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DOI :
10.3791/55822-v
May 19th, 2017
Chapters
0:05
Title
11:32
Conclusion
10:14
Results: In Situ Kd of CatchER+ in C2C12 Myoblast Cells
1:30
Transfection of HEK293 Cells
2:43
Transfection of C2C12 Myoblast Cells
4:10
Preparation of Slide and Fluorescence Microscope
6:54
Imaging Drug-induced Ca2+ Transients and In Situ Kd Calibration
我々は、リアルタイム蛍光顕微鏡法を用いて、HEK293および骨格筋C2C12細胞の小胞/筋小胞体(ER / SR)における急速なカルシウム過渡状態をモニタリングするための、標的遺伝子操作されたカルシウムインジケータ(GECI)CatchER + in situ K d測定および較正のためのプロトコルについても議論する。
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