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DOI :
10.3791/55869-v
July 27th, 2017
Chapters
0:05
Title
1:04
Fabricating Micropatterned PDMS Blocks
9:56
Conclusion
8:49
Results: Assessing PLC-δ1 PH Domain: Phosphoinositide Interaction with PIP-on-a-chip Assay
2:14
Preparing Small Unilamellar Vesicles (SUVs)
3:29
Assembling the Microfluidic Device
4:42
Forming Supported Lipid Bilayers (SLBs)
7:56
Testing PLC-δ1 PH Domain Interction with PI(4,5)P2-containing SLBs
ここでは、pH調節に基づく無標識法を用いてタンパク質 - ホスホイノシチド相互作用を研究するためのマイクロ流体プラットフォームの文脈で、支持された脂質二重層を提示する。
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