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DOI :
10.3791/55886-v
July 25th, 2017
Chapters
0:05
Title
0:57
In Utero Electroporation
12:40
Conclusion
10:30
Results: Migration Behavior of Bcl11a Mutant Upper-layer Cortical Neurons
6:40
Vibratome Sectioning and Slice Culture
4:18
Brain Dissection and Embedding for Organotypic Slice Culture
8:47
Ime-lapse Imaging
该方案提供了直接观察径向迁移皮质神经元的指导。 在子宫电穿孔中,组织切片培养和延时共聚焦成像结合直接动态地研究过度表达或下调基因在迁移神经元中的作用并分析其在发育过程中的分化。
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