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DOI :
10.3791/55881-v
September 9th, 2017
Chapters
0:00
Title
1:25
Immunofluorescence
5:43
Representative Results for Immunofluorescence
6:16
Immunoprecipitation
6:38
Harvesting Cells
7:33
Protein Isolation
8:10
Bradford Assay
8:36
Antibody Coupling of Beads
9:26
Incubation of Total Cell Lysate with Beads
9:52
Elution of Proteins from Beads
10:18
Preparation of Input Controls
10:43
Denaturation of Proteins and Western Blot
13:06
Representative Results for Immunoprecipitation
13:29
Conclusion
ここでは、2 つの抗体を用いたタンパク質間相互作用研究手法を提示: 蛍光抗体法と免役沈降法。これらのテクニックは、蛋白質、細胞のシグナル伝達経路の新規成分の探索と理解蛋白質の原動力の間の物理的な相互作用を研究するために適しています。
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