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DOI :
10.3791/55842-v
May 15th, 2017
Chapters
0:05
Title
6:11
Results: Single Cell Analysis Reveals Changes in Intracellular Localization of YFP-CheW During V. parahaemolyticus Differentiation
1:14
Induction of Swarmer Cell Differentiation
7:41
Conclusion
3:39
Preparation of V. parahaemolyticus Swarmer Cells for Fluorescence Microscopy
このプロトコールは、差異的に異なる腸炎ビブリオ(Vibrio parahaemolyticus)スイマーおよびスマーマー細胞の単一細胞顕微鏡検査を可能にする。この方法は、単細胞分析のために容易に入手可能なスマーマー細胞の集団を産生し、細胞培養の調製、スマーマー分化の誘導、試料調製および画像分析を包含する。
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