Name: tubal cytology of the fallopian tube as a promising tool for ovarian cancer early detection
Uploaded: 2017-07-25T13:00:00
Description: Watch this Scientific Journal Video about Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection at JoVE.com

作者要感谢亚利桑那大学病理学系的财政支持。该项目得到了WZ的Mark和Jane Gibson捐赠基金的部分支持。

亚利桑那大学获得了机构审查委员会的批准，用于进行这项研究。获得知情的患者同意书。 患者选择注意：获得了亚利桑那大学的机构审查委员会的批准。共招收38名患者。患者年龄32〜86岁，平均55岁，其中绝经后26例，绝经前12例。 <ol><li>获得每名患者的知情同意书。 </li></ol>输卵管细胞收集程序注意：本研究中包?...

<ol>
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L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Atypical+epithelial+proliferation+in+fallopian+tubes+in+prophylactic+salpingo-oophorectomy+specimens+from+BRCA1+and+BRCA2+germline+mutation+carriers.">Atypical epithelial proliferation in fallopian tubes in prophylactic salpingo-oophorectomy specimens from BRCA1 and BRCA2 germline mutation carriers.</a> Int J Gynecol Pathol. 23 (1), 35-40 (2004).</li><li>Leunen, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prophylactic+salpingo-oophorectomy+in+51+women+with+familial+breast-ovarian+cancer:+importance+of+fallopian+tube+dysplasia.">Prophylactic salpingo-oophorectomy in 51 women with familial breast-ovarian cancer: importance of fallopian tube dysplasia.</a> Int J Gynecol Cancer. 16 (1), 183-188 (2006).</li><li>Piek, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dysplastic+changes+in+prophylactically+removed+Fallopian+tubes+of+women+predisposed+to+developing+ovarian+cancer.">Dysplastic changes in prophylactically removed Fallopian tubes of women predisposed to developing ovarian cancer.</a> J Pathol. 195 (4), 451-456 (2001).</li><li>Crum, C. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+distal+fallopian+tube:+a+new+model+for+pelvic+serous+carcinogenesis.">The distal fallopian tube: a new model for pelvic serous carcinogenesis.</a> Curr Opin Obstet Gynecol. 19 (1), 3-9 (2007).</li><li>Jarboe, E. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tubal+and+ovarian+pathways+to+pelvic+epithelial+cancer:+a+pathological+perspective.">Tubal and ovarian pathways to pelvic epithelial cancer: a pathological perspective.</a> Histopathology. 53 (2), 127-138 (2008).</li><li>Menon, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ovarian+cancer+screening+has+no+effect+on+disease-specific+mortality.">Ovarian cancer screening has no effect on disease-specific mortality.</a> Evid Based Med. 17 (2), 47-48 (2012).</li><li>Buys, S. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+screening+on+ovarian+cancer+mortality:+the+Prostate,+Lung,+Colorectal+and+Ovarian+(PLCO)+Cancer+Screening+Randomized+Controlled+Trial.">Effect of screening on ovarian cancer mortality: the Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Randomized Controlled Trial.</a> JAMA. 305 (22), 2295-2303 (2011).</li><li>Otsuka, I., Kameda, S., Hoshi, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+detection+of+ovarian+and+fallopian+tube+cancer+by+examination+of+cytological+samples+from+the+endometrial+cavity.">Early detection of ovarian and fallopian tube cancer by examination of cytological samples from the endometrial cavity.</a> Br J Cancer. 109 (3), 603-609 (2013).</li><li>Dhanani, M., Nassar, A., Dinh, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Classification+of+Fallopian+Tube+Cytology+Sampling+to+Develop+a+Model+for+Future+Peritoneal+and+Ovarian+Cancer+Screening.">Classification of Fallopian Tube Cytology Sampling to Develop a Model for Future Peritoneal and Ovarian Cancer Screening.</a> J Am Soc Cytopathol. 3 (5), S35-S36 (2014).</li><li>Rodriguez, E. 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已经显示预防性双侧输卵管切除术或输卵管卵巢切除术可降低高危人群发生HGSC的风险，如BRCA基因突变妇女和家族性癌症史。然而，由手术导致的不育和外科绝经是严重后果，造成困难的决定，特别是对于育龄妇女。以前的研究显示输卵管细胞学和组织学之间有很好的相关性17 。我们认为腹腔镜收集的输卵管细胞的输卵管细胞学具有很大的潜力成为早期癌症检测的实用的，微创?...

卵巢高级浆液性癌（HGSC）是最常见和致命的卵巢癌，仍然是对公众健康的主要威胁。在过去十年中，研究人员已经表明，绝大多数的HGSC情况下从该输卵管而不是卵巢本身1，2，3，4，5，6出现。浆液性输卵管上皮内癌（STIC）作为HGSC 1，7，8，9，10，11的前体已被广泛接受。到目前为止，卵巢癌的早期发现仍然很困难。目前的筛选方案与联合癌抗原125（CA-125）和经阴道超声显示对病人的死亡率12，13几乎没有影响。卵巢癌检测的子宫内膜取样显示极低的灵敏度14 。两个先驱研究15,16探索良性输卵管腹腔镜直接采样的使用，并且其特征在于良性输卵管上皮的细胞学特征。我们认为，输卵管的直接取样也可以成为在肿瘤未被成像可视化的早期阶段检测STIC或HGSC的实用且直接的方法。 尽管最终目标是在高风险因素患者中进行微创腹腔镜筛查的实用性，但目前研究的直接目标是：1）建立良性输卵管上皮细胞基线细胞学特征;和2）检测输卵管细胞学检测卵巢癌的敏感性和特异性和癌症前体。因此，我们开发了以下基线输卵管细胞学方法来检测该方案检测HGSC及其前体STIC 17的有效性。在这项研究中，我们利用了大量定期接受的手术标本，除了常规手术程序之外，还对手术切除的输卵管进行了直接取样。 我们最近的研究17显示，恶性肿瘤的恶性或怀疑的细胞学诊断与HGSC的组织学诊断（100％）高度相关。此外，输卵管细胞学评估确定了在本研究中涉及的仅有两个组织学证实的STIC病例的颅内瘤变。我们认为输卵管细胞学在早期检测中具有很大的潜力，将为患者管理提供有价值的信息。

<table>
	<tbody>
		<tr>
			<td>Cytobrush plus GT gentle touch of Medscand sample collection kit</td>
			<td>CooperSurgical</td>
			<td>C0112</td>
			<td></td>
		</tr>
		<tr>
			<td>ThinPrep preservCyt solution</td>
			<td>HOLOGIC</td>
			<td>234005</td>
			<td></td>
		</tr>
		<tr>
			<td>ThinPrep 2000 Processor</td>
			<td>HOLOGIC</td>
			<td>70031-001</td>
			<td></td>
		</tr>
		<tr>
			<td>Papanicolaou Stain, EA 65</td>
			<td>sigma aldrich</td>
			<td>HT40432</td>
			<td></td>
		</tr>
		<tr>
			<td>95% Ethanol</td>
			<td>sigma aldrich</td>
			<td>652261</td>
			<td></td>
		</tr>
		<tr>
			<td>100% Ethanol</td>
			<td>sigma aldrich</td>
			<td>493538</td>
			<td></td>
		</tr>
		<tr>
			<td>Xylene</td>
			<td>sigma aldrich</td>
			<td>214736</td>
			<td></td>
		</tr>
		<tr>
			<td>Hematoxylin</td>
			<td>sigma aldrich</td>
			<td>H9627</td>
			<td></td>
		</tr>
		<tr>
			<td>Sakura Tissue Tek 2000 tissue processor</td>
			<td>SAKURA</td>
			<td>TISSUE-TEK VIP 2000</td>
			<td></td>
		</tr>
		<tr>
			<td>Mayo Dissecting Scissors,5.5 straight</td>
			<td>Pilgrim medical equiment</td>
			<td>FA710-45</td>
			<td></td>
		</tr>
		<tr>
			<td>Chemware Forceps</td>
			<td>United state plastic corp</td>
			<td>76122</td>
			<td></td>
		</tr>
		<tr>
			<td>Tissue-Tek Accu-Edge Trimming blades,short</td>
			<td>SAKURA</td>
			<td>4785</td>
			<td></td>
		</tr>
		<tr>
			<td>Tissue-Tek Accu-Edge Trimming blades handle,short</td>
			<td>SAKURA</td>
			<td>4786</td>
			<td></td>
		</tr>
	</tbody>
</table>

tubal cytology of the fallopian tube as a promising tool for ovarian cancer early detection

目前，广泛接受的是绝大多数卵巢高级浆液性癌（HGSC）来源于输卵管。然而，由于缺乏用于卵巢癌鉴定的标记物或工具，HGSC的早期检测仍然具有挑战性。当肿瘤不可见时，输卵管的直接取样可增强检测肿瘤细胞的敏感性。我们开发了一种从新鲜手术标本直接收集输卵管细胞的方法，与组织学检查结果具有良好的相关性。这种方法为高风险患者群体中微创腹腔镜筛查的未来应用奠定了基础。

我们以前的研究表明，使用温和的触摸刷直接从切除的输卵管取样可以产生定量和定性的足够的标本用于进一步细胞学评估。此外，自动幻灯片制备和修改的Papanicolaou染色的组合使得病理学家能够更好地可视化细胞学细节以做出准确的诊断。 图1中示出了来自良性输卵管的样品的手动快速pap染色的实例。 如图1所示，刷子操纵导...

Watch this Scientific Journal Video about Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection at JoVE.com

Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection

我们探索了输卵管细胞学方法，采用输卵管直接手术切除术作为卵巢癌早期检测的工具。在这里，我们提出一个收集新鲜手术标本的输卵管细胞的方案。

输卵管的管状细胞学作为卵巢癌早期检测的有前景的工具

Currently, it is widely accepted that the vast majority of ovarian high-grade serous carcinoma (HGSC) originate from the fallopian tube. However, due to ...

Research

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Reliable tools for investigating ovarian cancer initiation and progression are urgently needed. While the use of ovarian cancer cell lines remains a ...

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在体内和体外途径研究银河系结构现货卵巢癌转移定植腹膜脂肪

High-grade serous ovarian cancer (HGSC), the cause of widespread peritoneal metastases, continues to have an extremely poor prognosis; fewer than 30% of ...

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model

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评价疗效<em&gt;小时。幽门螺杆菌</em&gt;蛋白HP-NAP为膀胱癌的治疗在原位小鼠模型的治疗工具

Bladder cancer is one of the most common malignancies of the urogenital tract. Intravesical injection of Bacillus Calmette-Gu&#233;rin (BCG) is the gold ...

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Epithelial ovarian cancer (EOC) is the leading cause of death from gynecologic malignancy in the United States.  Mortality is due to diagnosis of 75% of ...

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

造型卵巢癌传播在人类腹腔的器官文化的早期步骤

The pattern of ovarian cancer metastasis is markedly different from that of most other epithelial tumors, because it rarely spreads hematogenously. ...