik preadiposit alt popülasyonları, in vivo büyümeye indirgemeci bir yaklaşım sağlar ve eşdeğer hücresel genetik yapıyı korur 5 , ancak tiYaşlanma, ya da yaşlanma 6 ile bozulma nedeniyle bu hücrelerle beni sınırlıdır. Deri altı ve omental depolar da dahil olmak üzere çeşitli yağ depolardan preadipositler, spesifik anatomik bölgelerden hücrelerin toplanmasının önemini vurgulayan proliferasyon 7&#39;deki farklılıkları da gösterir. PVAT dışı beyaz yağ depolarından öncül hücreler önceki çalışmalar 7 , 8 , 9&#39;da karakterize edilmiştir, ancak PVAT APC fenotipleri hakkında daha az bilgi bilinmektedir. Burada açıklanan teknikler, morfolojileri, yaşayabilirlikleri ve çoğalması ve farklılaşması potansiyeli üzerinde asgari düzeyde etkisi olan spesifik ve tanımlanmış APC popülasyonlarının analiz edilmesine izin verir. Manyetik olarak aktive olan Hücre Ayırma (MCS), hücrenin değiştirilmeden boncukların çözünmesi nedeniyle kültür gibi müteakip uygulamalara uygundur. MCS de ekonomiktir ve bir kez antikor koniSantitler standart hale getirildiğinden, akış sitometrisi deneylerine olan ihtiyaç azdır. PVAT öncülleri ile yapılan in vitro çalışmalar, bu primer hücrelerin sahip olabileceği potansiyelin bir görüntüsünü de verebilir.

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			<td height="20" style="height:20px;width:325px;">Tissue Dissection</td>
			<td style="width:168px;"></td>
			<td style="width:108px;"></td>
			<td style="width:156px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Dissecting Dishes</td>
			<td>Handmade with Silicone</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Culture Petri Dish</td>
			<td>Pyrex</td>
			<td></td>
			<td>7740 Glass</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Silicone Elastomer</td>
			<td>Dow Corning</td>
			<td>Sylgard 170</td>
			<td>Kit</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Braided Silk Suture</td>
			<td>Harvard Apparatus</td>
			<td>51-7615</td>
			<td>SP104</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Stereomicroscope MZ6</td>
			<td>Leica</td>
			<td></td>
			<td>10447254</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Stereomaster Microscope Fiber-Optic Light Source</td>
			<td>Fisher Scientific</td>
			<td>12562-36</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Vannas Scissors</td>
			<td>George Tiemann &#38; Co</td>
			<td>160-150</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Splinter Forceps</td>
			<td>George Tiemann &#38; Co</td>
			<td>160-55</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tissue Scissors</td>
			<td>George Tiemann &#38; Co</td>
			<td>105-400</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">KRBB Solution</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium Chloride</td>
			<td>Sigma-Aldrich</td>
			<td>7647-14-5</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Potassium Chloride</td>
			<td>Sigma-Aldrich</td>
			<td>7447-40-7</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Magnesium Sulfate</td>
			<td>Sigma-Aldrich</td>
			<td>7487-88-9</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Potassium Phosphate Dibasic</td>
			<td>Sigma-Aldrich</td>
			<td>7758-114</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Glucose</td>
			<td>Sigma-Aldrich</td>
			<td>50-99-7</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Antibiotic/Antimicotic</td>
			<td>Corning</td>
			<td>30-004-CI</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">HEPES</td>
			<td>Corning</td>
			<td>25-060-CI</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tissue Digestion</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Collagenase Type 1</td>
			<td>Worthington Biochemical</td>
			<td>LS004196</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Bovine Serum Albumin</td>
			<td>Fisher Scientific</td>
			<td>9048-46-8</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Red Blood Cell Lysis Buffer</td>
			<td>BioLegend</td>
			<td>420301</td>
			<td>1X Working Solution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Water Bath</td>
			<td>Thermo-Fisher Scientific</td>
			<td>2876</td>
			<td>Reciprocal Shaking Bath</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Biosafety Cabinet</td>
			<td>Thermo-Fisher Scientific</td>
			<td>1385</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Rotisserie Incubator</td>
			<td>Daigger</td>
			<td>EF4894C</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">100 &#181;m Cell Strainer</td>
			<td>Thermo-Fisher Scientific</td>
			<td>22-363-549</td>
			<td>Yellow</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">40 &#181;m Cell Strainer</td>
			<td>Thermo-Fisher Scientific</td>
			<td>22-363-547</td>
			<td>Blue</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hemocytometer</td>
			<td>Cole-Parmer</td>
			<td>UX-79001-00</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Trypan Blue</td>
			<td>Sigma-Aldrich</td>
			<td>93595</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cell Isolation</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">OctoMACS Kit</td>
			<td>Miltenyi Biotech</td>
			<td>130-042-108</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">(DMEM):F12 Medium</td>
			<td>Corning</td>
			<td>90-090</td>
			<td>Medium Base</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fetal Bovine Serum</td>
			<td>Corning</td>
			<td>35016CV</td>
			<td>USA Origins</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Normal Donkey Serum</td>
			<td>AbCam</td>
			<td>AB7475</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Anti-CD34</td>
			<td>Santa Cruz</td>
			<td>SC-7324</td>
			<td>FITC conjugated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Anti-PDGFR&#945;</td>
			<td>Thermo-Fisher Scientific</td>
			<td>PA5-17623</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Donkey Anti-Rabbit IgG</td>
			<td>Jackson ImmunoResearch</td>
			<td>712-007-003</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">PBS 10X</td>
			<td>Corning</td>
			<td>46-013-CM</td>
			<td>1X Working Solution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">EDTA</td>
			<td>Fisher Scientific</td>
			<td>15575020</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cell Culture</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">CO2 Cell Incubator</td>
			<td>Thermo-Fisher Scientific</td>
			<td>51030285</td>
			<td>Heracell 160i&#160;</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">6-Well Plates</td>
			<td>Corning</td>
			<td>3516</td>
			<td>TC-Treated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">48- Well Plates</td>
			<td>Corning</td>
			<td>3548</td>
			<td>TC-Treated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">96-Well Plates, Black Wall</td>
			<td>Corning</td>
			<td>353376</td>
			<td>TC-Treated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium Bicarbonate</td>
			<td>Sigma-Aldrich</td>
			<td>144-55-8</td>
			<td>TC-Treated</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fetal Calf Serum</td>
			<td>Corning</td>
			<td>35011CV</td>
			<td>USA Origins</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Ascorbic Acid</td>
			<td>Sigma-Aldrich</td>
			<td>50-81-7</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Biotin</td>
			<td>Sigma-Aldrich</td>
			<td>58-85-5</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Pantothenate</td>
			<td>Sigma-Aldrich</td>
			<td>137-08-6</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">L-Glutamine</td>
			<td>Corning</td>
			<td>61-030</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Bone Morphogenic Protein 4</td>
			<td>Prospec Bio</td>
			<td>CYT-081</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Epidermal Growth Factor</td>
			<td>PeproTech</td>
			<td>400-25</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Leukemia Inhibitory Factor</td>
			<td>PeproTech</td>
			<td>250-02</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Platelet-derived Growth Factor BB</td>
			<td>Prospec Bio</td>
			<td>CYT-740</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Basic Fibroblast Growth Factor</td>
			<td>PeproTech</td>
			<td>450-33</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Insulin</td>
			<td>Corning</td>
			<td>25-800-CR</td>
			<td>ITS Solution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">IBMX</td>
			<td>Sigma-Aldrich</td>
			<td>28822-58-4</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Dexamethasone</td>
			<td>Sigma-Aldrich</td>
			<td>50-02-2</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">T3 (Triiodothyronine)</td>
			<td>Sigma-Aldrich</td>
			<td>6893-023</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cell Analysis</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">CyQUANT Proliferation Assay</td>
			<td>Thermo-Fisher Scientific</td>
			<td>C7026</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">AdipoRed Fluorescence Assay Reagent</td>
			<td>Lonza</td>
			<td>PT-7009</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Oil Red O Lipid Dye Reagent</td>
			<td>Sigma-Aldrich</td>
			<td>O1391</td>
			<td>In Solution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">M1000 Microplate Reader</td>
			<td>Tecan</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Eclipse Inverted Microscope</td>
			<td>Nikon</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Digital Sight DS-Qil Camera</td>
			<td>Nikon</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

expansion and adipogenesis induction of adipocyte progenitors from perivascular adipose tissue isolated by magnetic activated cell sorting

Parakrin sinyal vasıtasıyla vasküler fonksiyonların büyük düzenleyicisi olan perivasküler yağ dokusunun (PVAT) genişlemesi, obezite sırasında hipertansiyonun gelişimi ile doğrudan ilişkilidir. Hipertrofi ve hiperplazinin derecesi, depo yer, cinsiyet ve mevcut Adiposit Progenitör Hücre (APC) fenotiplerinin türüne bağlıdır. Son 10 yılda APC ve preadipositler izolasyonu için kullanılan teknikler, spesifik hücre yüzeyi belirteçlerine dayalı olarak tek tek hücrelerin tanımlanabileceği doğruluğunu büyük ölçüde geliştirmiştir. Bununla birlikte, APC ve adipositlerin izolasyonu, özellikle hücrelerin hücre kültürü uygulamaları için muhafaza edilmesi gerekiyorsa, hücrenin kırılganlığından dolayı bir meydan okuma olabilir. Manyetik olarak aktive olan Hücre Ayırma ( MCS), yağ dokusunun ağırlık birimi başına daha fazla canlı APC&#39;yi izole etmek için bir yöntem sağlar. MCS tarafından hasat edilen APC, preapeni genişletmek için in vitro protokoller için kullanılabilirDipositleri ve büyüme faktörü kokteyllerini kullanarak hücreler tarafından tutulan üretken ve adipojenik potansiyelin analizine izin vererek adipositlere ayırdık. Bu deney genişleme sırasında kardiyovasküler hastalığın gelişiminde kilit rol oynayan aortik ve mezenterik PVAT depoları üzerine odaklanmıştır. Bu protokoller, APC&#39;nin tanımlanmış bir popülasyonunu izole etmek, genişletmek ve ayırmak için kullanılan yöntemleri tanımlamaktadır. Bu MCS protokolü, hücrenin sınıflandırılmasına minimum ekipman veya eğitimle ihtiyaç duyulan herhangi bir deneyde izolasyonun kullanılmasını sağlar. Bu teknikler, hücre yüzeyi markörlerinin varlığına dayanan spesifik hücre popülasyonlarının işlevselliğini belirlemek için ileri deneylere yardımcı olabilir.

Preadipositlerin proliferatif kapasitesi ve adiposit öncüllerinin adipojenik potansiyeli, in vitro 11&#39;de muhafaza edilen özelliklerdir. İzole SVF&#39;nin ve APC&#39;nin, erkek sıçanlardan aPVAT, mPVAT ve GON&#39;dan in vitro proliferasyonu, kantitatif bir DNA testi kullanılarak kaplamadan 8, 24, 48 ve 96 saat sonra değerlendirildi. Aynı bölgedeki SVF hücrelerine kıyasla 96 saat daha az proliferasyona sahip olan bir PVA&#3...

Watch this Scientific Journal Video about Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting at JoVE.com

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Burada Manyetik Aktif Hücre Ayırma (MCS) kullanarak Perivasküler Adipoz Doku (PVAT) Adiposit Progenitör Hücre (APC) popülasyonlarının izolasyonu için bir yöntem rapor. Bu yöntem, floresan-Aktive Hücre Ayırma (FACS) ile karşılaştırıldığında, yağ dokusunun gramı başına APC&#39;nin artan bir izolasyonuna izin verir.

Manyetik Aktive Hücre Ayırma ile İzole Edilen Perivasküler Adipoz Doku&#39;ndan Adiposit Progenitörlerinin Genleşmesi ve Adipogenez İndüksiyonu

Expansion of Perivascular Adipose Tissue (PVAT), a major regulator of vascular function through paracrine signaling, is directly related to the ...

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Developmental Biology

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Domuz Subkutan yağ dokularındaki İzolasyonu ve Yağ-Türetilmiş Kök Hücre Farklılaşması

Obesity is an unconstrained worldwide epidemic. Unraveling molecular controls in adipose tissue development holds promise to treat obesity or diabetes. ...

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

İnsan Plasenta Doku türetilen İzolasyon ve mezenkimal kök Genişleme / Stromal hücreler

Mesenchymal stem/stromal cells (MSC) are promising candidates for use in cell-based therapies. In most cases, therapeutic response appears to be cell-dose ...

Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue

İnsan Kardiyak Doku perivasküler Multipotent Öncü hücre popülasyonlarının izolasyonu

Multipotent mesenchymal stem/stromal cells (MSC) were conventionally isolated, through their plastic adherence, from primary tissue digests whilst their ...

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Cardiotoxin Enjeksiyonu ile Akut İskelet Kası Regeneration indüksiyon

Skeletal muscle regeneration is a physiological process that occurs in adult skeletal muscles in response to injury or disease. Acute injury-induced ...

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Santrifüj Yerçekimi ile adipoz kaynaklı Kök Hücrelerin kondrojenik Farklılaşma İndüksiyon

Impaired cartilage cannot heal naturally. Currently, the most advanced therapy for defects in cartilage is the transplantation of chondrocytes ...

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Akım Sitometresi ile Fare Embriyosunda Eritromiyeloid Progenitörlerin Ve Döllerinin Belirlenmesi

Macrophages are professional phagocytes from the innate arm of the immune system. In steady-state, sessile macrophages are found in adult tissues where ...

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

Epididimden İzole Edilmiş Primer Epitelyal Hücrelerin Tüm Hücreli Yama-Kelepçesi Kayıtları

The epididymis is an essential organ for sperm maturation and reproductive health. The epididymal epithelium consists of intricately connected cell types ...

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

İskele-free, üç boyutlu insülin ifade Pancreatoids fare pankreas ataları Vitro gelen nesil

The pancreas is a complex organ composed of many different cell types that work together to regulate blood glucose homeostasis and digestion. These cell ...

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Verimli üretimi, pankreas/oniki parmak bağırsağı Homeobox Protein 1+ Posterior Foregut/pankreas ataları hPSCs yapışma kültürlerde üzerinden

Human pluripotent stem cell (hPSC)-derived pancreatic cells are a promising cell source for regenerative medicine and a platform to study human ...

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Periaortik Yağ Dokusundan Nöral Adipoz-Türetilmiş Kök Hücrelerin İzolasyon, Kültür ve Adipojenik İndüksiyonu

An excessive amount of adipose tissue surrounding the blood vessels (perivascular adipose tissue, also known as PVAT) is associated with a high risk of ...