Name: tubal cytology of the fallopian tube as a promising tool for ovarian cancer early detection
Uploaded: 2017-07-25T13:00:00
Description: Watch this Scientific Journal Video about Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection at JoVE.com

Forfatterne vil gerne anerkende Department of Pathology, University of Arizona, for den økonomiske støtte. Projektet støttes delvist af Mark og Jane Gibson Stiftelsesfond til WZ.

En godkendelse fra institutionen blev godkendt af University of Arizona for at gennemføre denne undersøgelse. Informerede patienters samtykkeformularer blev opnået. 1. Patientvalg BEMÆRK: En godkendelse fra institutionskritisk kontrol blev opnået fra University of Arizona. I alt 38 patienter blev rekrutteret. Patienternes alder varierede fra 32 til 86 år med et gennemsnit på 55 år, hvoraf 26 patienter var postmenopausale og 12 patienter var præ-menopausale...

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G., Kurman, R. J., Ronnett, B. M., Shih Ie, M., Vang, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Are+all+pelvic+(nonuterine)+serous+carcinomas+of+tubal+origin?.">Are all pelvic (nonuterine) serous carcinomas of tubal origin?.</a> Am J Surg Pathol. 34 (10), 1407-1416 (2010).</li><li>Zheng, W., Fadare, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fallopian+tube+as+main+source+for+ovarian+and+pelvic+(non-endometrial)+serous+carcinomas.">Fallopian tube as main source for ovarian and pelvic (non-endometrial) serous carcinomas.</a> Int J Clin Exp Pathol. 5 (3), 182-186 (2012).</li><li>Carcangiu, M. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Atypical+epithelial+proliferation+in+fallopian+tubes+in+prophylactic+salpingo-oophorectomy+specimens+from+BRCA1+and+BRCA2+germline+mutation+carriers.">Atypical epithelial proliferation in fallopian tubes in prophylactic salpingo-oophorectomy specimens from BRCA1 and BRCA2 germline mutation carriers.</a> Int J Gynecol Pathol. 23 (1), 35-40 (2004).</li><li>Leunen, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prophylactic+salpingo-oophorectomy+in+51+women+with+familial+breast-ovarian+cancer:+importance+of+fallopian+tube+dysplasia.">Prophylactic salpingo-oophorectomy in 51 women with familial breast-ovarian cancer: importance of fallopian tube dysplasia.</a> Int J Gynecol Cancer. 16 (1), 183-188 (2006).</li><li>Piek, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dysplastic+changes+in+prophylactically+removed+Fallopian+tubes+of+women+predisposed+to+developing+ovarian+cancer.">Dysplastic changes in prophylactically removed Fallopian tubes of women predisposed to developing ovarian cancer.</a> J Pathol. 195 (4), 451-456 (2001).</li><li>Crum, C. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+distal+fallopian+tube:+a+new+model+for+pelvic+serous+carcinogenesis.">The distal fallopian tube: a new model for pelvic serous carcinogenesis.</a> Curr Opin Obstet Gynecol. 19 (1), 3-9 (2007).</li><li>Jarboe, E. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tubal+and+ovarian+pathways+to+pelvic+epithelial+cancer:+a+pathological+perspective.">Tubal and ovarian pathways to pelvic epithelial cancer: a pathological perspective.</a> Histopathology. 53 (2), 127-138 (2008).</li><li>Menon, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ovarian+cancer+screening+has+no+effect+on+disease-specific+mortality.">Ovarian cancer screening has no effect on disease-specific mortality.</a> Evid Based Med. 17 (2), 47-48 (2012).</li><li>Buys, S. 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Profylaktisk bilateral salpingektomi eller salpingo-oophorektomi har vist sig at mindske risikoen for at udvikle HGSC i højrisikopopulationer, såsom kvinder med BRCA-genmutationer og stærk familiekræfthistorie. Steriliteten og den kirurgiske overgangsalderen forårsaget af operationen er imidlertid alvorlige konsekvenser og gør en vanskelig beslutning, især for kvinder i den fødedygtige alder. Den tidligere undersøgelse har vist en fremragende sammenhæng mellem tubal cytologi og histologi 17</...

Højvarig serøs carcinom (HGSC) er den mest almindelige og dødelige type kræft i æggestokkene og er fortsat en stor trussel for folkesundheden. I det sidste årti har forskere antydet, at langt størstedelen af ​​HGSC-tilfælde stammer fra æggelederne i stedet for æggestokken selv 1 , 2 , 3 , 4 , 5 , 6 . Serøs tubal intraepithelial carcinoma (STIC) er almindeligt accepteret som forløber for HGSC 1 , 7 , 8 , 9 , 10 , 11 . Hidtil er tidlig opdagelse af kræft i æggestokkene stadig vanskelig. Den nuværende screeningsprotokol med kombineret cancer antigen 125 (CA-125) og transvaginal ultralyd har vistLille effekt på patientdødeligheden 12 , 13 . Endometrialprøveudtagning til påvisning af ovariecancer har vist en ekstremt lav følsomhed 14 . To pionerstudier 15 , 16 undersøgte brugen af ​​laparoskopisk direkte prøveudtagning af godartede æggeleder og karakteriserede de cytologiske egenskaber ved godartet tubalepitel. Vi mener, at direkte prøveudtagning fra æggelederne også kan være en praktisk og ligetil måde at påvise STIC eller HGSC på et tidligt stadium, når tumoren ikke visualiseres ved billeddannelse. Selvom det ultimative mål er brugen af ​​minimalt invasiv laparoskopisk screening hos patienter med højrisikofaktorer, er de umiddelbare mål med den nuværende undersøgelse: 1) At fastslå de basale cytologiske egenskaber af godartet tubal epithelia; Og 2) For at teste sensitiviteten og specificiteten af ​​tubal cytologi ved detektion af æggestokkeneErs og kræftprecursorer. Vi udviklede derfor følgende baseline tubal cytology metode til at teste effektiviteten af ​​denne protokol til påvisning af HGSC og dens precursor STIC 17 . I denne undersøgelse udnyttede vi det store antal regelmæssigt modtagne kirurgiske prøver og udførte direkte prøveudtagning af det kirurgisk udskårne æggeledning ud over rutinemæssige bruttoprocedurer. Vores seneste undersøgelse 17 viste, at den cytologiske diagnose af ondartet eller mistanken for malignitet er stærkt korreleret med den histologiske diagnose af HGSC (100%). Desuden identificerede den tubale cytologiske evaluering intratubal neoplasi i de eneste to histologisk bekræftede STIC-tilfælde, der var involveret i denne undersøgelse. Vi mener, at tubal cytologi har stort potentiale ved tidlig påvisning og vil give værdifuld information til patientstyring.

<table>
	<tbody>
		<tr>
			<td>Cytobrush plus GT gentle touch of Medscand sample collection kit</td>
			<td>CooperSurgical</td>
			<td>C0112</td>
			<td></td>
		</tr>
		<tr>
			<td>ThinPrep preservCyt solution</td>
			<td>HOLOGIC</td>
			<td>234005</td>
			<td></td>
		</tr>
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			<td>ThinPrep 2000 Processor</td>
			<td>HOLOGIC</td>
			<td>70031-001</td>
			<td></td>
		</tr>
		<tr>
			<td>Papanicolaou Stain, EA 65</td>
			<td>sigma aldrich</td>
			<td>HT40432</td>
			<td></td>
		</tr>
		<tr>
			<td>95% Ethanol</td>
			<td>sigma aldrich</td>
			<td>652261</td>
			<td></td>
		</tr>
		<tr>
			<td>100% Ethanol</td>
			<td>sigma aldrich</td>
			<td>493538</td>
			<td></td>
		</tr>
		<tr>
			<td>Xylene</td>
			<td>sigma aldrich</td>
			<td>214736</td>
			<td></td>
		</tr>
		<tr>
			<td>Hematoxylin</td>
			<td>sigma aldrich</td>
			<td>H9627</td>
			<td></td>
		</tr>
		<tr>
			<td>Sakura Tissue Tek 2000 tissue processor</td>
			<td>SAKURA</td>
			<td>TISSUE-TEK VIP 2000</td>
			<td></td>
		</tr>
		<tr>
			<td>Mayo Dissecting Scissors,5.5 straight</td>
			<td>Pilgrim medical equiment</td>
			<td>FA710-45</td>
			<td></td>
		</tr>
		<tr>
			<td>Chemware Forceps</td>
			<td>United state plastic corp</td>
			<td>76122</td>
			<td></td>
		</tr>
		<tr>
			<td>Tissue-Tek Accu-Edge Trimming blades,short</td>
			<td>SAKURA</td>
			<td>4785</td>
			<td></td>
		</tr>
		<tr>
			<td>Tissue-Tek Accu-Edge Trimming blades handle,short</td>
			<td>SAKURA</td>
			<td>4786</td>
			<td></td>
		</tr>
	</tbody>
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tubal cytology of the fallopian tube as a promising tool for ovarian cancer early detection

I øjeblikket er det almindeligt anerkendt, at langt størstedelen af ​​æggestokkene af høj kvalitet serøs carcinom (HGSC) stammer fra æggelederen. På grund af manglen på markører eller værktøjer til identifikation af ovariecancer forbliver den tidlige påvisning af HGSC imidlertid udfordrende. Direkte prøveudtagning af fallopierøret kan øge følsomheden til detektion af neoplastiske celler, når tumoren ikke er groft synlig. Vi udviklede en procedure til indsamling af æggelederceller direkte fra nyligt modtagne kirurgiske prøver, hvilket har vist fremragende sammenhæng med histologiske fund. Denne fremgangsmåde udgør grundlaget for det fremtidige anvendelsesområde for minimalt invasiv laparoskopisk screening hos højrisiko patientpopulationer.

Vores tidligere undersøgelse viste, at prøveudtagning direkte fra det udskårne æggeleder ved hjælp af en blid berøringsbørste kunne give kvantitativ og kvalitativt tilstrækkelig prøve til yderligere cytologisk evaluering. Desuden giver kombinationen af ​​automatiseret diasbehandling og modificeret Papanicolaou-farvning patologen mulighed for bedre at visualisere de cytologiske detaljer for at foretage en nøjagtig diagnose. Et eksempel på en manuel quick pap plet af en prø...

Watch this Scientific Journal Video about Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection at JoVE.com

Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection

Vi undersøgte en tubal cytologisk metode ved at prøve stikprøven direkte efter kirurgisk excision som et værktøj til tidlig opdagelse af ovariecancer. Her præsenterer vi en protokol til indsamling af æggelederceller fra nyligt modtagne kirurgiske prøver.

Tubal Cytology of the Fallopian Tube som et lovende værktøj til tidlig opdagelse af ovariecancer

Currently, it is widely accepted that the vast majority of ovarian high-grade serous carcinoma (HGSC) originate from the fallopian tube. However, due to ...

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