Name: tuning in the hippocampal theta band in vitro: methodologies for recording from the isolated rodent septohippocampal circuit
Uploaded: 2017-08-02T13:00:00
Description: Watch this Scientific Journal Video about Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit at JoVE.com

이 연구는 캐나다 보건 연구소 (Canadian Institute of Health Research and Natural Sciences)의 지원을 받았다.

모든 절차는 McGill 대학 동물 관리위원회 및 동물 관리에 관한 캐나다위원회에서 승인 한 지침 및 지침에 따라 수행되었습니다. 1. 급성 해마 in vitro 준비 참고 : 손상되지 않은 해마 준비를 분리하는 것은 (1) 솔루션 및 장비 준비, (2) 해마 해부 및 (3) 본질적인 세타 진동 생성에 필요한 빠른 관류 속도 시스템 설정. 이 프로토콜에서 해부에서 기록에 ?...

<ol>
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H., Halasy, K., Paulsen, O., Somogyi, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synchronization+of+neuronal+activity+in+hippocampus+by+individual+GABAergic+interneurons.">Synchronization of neuronal activity in hippocampus by individual GABAergic interneurons.</a> Nature. 378 (6552), 75-78 (1995).</li><li>Williams, J. H., Kauer, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Properties+of+carbachol-induced+oscillatory+activity+in+rat+hippocampus.">Properties of carbachol-induced oscillatory activity in rat hippocampus.</a> J Neurophysiol. 78 (5), 2631-2640 (1997).</li><li>Chapman, C. A., Lacaille, J. 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J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+model+of+atropine-resistant+theta+oscillations+in+rat+hippocampal+area+CA1.">A model of atropine-resistant theta oscillations in rat hippocampal area CA1.</a> J Physiol. 543 (Pt 3), 779-793 (2002).</li><li>Gloveli, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthogonal+arrangement+of+rhythm-generating+microcircuits+in+the+hippocampus.">Orthogonal arrangement of rhythm-generating microcircuits in the hippocampus.</a> Proc Natl Acad Sci U S A. 102 (37), 13295-13300 (2005).</li><li>Konopacki, J., Eckersdorf, B., Kowalczyk, T., Golebiewski, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Firing+cell+repertoire+during+carbachol-induced+theta+rhythm+in+rat+hippocampal+formation+slices.">Firing cell repertoire during carbachol-induced theta rhythm in rat hippocampal formation slices.</a> Eur J Neurosci. 23 (7), 1811-1818 (2006).</li><li>Hajos, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Maintaining+network+activity+in+submerged+hippocampal+slices:+importance+of+oxygen+supply.">Maintaining network activity in submerged hippocampal slices: importance of oxygen supply.</a> Eur J Neurosci. 29 (2), 319-327 (2009).</li><li>Manseau, F., Goutagny, R., Danik, M., Williams, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+hippocamposeptal+pathway+generates+rhythmic+firing+of+GABAergic+neurons+in+the+medial+septum+and+diagonal+bands:+an+investigation+using+a+complete+septohippocampal+preparation+in+vitro.">The hippocamposeptal pathway generates rhythmic firing of GABAergic neurons in the medial septum and diagonal bands: an investigation using a complete septohippocampal preparation in vitro.</a> J Neurosci. 28 (15), 4096-4107 (2008).</li><li>Goutagny, R., Jackson, J., Williams, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Self-generated+theta+oscillations+in+the+hippocampus.">Self-generated theta oscillations in the hippocampus.</a> Nat Neurosci. 12 (12), 1491-1493 (2009).</li><li>Jackson, J., Goutagny, R., Williams, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fast+and+slow+gamma+rhythms+are+intrinsically+and+independently+generated+in+the+subiculum.">Fast and slow gamma rhythms are intrinsically and independently generated in the subiculum.</a> J Neurosci. 31 (34), 12104-12117 (2011).</li><li>Amilhon, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parvalbumin+Interneurons+of+Hippocampus+Tune+Population+Activity+at+Theta+Frequency.">Parvalbumin Interneurons of Hippocampus Tune Population Activity at Theta Frequency.</a> Neuron. 86 (5), 1277-1289 (2015).</li><li>Huh, C. 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급성 hippocampal 조각에서 electrophysiological 녹음은 표준 체외 기술을 구성하는 동안, 여기에 제시된 방법은 고전적인 접근 방식과 크게 다릅니다. 특정 세포 층이 표면에서 볼 수 있고 직접 검사 할 수있는 얇은 슬라이스 준비와 달리 손상되지 않은 해마 준비물은 개별 층을 통과하면서 전극을 대상 뇌 영역으로 낮추는 생체 내 구성과 유사합니다. 해마의 무결성은 기능적 연결성 및 국?...

Hippocampal theta oscillations (4 - 12 Hz)는 포유 동물의 뇌에서 가장 두드러진 리듬 활동 중 하나이며, 시공간 정보의 처리 및 일란성 기억 1 , 2 , 3의 형성과 같은인지 기능에서 중요한 역할을한다고 여겨집니다. 공간 탐색 및 병변 연구와 함께 theta-modulated place-cell의 관계를 강조하는 몇 가지 생체 내 연구와 임상 증거는 hippocampal theta oscillations이 기억 형성 4 , 5 , 6에 관여한다는 견해를 뒷받침한다. 해마 생성의 세타 진동은 아직 완전히 이해되지 않았다. 초기 생체 내 조사는 세타 활성이 외인성 발진기, 특히 리듬 입력에 주로 의존한다고 제안했다중격과 entorhinal 피질 같은 구 심성 뇌 구조에서 7 , 8 , 9 , 10 . 내인성 요인 - 해마 신경 세포의 내부 연결성과 해마 뉴런의 성질 -은 시험관 내 관찰 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18에 근거하여 가정되었다. 그러나 몇 가지 획기적인 연구 19 , 20 , 21 을 제외하고 간단한 체외 조각 준비에서 생리적으로 현실적인 인구 활동을 복제 할 수있는 접근법을 개발하는 데 어려움이 있습니다오랫동안 해마와 그 주변의 본질적인 능력을 세타 진동을 스스로 발생시키는 데 더 상세한 실험적 검사를 지연시켰다. 체외의 얇은 슬라이스 실험 환경 에서 표준의 중요한 단점은 뇌 구조의 3D 세포 및 시냅스 조직이 일반적으로 손상된다는 것입니다. 즉, 공간적으로 분산 된 세포 어셈블리를 기반으로하는 여러 형태의 협동 네트워크 활동이 하나 이상의 뇌 영역 (&gt; 1mm)에 걸쳐있는 국소 그룹 (반경 ≤1mm)에서 뉴런 인구에 이르기까지 지원 될 수 없습니다. 이러한 고려 사항을 감안할 때, 세타 진동이 해마에서 어떻게 나타나고 관련 피질 및 피질 하부 구조로 전파되는지 연구하는 데는 다른 유형의 접근법이 필요했습니다. 최근 몇 년 동안 양방향 intera를 검사하기위한 &quot;완전한 septo-hippocampal&quot;준비의 초기 개발두 구조물 (22) 및 「격리 해마 &quot;제제 계속되는 진화 ctions는 고유 진동 세타 리듬 외부 입력 (23)이 결여 된 해마에서 자발적으로 발생하는 것으로 밝혀졌다. 이러한 접근법의 값은 이들 영역의 전체 기능 구성이 체외 (22)에서의 세타 리듬 발전기로서 작동하기 위해 유지했다 초기 통찰력에 놓여있다.

<table border="0" cellpadding="0" cellspacing="0" width="820">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:319px;">Reagents</td>
			<td style="width:135px;"></td>
			<td style="width:119px;"></td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Sodium Chloride</td>
			<td>Sigma Aldrich</td>
			<td>S9625</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Sucrose</td>
			<td>Sigma Aldrich</td>
			<td>S9378</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Sodium Bicarbonate</td>
			<td>Sigma Aldrich</td>
			<td>S5761</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">NaH2PO4 - sodium phosphate monobasic</td>
			<td>Sigma Aldrich</td>
			<td>S8282</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Magnesium sulfate</td>
			<td>Sigma Aldrich</td>
			<td>M7506</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Potassium Chloride</td>
			<td>Sigma Aldrich</td>
			<td>P3911</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">D-(+)-Glucose</td>
			<td>Sigma Aldrich</td>
			<td>G7528</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Calcium chloride dihydrate</td>
			<td>Sigma Aldrich</td>
			<td>C5080</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Sodium Ascorbate</td>
			<td>Sigma Aldrich</td>
			<td>A7631-25G</td>
			<td></td>
		</tr>
		<tr height="30">
			<td height="30" style="height:30px;width:319px;">Name</td>
			<td style="width:135px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Equipment</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Standard Dissecting Scissors</td>
			<td>Fisher Scientific</td>
			<td>08-951-25</td>
			<td>brain extraction</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Scalpel Handle #4, 14cm</td>
			<td>WPI</td>
			<td>500237</td>
			<td>brain extraction</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Filter forceps, flat jaws, straight (11cm)</td>
			<td>WPI</td>
			<td>500456</td>
			<td>brain extraction</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Paragon Stainless Steel Scalpel Blades #20</td>
			<td>Ultident</td>
			<td>02-90010-20</td>
			<td>brain extraction</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Fine Point Curved Dissecting Scissors</td>
			<td>Thermo Fisher Scientific</td>
			<td>711999</td>
			<td>brain extraction</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Teflon (PTFE) -coated thin spatula</td>
			<td>VWR</td>
			<td>82027-534</td>
			<td>hippocampal preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hayman Style Microspatula</td>
			<td>Fisher Scientific</td>
			<td>21-401-25A</td>
			<td>hippocampal preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Lab spoon</td>
			<td>Fisher Scientific</td>
			<td>14-375-20</td>
			<td>hippocampal preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Borosilicate Glass Pasteur Pipets</td>
			<td>Fisher Scientific</td>
			<td>13-678-20A</td>
			<td>hippocampal preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Droper</td>
			<td>Fisher Scientific</td>
			<td></td>
			<td>hippocampal preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Razor blades Single edged</td>
			<td>VWR</td>
			<td>55411-055</td>
			<td>hippocampal preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Lens paper (4X6 inch)</td>
			<td>VWR</td>
			<td>52846-001</td>
			<td>hippocampal preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Glass petri dishes (100 x 20 mm)</td>
			<td>VWR</td>
			<td>25354-080</td>
			<td>hippocampal preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Plastic tray for ice; size 30 x 20 x 5 cm</td>
			<td>n.a.</td>
			<td>n.a.</td>
			<td>hippocampal preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Single Inline Solution Heater</td>
			<td>Warner Instruments</td>
			<td>SH-27B</td>
			<td>perfusion system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Aquarium air stones for bubbling</td>
			<td>n.a.</td>
			<td>n.a.</td>
			<td>perfusion system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Tygon E-3603 tubing (ID 1/16 OD 1/8)</td>
			<td>Fisherbrand</td>
			<td>14-171-129</td>
			<td>perfusion system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Electric Skillet</td>
			<td>Black &#38; Decker</td>
			<td>n.a.</td>
			<td>perfusion system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">95% O2/5% CO2 gas mixture (carbogen)&#160;</td>
			<td>Vitalaire</td>
			<td>SG466204A</td>
			<td>perfusion system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Glass bottles/flasks (4 x 1 L)</td>
			<td>n.a.</td>
			<td>n.a.</td>
			<td>perfusion system</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Submerged recording Chamber</td>
			<td>custom design (FM)</td>
			<td>n.a.</td>
			<td style="width:247px;">Commercial alternative may be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Glass pipettes (1.5 / 0.84 OD/ID (mm) )</td>
			<td>WPI</td>
			<td>1B150F-4</td>
			<td>electrophysiology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hum Bug 50/60 Hz Noise Eliminator</td>
			<td>Quest Scientific</td>
			<td>Q-Humbug</td>
			<td>electrophysiology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Multiclamp 700B patch-clamp amplifier</td>
			<td>Molecular devices</td>
			<td>MULTICLAMP</td>
			<td>electrophysiology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Multiclamp 700B Commander Program</td>
			<td>Molecular devices</td>
			<td>MULTICLAMP</td>
			<td>electrophysiology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Digital/Analogue converter</td>
			<td>Molecular devices</td>
			<td>DDI440</td>
			<td>electrophysiology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">PCLAMP10</td>
			<td>Molecular devices</td>
			<td>PCLAMP10</td>
			<td>electrophysiology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Vibration isolation table&#160;</td>
			<td>Newport</td>
			<td>n.a.</td>
			<td>electrophysiology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micromanipulators (manually operated )</td>
			<td>Siskiyou&#160;</td>
			<td>MX130</td>
			<td>electrophysiology (LFP)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micromanipulators (automated)</td>
			<td>Siskiyou&#160;</td>
			<td>MC1000e</td>
			<td>electrophysiology (patch)</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Audio monitor&#160;</td>
			<td>A-M Systems</td>
			<td>Model 3300</td>
			<td>electrophysiology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micropipette/Patch pipette puller</td>
			<td>Sutter</td>
			<td>P-97</td>
			<td>electrophysiology</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Custom-built upright fluorescence microscope</td>
			<td>Siskiyou</td>
			<td>n.a.</td>
			<td>Imaging</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Analogue video camera</td>
			<td>COHU</td>
			<td>4912-2000/0000</td>
			<td>Imaging</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Digital frame grabber with imaging software</td>
			<td>EPIX, Inc</td>
			<td>PIXCI-SV7</td>
			<td>Imaging</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Olympus 2.5x objective</td>
			<td>Olympus</td>
			<td>MPLFLN</td>
			<td>Imaging</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Olympus 40x water immersion objective</td>
			<td>Olympus</td>
			<td>UIS2 LUMPLFLN</td>
			<td>Imaging</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Custom-made light-emitting diode (LED) system&#160;</td>
			<td>custom</td>
			<td>n.a.</td>
			<td>optogenetic stimulation (Amhilon et al., 2015)</td>
		</tr>
		<tr height="30">
			<td height="30" style="height:30px;width:319px;">Name</td>
			<td style="width:135px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Animals</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:319px;">PV::Cre (KI) mice</td>
			<td style="width:135px;">Jackson Laboratory</td>
			<td style="width:119px;">stock number 008069</td>
			<td style="width:247px;">Allow&#160; Cre-directed gene expression in PV interneurons</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:319px;">Constitutive-conditional Ai9 mice (R26-lox-stop-lox-tdTomato (KI))</td>
			<td style="width:135px;">Jackson Laboratory</td>
			<td style="width:119px;">stock number 007905</td>
			<td style="width:247px;">Express TdTomato following Cre-mediated recombination</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:319px;">Ai32 mice (R26-lox-stop-lox-ChR2(H134R)-EYFP</td>
			<td style="width:135px;">Jackson Laboratory</td>
			<td style="width:119px;">stock 
			number 012569</td>
			<td style="width:247px;">Express the improved channelrhodopsin-2/EYFP fusion protein following exposure to Cre recombinase</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">PVChY mice</td>
			<td>In house breeding</td>
			<td>n.a.</td>
			<td style="width:247px;">Offspring obtained from cross-breeding the PV-Cre line with Ai32 mice (R26-lox-stop-lox-ChR2(H134R)-EYFP</td>
		</tr>
	</tbody>
</table>

tuning in the hippocampal theta band in vitro: methodologies for recording from the isolated rodent septohippocampal circuit

이 프로토콜은 세타 진동의 연구를위한 방법론 및 응용 프로그램의 최근 개선과 함께 고립 된 전체 해마, WT 및 유전자 변형 생쥐에서 준비하고 녹음 절차를 설명합니다. 고립 해마 준비의 간단한 특성 분석을 통해 내부 hippocampal theta 발진기와의 관계를 cornum ammonis-1 (CA1) 및 subiculum (SUB) 영역의 피라미드 세포 및 GABA 신경계 인터페론의 활성과 함께 검사합니다. 전반적으로, 우리는 고립 된 해마가 체외에서 본질적인 세타 진동 을 생성 할 수 있으며, 해마 내에 생성 된 리듬이 parvalbumin 양성 (PV) 신경 세포의 광학 유전 자극에 의해 정확하게 조작 될 수 있음을 보여줍니다. 시험관에서 분리 된 해마 제제는 시각적으로 확인 된 neu에서 동시에 현장 및 세포 내 패치 클램프 기록을 사용할 수있는 독특한 기회를 제공합니다theta 리듬 세대의 기본 메커니즘을 더 잘 이해할 수 있습니다.

<html> 이 섹션 은 체외에서 마우스 격리 hippocampal 준비 에 세타 진동을 공부하여 얻을 수있는 결과의 예를 보여줍니다. 분리 된 해마를 추출하기위한 해부 절차는 그림 1에 설명되어 있습니다. 이 준비를 사용하여 내장 된 세타 진동은 여러 필드 전극을 배치하는 동안 검사 할 수 있으며 전반적인 활동을 기록하고 격리 된 해마의 여러 ​?...

Watch this Scientific Journal Video about Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit at JoVE.com

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

여기에서, 우리는 격리 된 전체 해마 제조물로부터 리듬 뉴런 네트워크 세타 및 감마 진동을 기록하기위한 프로토콜을 제시한다. 우리는 해마 추출에서 현장, 단일 및 전체 세포 패치 클램프 녹음뿐만 아니라 theta 리듬의 광 발생기 페이싱에 이르기까지의 실험 단계를 설명합니다.

Hippocampal 쎄타 밴드의 튜닝<em&gt; 체외에서</em&gt; : 격리 된 설치류 Septohippocampal Circuit에서 기록하는 방법론

This protocol outlines the procedures for preparing and recording from the isolated whole hippocampus, of WT and transgenic mice, along with recent ...

Research

JoVE Journal

Neuroscience

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

역행 형광 라벨이 확인 된 신경 세포의 표적 세포 외 단일 단위 기록을 허용<em&gt; 생체 내</em

The overall goal of this method is to record single-unit responses from an identified population of neurons. In vivo electrophysiological recordings from ...

연구

신경과학

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury

가벼운 외상성 뇌 손상 후 Hippocampal 회로 기능의 수선에 관한 조사

Traumatic Brain Injury (TBI) afflicts more than 1.7 million people in the United States each year and even mild TBI can lead to persistent neurological ...

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

급성 Hippocampal 슬라이스에 Synaptically-evoked Astroglial와 Neuronal 응답의 듀얼 Electrophysiological 레코딩

Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs ...

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits

메뚜기에 신경 활동을 특성화하기 위해 다중 단위 기록 방법 (<em&gt; Schistocerca 아메리카나</em&gt;) 강한 회로

Detection and interpretation of olfactory cues are critical for the survival  of many organisms. Remarkably, species across phyla have strikingly similar ...

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

설치류 중추 신경계에 약물을 직접 심실 배달

Due to an inability to cross the blood brain barrier, certain drugs need to be directly delivered into the central nervous system (CNS). Our lab focuses ...

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

<em&gt; 체외</em성인 마우스 Myenteric 신경총에서 분리 된 장내 뉴런과 교세포의&gt; 준비

The enteric nervous system is a vast network of neurons and glia running the length of the gastrointestinal tract that functionally controls ...

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain

동시 전기 생리 녹화 및 설치류의 뇌에서 억제 에이전트의 마이크로 주사

Here we describe a method for the construction of a single-use &#8220;injectrode&#8221; using commercially accessible and affordable parts. A probing ...

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis

결합 된 방법론을 활용하여 축삭 분기 및 신경 세포 형태 형성 동안 플라즈마 멤브레인 배달의 역할을 정의합니다

During neural development, growing axons extend to multiple synaptic partners by elaborating axonal branches. Axon branching is promoted by extracellular ...

Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

Pedunculopontine 핵 뉴런에서 녹음 감마 밴드 진동

Synaptic efferents from the PPN are known to modulate the neuronal activity of several intralaminar thalamic regions (e.g., the ...

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

급성 Hippocampal 조각에 작은 볼륨 재활용-, 관류-침수 형 챔버 시스템 유지에 시 냅 스가 소성을 기록

Even though experiments on brain slices have been in use since 1951, problems remain that reduce the probability of achieving a stable and successful ...