Name: neurobehavioral assessments in a mouse model of neonatal hypoxic-ischemic brain injury
Uploaded: 2017-11-24T12:30:00
Description: Watch this Scientific Journal Video about Neurobehavioral Assessments in a Mouse Model of Neonatal Hypoxic-ischemic Brain Injury at JoVE.com

这项研究得到了国家研究基金会 (NRF-2014R1A2A1A11052042; 2015M3A9B4067068)、大韩民国科学技术部、韩国健康技术研发 & 开发项目 (HI16C1012)、卫生部 & 的资助。福利, 大韩民国, 和世大学医学院的 "Dongwha" 教职员研究援助计划 (6-2016-0126)。

所有的动物都被安置在一个标准的笼子里 (27 x 22.5 x 14 厘米3) 在实验室动物保育协会 (AAALAC) 的认证和给予食物和水ad 随意在交替 12-h 光/暗周期.作者遵循动物保护条例, 实验程序由世大学医学院 (IACUC No. 2010-0252; 2013-0220) 机构动物保育和使用委员会批准。
1. 小鼠新生儿脑损伤模型
<ol><li>用异氟醚麻醉幼崽。<ol>
<li>将幼崽 (少于 5) 放入麻醉盒中, 并关上盖子。</li>
...

<ol>
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在这项研究中, 我们诱发 HI 脑损伤的新生儿 P7-10 CD-1 小鼠, 并确定了脑损害的相关认知和运动缺陷。在这个过程中, 闭塞的单侧右颈动脉是至关重要的。在这一步, 动脉可能被破坏和撕裂。大多数经历过动脉撕裂的幼崽都死了。反之, 如果研究人员结扎了另一条血管而不是单侧右颈动脉, 小狗的大脑只轻度受损, 没有明显的表型可观察到24。
在这项研究中, 由于?...

新生儿 HI 脑损伤发生在幼儿期 (约两个病人每1000儿童)1,2,3,4,5。关于新生儿 hi 脑损伤的研究是重要的, 利用建立的新生儿脑损伤小鼠模型可以促进脑损伤的临床前期研究. 
传统的 HI 模型用于成年大鼠6。对于新生儿模型, Vannucci 法是常用的 P7 大鼠7,8。然而, 因为老鼠和老鼠是略有不同的9,10, 即使它们都是啮齿类动物, 我们在 P7-10 上对 CD-1 幼崽进行了改良的大米 Vannucci 方法, 根据先前的研究表明 P7-10 是未成熟的突, 对应于人类术语 P011,12。通过单侧颈动脉结扎和小鼠对 P7-10 幼犬缺氧8% 氧的暴露, 建立新生儿 HI 小鼠模型。
受手术影响的小鼠在右半球的后外侧区域显示出不同程度的脑损伤。为了识别认知和运动缺陷, 进行了基于 PAT、阶梯行走试验和握力测试的神经行为评估。分析了手术 (即正常) 和 HI 小鼠之间的差异。这项工作提供了关于 HI 鼠标模型的基本信息。MRI 图像显示不同的表型, 根据脑损害的严重性, 使用运动和认知测试分开。

<table border="0" cellpadding="0" cellspacing="0" width="1008">
	<tbody>
		<tr height="25">
			<td height="25" style="height:25px;width:363px;">Hypoxic chamber</td>
			<td style="width:203px;">Jeung Do Bio &#38; Plant Co</td>
			<td colspan="2" style="width:443px;">Experimental Builder&#160;</td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">PAT apparatus&#160;</td>
			<td>Jeung Do Bio &#38; Plant Co</td>
			<td colspan="2">Experimental Builder&#160;</td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">The ladder rung walking&#160;</td>
			<td>Jeung Do Bio &#38; Plant Co</td>
			<td colspan="2">Experimental Builder&#160;</td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">SDI Grip Strength System&#160;</td>
			<td colspan="2">San Diego Instruments Inc.</td>
			<td></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;width:363px;">Grip-Strength Meter</td>
			<td>Ugo Basile&#160;</td>
			<td align="right">47200</td>
			<td></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">Harvard Apparatus Fluovac anesthetizing system&#160;</td>
			<td>Harvard Apparatus</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">Anesthetizing box</td>
			<td></td>
			<td></td>
			<td>acryl box</td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">I-Fran Liquid (Isofluorane)</td>
			<td>Hana Pharm. Co., Ltd.</td>
			<td></td>
			<td>General Anesthetics ( isoflurane 100ml)</td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">CD-1 mice</td>
			<td>Orient Co., Ltd.</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">Blue Nylon Mono Non-Absorbbable suture 5-0 50cm</td>
			<td>Ailee Co., Ltd.</td>
			<td>NB 521</td>
			<td></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">IBM SPSS Statistics</td>
			<td>IBM</td>
			<td>Ver. 23</td>
			<td></td>
		</tr>
	</tbody>
</table>

neurobehavioral assessments in a mouse model of neonatal hypoxic-ischemic brain injury

我们对 CD-1 小鼠进行了单侧颈动脉闭塞, 以建立新生儿缺氧缺血 (hi) 模型, 并通过研究这些小鼠的神经行为功能与手术 (即:正常) 小鼠。在这项研究中, Vannucci 的方法被用来诱导新生儿脑损伤在产后7-10 天 (P7-10) 小鼠。通过单侧颈动脉结扎和暴露于缺氧 (8% O2和 92% N2为 90 min) 对幼犬进行 HI 操作。手术后一周, 受损的大脑用肉眼通过半透明的头骨进行评估, 并根据缺席 ("无皮质损伤" 组) 或皮质损伤的存在 ("皮质损伤" 组) 进行分组。比如右半球的病灶在6周, 进行了以下神经行为测试, 以评估认知和运动功能: 被动回避任务 (PAT), 阶梯行走试验和握力测试。这些行为测试有助于确定新生儿 HI 脑损伤的影响, 并用于其他神经退行性疾病的小鼠模型。在这项研究中, 新生的 HI 脑损伤小鼠显示出与右半球损伤相对应的运动缺陷。行为测试结果与人类新生儿脑瘫或新生儿中风患者出现的缺陷有关。在这项研究中, 建立了一个小鼠脑损伤模型, 并显示了不同程度的运动缺陷和认知障碍相比, 手术的老鼠。这项工作提供了关于 HI 鼠标模型的基本信息。MRI 图像显示不同的表型, 根据严重的大脑损伤的运动和认知测试分开。

所有数据都被表示为平均±标准误差 (SEM)。通过对 SPSS 统计软件进行独立或配对的t测试, 对两个组之间的变量进行比较。p值 < 0.05 被认为具有统计学意义。
新生儿 HI 损伤的大脑表现出不同程度的损伤, 并据此分类 (图 1C-E)。大脑是在14周获得的, 病变是可视化的。图 1C显...

Watch this Scientific Journal Video about Neurobehavioral Assessments in a Mouse Model of Neonatal Hypoxic-ischemic Brain Injury at JoVE.com

Neurobehavioral Assessments in a Mouse Model of Neonatal Hypoxic-ischemic Brain Injury

我们进行了单侧颈动脉闭塞产后 7-10 CD-1 小鼠幼崽创建一个新生儿缺氧缺血 (hi) 模型和调查的影响, hi 脑损伤。我们研究了这些小鼠的神经行为功能与手术正常小鼠相比。

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