Results: Assessing PLC-δ1 PH Domain: Phosphoinositide Interaction with PIP-on-a-chip Assay
2:14
Preparing Small Unilamellar Vesicles (SUVs)
3:29
Assembling the Microfluidic Device
4:42
Forming Supported Lipid Bilayers (SLBs)
7:56
Testing PLC-δ1 PH Domain Interction with PI(4,5)P2-containing SLBs
Transcript
The overall goal of the PIP-on-a-chip assay is to assess protein membrane interactions in a quantitative label-free manner. Protein membrane interactions are the heart of so many processes of the cell and its pathogens, but techniques to study the
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Qui presentiamo un bilingue lipidico supportato nel contesto di una piattaforma microfluidica per studiare le interazioni proteine-fosfoinositide usando un metodo senza etichetta basato sulla modulazione del pH.