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DOI :
10.3791/55842-v
May 15th, 2017
Chapters
0:05
Title
6:11
Results: Single Cell Analysis Reveals Changes in Intracellular Localization of YFP-CheW During V. parahaemolyticus Differentiation
1:14
Induction of Swarmer Cell Differentiation
7:41
Conclusion
3:39
Preparation of V. parahaemolyticus Swarmer Cells for Fluorescence Microscopy
该协议允许单细胞显微镜检查差异不同的副溶血弧菌游泳运动员和swarmer细胞。该方法产生容易获得单细胞分析的swarmer细胞群,并涵盖细胞培养物的制备,诱导分子分化,样品制备和图像分析。
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