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DOI :
10.3791/55924-v
July 13th, 2017
Chapters
0:05
Title
0:55
Preparation of Cryostat Sections
1:59
Fixing Sections and Hybridization
3:30
Washing, Blocking, and Immunohistochemistry
5:10
Staining
7:02
Results: Neuronal Identity of Antennal Cells Expressing LmigORs by Chromogenic and TSA Detections
8:27
Conclusion
この論文では、ジゴキシゲニン(DIG)標識またはビオチン標識プローブを用いた昆虫アンテナにおいて、特に低レベル発現匂い受容体(OR)遺伝子ならびに他の遺伝子の詳細かつ非常に有効なRNA in situハイブリダイゼーションプロトコルについて記載している。
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