Name: recording synaptic plasticity in acute hippocampal slices maintained in a small-volume recycling-, perfusion-, and submersion-type chamber system
Uploaded: 2018-01-01T13:00:00
Description: Watch this Scientific Journal Video about Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System at JoVE.com

트 면 실시, 분석, 실험을 설계 하 고 원고를 썼다. D.X. 및 C.P. 그림 준비에서 원조 하 고 실험을 실시. 이 작품은 NSFC (31320103906)에 의해 지원 되었다 결핵을 111 프로젝트 (B16013)

동물 동물 보호의 설립된 기준 및 뇌 과학 연구소 및 상태 키 실험실의 의학 신경 생물학의 복 단 대학, 상해, 중국의 절차에 따라 유지 되었다.
1. 솔루션 준비
주: 자료의 테이블을 참조 하십시오.
<ol>
	<li>조각화 버퍼 (수정된 Gey 솔루션) 준비: 92 m m NaCl, 2.5 m m KCl, 1.25 m m NaH2포4, 30 mM NaHCO3, 25 mM 포도 당, 20 mM HEPES, 3mm 나+-pyruvate, 10mm MgSO4</sub...

<ol>
	<li>McIlwain, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolic+response+in+vitro+to+electrical+stimulation+of+sections+of+mammalian+brain.">Metabolic response in vitro to electrical stimulation of sections of mammalian brain.</a> Biochem J. 48 (4), (1951).</li><li>McIlwain, H., Richards, C. D., Somerville, A. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responses+in+vitro+from+the+piriform+cortex+of+the+rat,+and+their+susceptibility+to+centrally-acting+drugs.">Responses in vitro from the piriform cortex of the rat, and their susceptibility to centrally-acting drugs.</a> J Neurochem. 14 (9), 937-938 (1967).</li><li>Yamamoto, C., McIlwain, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrical+activities+in+thin+sections+from+the+mammalian+brain+maintained+in+chemically-defined+media+in+vitro.">Electrical activities in thin sections from the mammalian brain maintained in chemically-defined media in vitro.</a> J Neurochem. 13 (12), 1333-1343 (1966).</li><li>Andersen, P., Bliss, T. V., Lomo, T., Olsen, L. I., Skrede, K. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lamellar+organization+of+hippocampal+excitatory+pathways.">Lamellar organization of hippocampal excitatory pathways.</a> Acta Physiol Scand. 76 (1), 4-5 (1969).</li><li>Skrede, K. K., Westgaard, R. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+transverse+hippocampal+slice:+a+well-defined+cortical+structure+maintained+in+vitro.">The transverse hippocampal slice: a well-defined cortical structure maintained in vitro.</a> Brain Res. 35 (2), 589-593 (1971).</li><li>Kirov, S. A., Sorra, K. E., Harris, K. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Slices+have+more+synapses+than+perfusion-fixed+hippocampus+from+both+young+and+mature+rats.">Slices have more synapses than perfusion-fixed hippocampus from both young and mature rats.</a> J Neurosci. 19 (8), 2876-2886 (1999).</li><li>Schwartzkroin, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characteristics+of+CA1+neurons+recorded+intracellularly+in+the+hippocampal+in+vitro+slice+preparation.">Characteristics of CA1 neurons recorded intracellularly in the hippocampal in vitro slice preparation.</a> Brain Res. 85 (3), 423-436 (1975).</li><li>Schwartzkroin, P. A., Wester, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-lasting+facilitation+of+a+synaptic+potential+following+tetanization+in+the+in+vitro+hippocampal+slice.">Long-lasting facilitation of a synaptic potential following tetanization in the in vitro hippocampal slice.</a> Brain Res. 89 (1), 107-119 (1975).</li><li>Reymann, K. G., Frey, J. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+late+maintenance+of+hippocampal+LTP:+requirements,+phases,+'synaptic+tagging',+'late-associativity'+and+implications.">The late maintenance of hippocampal LTP: requirements, phases, 'synaptic tagging', 'late-associativity' and implications.</a> Neuropharm. 52 (1), 24-40 (2007).</li><li>Bliss, T. V., Collingridge, G. L., Morris, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synaptic+plasticity+in+health+and+disease:+introduction+and+overview.">Synaptic plasticity in health and disease: introduction and overview.</a> Philos Trans R Soc Lond B Biol Sci. 369 (1633), 20130129 (2014).</li><li>Bliss, T. V., Gardner-Medwin, A. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-lasting+potentiation+of+synaptic+transmission+in+the+dentate+area+of+the+unanaestetized+rabbit+following+stimulation+of+the+perforant+path.">Long-lasting potentiation of synaptic transmission in the dentate area of the unanaestetized rabbit following stimulation of the perforant path.</a> J Physiol. 232 (2), 357-374 (1973).</li><li>Reid, K. H., Edmonds, H. L., Schurr, A., Tseng, M. T., West, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pitfalls+in+the+Use+of+Brain-Slices.">Pitfalls in the Use of Brain-Slices.</a> Prog Neurobiol. 31 (1), 1-18 (1988).</li><li>Rambani, K., Vukasinovic, J., Glezer, A., Potter, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Culturing+thick+brain+slices:+an+interstitial+3D+microperfusion+system+for+enhanced+viability.">Culturing thick brain slices: an interstitial 3D microperfusion system for enhanced viability.</a> J Neurosci Methods. 180 (2), 243-254 (2009).</li><li>Hajos, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Maintaining+network+activity+in+submerged+hippocampal+slices:+importance+of+oxygen+supply.">Maintaining network activity in submerged hippocampal slices: importance of oxygen supply.</a> Eur J Neurosci. 29 (2), 319-327 (2009).</li><li>Redondo, R. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synaptic+tagging+and+capture:+differential+role+of+distinct+calcium/calmodulin+kinases+in+protein+synthesis-dependent+long-term+potentiation.">Synaptic tagging and capture: differential role of distinct calcium/calmodulin kinases in protein synthesis-dependent long-term potentiation.</a> J Neurosci. 30 (14), 4981-4989 (2010).</li><li>Stopps, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Design+and+application+of+a+novel+brain+slice+system+that+permits+independent+electrophysiological+recordings+from+multiple+slices.">Design and application of a novel brain slice system that permits independent electrophysiological recordings from multiple slices.</a> J Neurosci Methods. 132 (2), 137-148 (2004).</li><li>Behnisch, T., Matsushita, S., Knopfel, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+of+gene+expression+during+long-term+potentiation.">Imaging of gene expression during long-term potentiation.</a> Neuroreport. 15 (13), 2039-2043 (2004).</li><li>Karpova, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Encoding+and+transducing+the+synaptic+or+extrasynaptic+origin+of+NMDA+receptor+signals+to+the+nucleus.">Encoding and transducing the synaptic or extrasynaptic origin of NMDA receptor signals to the nucleus.</a> Cell. 152 (5), 1119-1133 (2013).</li><li>Karpova, A., Mikhaylova, M., Thomas, U., Knopfel, T., Behnisch, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Involvement+of+protein+synthesis+and+degradation+in+long-term+potentiation+of+Schaffer+collateral+CA1+synapses.">Involvement of protein synthesis and degradation in long-term potentiation of Schaffer collateral CA1 synapses.</a> J Neurosci. 26 (18), 4949-4955 (2006).</li><li>Weng, W., Chen, Y., Wang, M., Zhuang, Y., Behnisch, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Potentiation+of+Schaffer-Collateral+CA1+Synaptic+Transmission+by+eEF2K+and+p38+MAPK+Mediated+Mechanisms.">Potentiation of Schaffer-Collateral CA1 Synaptic Transmission by eEF2K and p38 MAPK Mediated Mechanisms.</a> Front Cell Neurosci. 10 (247), (2016).</li><li>Meduna, L. J., Jackman, A. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Carbon+dioxide+inhalation+therapy.">Carbon dioxide inhalation therapy.</a> Res Publ Assoc Res Nerv Ment Dis. 31, 280-286 (1953).</li><li>Edwards, F. A., Konnerth, A., Sakmann, B., Takahashi, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+thin+slice+preparation+for+patch+clamp+recordings+from+neurones+of+the+mammalian+central+nervous+system.">A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system.</a> Pflugers Arch. 414 (5), 600-612 (1989).</li><li>Mathis, D. M., Furman, J. L., Norris, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preparation+of+acute+hippocampal+slices+from+rats+and+transgenic+mice+for+the+study+of+synaptic+alterations+during+aging+and+amyloid+pathology.">Preparation of acute hippocampal slices from rats and transgenic mice for the study of synaptic alterations during aging and amyloid pathology.</a> J Vis Exp. (49), (2011).</li><li>Yuanxiang, P., Bera, S., Karpova, A., Kreutz, M. R., Mikhaylova, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+of+CA1+nuclear+enriched+fractions+from+hippocampal+slices+to+study+activity-dependent+nuclear+import+of+synapto-nuclear+messenger+proteins.">Isolation of CA1 nuclear enriched fractions from hippocampal slices to study activity-dependent nuclear import of synapto-nuclear messenger proteins.</a> J Vis Exp. (90), e51310 (2014).</li><li>Leutgeb, J. K., Frey, J. U., Behnisch, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=LTP+in+cultured+hippocampal-entorhinal+cortex+slices+from+young+adult+(P25-30)+rats.">LTP in cultured hippocampal-entorhinal cortex slices from young adult (P25-30) rats.</a> J Neurosci Meth. 130 (1), 19-32 (2003).</li><li>Kloosterman, F., Peloquin, P., Leung, L. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Apical+and+basal+orthodromic+population+spikes+in+hippocampal+CA1+in+vivo+show+different+origins+and+patterns+of+propagation.">Apical and basal orthodromic population spikes in hippocampal CA1 in vivo show different origins and patterns of propagation.</a> J Neurophysiol. 86 (5), 2435-2444 (2001).</li><li>Thiemann, W., Malisch, R., Reymann, K. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+microcirculation+chamber+for+inexpensive+long-term+investigations+of+nervous+tissue+in+vitro.">A new microcirculation chamber for inexpensive long-term investigations of nervous tissue in vitro.</a> Brain Res Bull. 17 (1), 1-4 (1986).</li><li>Shetty, M. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Investigation+of+Synaptic+Tagging/Capture+and+Cross-capture+using+Acute+Hippocampal+Slices+from+Rodents.">Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents.</a> J Vis Exp. (103), (2015).</li><li>Du, H., Lin, J., Zuercher, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Higher+efficiency+of+CO2+injection+into+seawater+by+a+venturi+than+a+conventional+diffuser+system.">Higher efficiency of CO2 injection into seawater by a venturi than a conventional diffuser system.</a> Bioresour Technol. 107, 131-134 (2012).</li><li>Weinman, J., Mahler, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+Analysis+of+Electrical+Properties+of+Metal+Electrodes.">An Analysis of Electrical Properties of Metal Electrodes.</a> Med Electron Biol Eng. 2, 299-310 (1964).</li><li>Fanselow, M. S., Dong, H. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Are+the+dorsal+and+ventral+hippocampus+functionally+distinct+structures.">Are the dorsal and ventral hippocampus functionally distinct structures.</a> Neuron. 65 (1), 7-19 (2010).</li><li>Wang, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translation+of+BDNF-gene+transcripts+with+short+3'+UTR+in+hippocampal+CA1+neurons+improves+memory+formation+and+enhances+synaptic+plasticity-relevant+signaling+pathways.">Translation of BDNF-gene transcripts with short 3' UTR in hippocampal CA1 neurons improves memory formation and enhances synaptic plasticity-relevant signaling pathways.</a> Neurobiol Learn Mem. , (2016).</li></ol>

인터페이스 슬라이스 챔버 더 강력한 시 냅 스 응답25,26,,2728전시, 침수 챔버 패치 클램프 기록 및 형광에 대 한 추가 편의 제공 이미지입니다. 따라서, 우리 신경17,18, 형광 프로브의 이미지를 쉽게 확장할 수 있는 상업 잠수 슬라이스 챔버를 사용 하 여 ?...

1951 년에 처음 보고 급성 뇌 조각 실험 실시1했다. Piriform 피2,3 , hippocampal 신경 해 마4, 중 septotemporal 축 transversely 상호는 발견에서 성공적인 생체 외에서 녹음 후 1971 년에는 첫 체 외에 녹음 hippocampal 신경 활동의 달성된5이었다. Vivo에서 그리고 생체 외에서 조건 하에서 뉴런의 신경 생리학 또는 neurostructural 매개 변수의 유사성은 여전히6의 일부 토론 주제 하지만 1975 년에, Schwartzkroin7 표시는 기저 뉴런의 속성은 체 외에서 고 hippocampal 형성에 afferents의 그 높은-주파수 자극 (즉, tetanization)의 시 냅 스 전위8오랫동안 촉진 유도. 신경 활동의 기록 electrophysiological 생체 외에서 크게 확장 활동-종속 시 냅 스가 소성9,10, 블 리스에 의해 1973 년에 발견 했다 세포 메커니즘의 연구 외. 11 vivo에서 토끼로 실험.
신호 경로 뇌 조각, 그리고 급성 hippocampal 조각, 특히 신경 활동의 연구는 이제 표준 도구입니다. 그러나, 의외로, 실험 체 외에 는 아직 표준화 될 준비 및 급성 hippocampal 조각의 유지 보수에 대 한 여전히 존재 하는 여러 방법에 의해 입증. 리드 외. (1988) 12 조각 챔버의 다른 유형과 매체, pH, 온도 및 산소 레벨 목욕의 선택에 급성 뇌 조각의 유지 보수에 대 한 방법론 문제 검토. 이러한 매개 변수는 여전히 조각-녹음 설정 생체 외에서 의 맞춤 요소 때문 녹음 실에서 제어 하기 어렵다. 게시는 설명할 수 있습니다. 극복 하는 데 도움이 방법론 도전과 그의 일부 침수 슬라이스 챔버, 중간 3D microperfusion 시스템13, 향상 된 층 류 및 산소 챔버의 새로운 유형을 찾을 수 있습니다. 14, 컴퓨터 온도 제어15, 시스템 및 다중 챔버 녹음 시스템16공급 한다. 이러한 챔버 구축 하기 쉬운 이므로, 대부분의 과학자 들은 상업적으로 사용 가능한 슬라이스 챔버에 의존 합니다. 이 챔버는 전기 생리학 및 형광 이미징17,,1819의 조합에 대 한 되므로 현미경 시스템에 장착할 수 있습니다. 이 약 실은 뇌 조각 인공 척수 (실제)에 빠져들 계속, 버퍼 솔루션의 높은 유량 필요 유지, 약물 응용 프로그램의 비용을 증가 합니다. 이 위해, 우리는 유출-carbogenation 침수 슬라이스 챔버는 상대적으로 작은 실제 볼륨을 사용 하 여 필드 전위의 장기 기록에 대 한 충분 한 안정성을 제공 하는 재활용 관류 시스템을 통합 했습니다. 또한, 우리는이 실험 carbogenation/관류 시스템의 사용 활동-종속 시 냅 스가 소성10 의 결과 미치는 영향 및 진 핵 신장 요인 2 키 니 아 제 (eEF2K)의 억제 시 냅 스 변조 방법 요약 전송20.

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			<td height="21" style="width:311px;height:21px;">Reagents required</td>
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			<td height="27" style="width:311px;height:27px;">NaCl</td>
			<td style="width:244px;">Sinopharm Chemical Reagent, China</td>
			<td style="width:179px;">10019318</td>
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			<td height="27" style="width:311px;height:27px;">KCl</td>
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			<td style="width:179px;">10016318</td>
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			<td height="27" style="width:311px;height:27px;">KH2PO4</td>
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			<td height="27" style="width:311px;height:27px;">MgCl2&#183;6H2O</td>
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			<td style="width:179px;">10012818</td>
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			<td height="27" style="width:311px;height:27px;">CaCl2</td>
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			<td height="27" style="width:311px;height:27px;">Glucose</td>
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		</tr>
		<tr height="27">
			<td height="27" style="width:311px;height:27px;">NaH2PO4</td>
			<td style="width:244px;">Sinopharm Chemical Reagent, China</td>
			<td style="width:179px;">20040718</td>
			<td></td>
		</tr>
		<tr height="27">
			<td height="27" style="width:311px;height:27px;">HEPES</td>
			<td style="width:244px;">Sigma</td>
			<td style="width:179px;">H3375</td>
			<td></td>
		</tr>
		<tr height="27">
			<td height="27" style="width:311px;height:27px;">Sodium pyruvate</td>
			<td style="width:244px;">Sigma</td>
			<td style="width:179px;">A4043</td>
			<td></td>
		</tr>
		<tr height="27">
			<td height="27" style="width:311px;height:27px;">MgSO4</td>
			<td style="width:244px;">Sinopharm Chemical Reagent, China</td>
			<td style="width:179px;">20025118</td>
			<td></td>
		</tr>
		<tr height="27">
			<td height="27" style="width:311px;height:27px;">NaOH</td>
			<td style="width:244px;">Sinopharm Chemical Reagent, China</td>
			<td style="width:179px;">10019718</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;"></td>
			<td style="width:244px;"></td>
			<td style="width:179px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Tools and materials for dissection</td>
			<td style="width:244px;"></td>
			<td style="width:179px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Decapitators</td>
			<td style="width:244px;">Harvard apparatus</td>
			<td style="width:179px;">55-0012</td>
			<td>for rat decapitation</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Bandage Scissors</td>
			<td style="width:244px;">SCHREIBER</td>
			<td style="width:179px;">12-4227</td>
			<td>for mouse decapitation</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">double-edge blade</td>
			<td style="width:244px;">Flying Eagle, China</td>
			<td style="width:179px;">74-C</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">IRIS Scissors</td>
			<td style="width:244px;">RWD, China</td>
			<td style="width:179px;">S12003-09</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Bone Rongeurs</td>
			<td style="width:244px;">RWD, China</td>
			<td style="width:179px;">S22002-14</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Spoon</td>
			<td style="width:244px;">Hammacher&#160;</td>
			<td style="width:179px;">HSN 152-13</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">dental cement spatula</td>
			<td style="width:244px;">Hammacher&#160;</td>
			<td style="width:179px;">HSN 016-15</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">dental double end excavator</td>
			<td style="width:244px;">Blacksmith Surgical, USA</td>
			<td style="width:179px;">BS-415-017</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Vibrating Microtome</td>
			<td style="width:244px;">Leica, Germany</td>
			<td style="width:179px;">VT1200S</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">surgical blade&#160;</td>
			<td style="width:244px;">RWD, China</td>
			<td style="width:179px;">S31023-02</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">surgical holder</td>
			<td style="width:244px;">RWD, China</td>
			<td style="width:179px;">S32007-14</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;"></td>
			<td style="width:244px;"></td>
			<td style="width:179px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="width:311px;height:42px;">Electrophysiology equipment and materials</td>
			<td style="width:244px;"></td>
			<td style="width:179px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Vertical Pipette Puller</td>
			<td style="width:244px;">Narishige, Japan</td>
			<td style="width:179px;">PC-10</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Vibration isolation table</td>
			<td style="width:244px;">Meirits, Japan</td>
			<td style="width:179px;">ADZ-A0806</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">submerged type recording chamber</td>
			<td style="width:244px;">Warner Instruments</td>
			<td style="width:179px;">RC-26GLP</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">thermostatic water bath</td>
			<td style="width:244px;">Zhongcheng Yiqi&#65292;China</td>
			<td style="width:179px;">HH-1</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">4 Axis Micromanipulator</td>
			<td style="width:244px;">Sutter, USA</td>
			<td style="width:179px;">MP-285, MP-225</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Platinum Wire</td>
			<td style="width:244px;">World Precision Instruments</td>
			<td style="width:179px;">PTP406</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Amplifier</td>
			<td style="width:244px;">Molecular Devices, USA</td>
			<td style="width:179px;">Multiclamp 700B</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Data Acquisition System</td>
			<td style="width:244px;">Molecular Devices, USA</td>
			<td style="width:179px;">Digidata 1440A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Anaysis software</td>
			<td style="width:244px;">Molecular Devices, USA</td>
			<td style="width:179px;">Clampex 10.2</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Fluorescence Microscope</td>
			<td style="width:244px;">Nikon, Japan</td>
			<td style="width:179px;">FN1</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">LED light source</td>
			<td style="width:244px;">Lumen Dynamics Group, Canada</td>
			<td style="width:179px;">X-cite 120LED</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">micropipettes</td>
			<td style="width:244px;">Harvard apparatus</td>
			<td style="width:179px;">GC150TF</td>
			<td>extracelluar recording</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">borosilicate micropipettes</td>
			<td style="width:244px;">Sutter, USA</td>
			<td style="width:179px;">BF150-86</td>
			<td>patch clamp</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">tungsten electrode</td>
			<td style="width:244px;">A-M Systems, USA</td>
			<td style="width:179px;">575500</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">peristaltic pump</td>
			<td style="width:244px;">Longer, China</td>
			<td style="width:179px;">BT00-300T</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">tubes for peristaltic pump</td>
			<td style="width:244px;">ISMATEC, Wertheim, Germany</td>
			<td style="width:179px;">SC0309</td>
			<td>1x inflow, ID: 1.02mm</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">tubes for peristaltic pump</td>
			<td style="width:244px;">ISMATEC, Wertheim, Germany</td>
			<td style="width:179px;">SC0319</td>
			<td>2x tubes for outflow, ID: 2.79 mm</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">CCD camera</td>
			<td style="width:244px;">PCO, Germany</td>
			<td style="width:179px;">pco.edge sCMOS</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">lens cleaning paper</td>
			<td style="width:244px;">Kodak</td>
			<td style="width:179px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">50 ml conical centrifuge tube</td>
			<td style="width:244px;">Thermo scientific</td>
			<td style="width:179px;">339652</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Prechamber</td>
			<td style="width:244px;">Warner Instruments</td>
			<td style="width:179px;">BSC-PC</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Inline heater</td>
			<td style="width:244px;">Warner Instruments</td>
			<td style="width:179px;">SF-28</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:311px;height:21px;">Temperature Controller</td>
			<td style="width:244px;">Warner Instruments</td>
			<td style="width:179px;">TC-324B</td>
			<td></td>
		</tr>
	</tbody>
</table>

recording synaptic plasticity in acute hippocampal slices maintained in a small-volume recycling-, perfusion-, and submersion-type chamber system

비록 뇌 조각에 실험 1951 년부터 사용 되었습니다, 문제는 필드 잠재적인 또는 세포내 녹음을 수행할 때 시 냅 시스 전송의 조음의 안정적이 고 성공적인 분석을 달성의 가능성을 감소 시키는 남아 있다. 이 원고는 상용 침수 챔버에 필드 흥분 성의 postsynaptic 잠재력 기록에 대 한 급성 뇌 조각의 유지 보수에 대 한 실험 조건 개선에 도움이 될 수 있는 방법론 기능을 설명 합니다. 와 유출 carbogenation 단위. 유출 carbogenation 약물 실험의 비용 효율성을 향상 시키기 위해 작은 버퍼 저수지의 재활용에 의존 하는 실험에서 산소 수준 안정화에 도움이 됩니다. 또한, 원고는 시 냅 시스 전송의 활동-종속 시 냅 스가 소성에 다른 carbogenation 모드와 자극 패러다임의 효과 검사 하는 대표적인 실험을 선물 한다.

프로토콜 섹션에서 우리는 조각의 급성 hippocampal hippocampal 형성 (그림 1)의 복 부와 중간 부분에서 남성 C57BL/6 마우스 및 남성 Wistar 쥐 (5-8 주)의 준비를 설명. 슬라이서 플랫폼에 반구의 위치 그들을 안정적으로 유지 하는 데 도움이 하 고 안정화 한 천 또는 agarose의 필요를 제거 합니다. 관류 시스템 자체 운영 하는 액체의 필요한 유량을 주고 높은 회?...

Watch this Scientific Journal Video about Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System at JoVE.com

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

급성 Hippocampal 조각에 작은 볼륨 재활용-, 관류-침수 형 챔버 시스템 유지에 시 냅 스가 소성을 기록

이 프로토콜 산소 수준 재활용된 버퍼의 작은 볼륨과 침수 급성 hippocampal 조각 활동 종속 시 냅 스가 소성 녹음의 방법 론 적 측면에서의 안정화를 설명 합니다.

Even though experiments on brain slices have been in use since 1951, problems remain that reduce the probability of achieving a stable and successful ...

Research

JoVE Journal

Neuroscience

Preparation of Oligomeric &beta;-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Preparation of Oligomeric ÃƒÅ½Ã‚Â²-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

β - 아밀로이드 Oligomeric의 준비 1-42</subHippocampal 조각에 시냅틱 소성 장애의>와 유도

Impairment of synaptic connections is likely to underlie the subtle amnesic changes occurring at the early stages of Alzheimer s Disease (AD). ...

연구

신경과학

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

노화와 아밀로이드 병리 동안 시냅틱 얼터 레이션의 연구에 대한 고양이와 트렌스 제닉 마우스의 급성 Hippocampal 조각의 준비

The rodent hippocampal slice preparation is perhaps the most broadly used tool for investigating mammalian synaptic function and plasticity. The ...

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

교양 뉴런의 막 단백질의 측면 보급과 Exocytosis은 형광 복구 및 형광 손실 Photobleaching를 사용하여 평가

Membrane proteins such as receptors and ion channels undergo active trafficking in neurons, which are highly polarised and morphologically complex. This ...

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

급성 Hippocampal 슬라이스에 Synaptically-evoked Astroglial와 Neuronal 응답의 듀얼 Electrophysiological 레코딩

Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs ...

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

해마의 Organotypic 조각의 쌍으로 전체 셀 녹음

Pair recordings involve simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling not only direct ...

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

설치류에서 급성 해마 조각을 사용하여 시냅스 태그 / 캡처 및 크로스 캡처 조사

Synaptic tagging and capture (STC) and cross-tagging are two important mechanisms at cellular level that explain how synapse-specificity and associativity ...

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

교양된 Hippocampal 신경에 시 냅 스 소포 Endocytosis를 측정

During endocytosis, fused synaptic vesicles are retrieved at nerve terminals, allowing for vesicle recycling and thus the maintenance of synaptic ...

Pentylenetetrazole-Induced Kindling Mouse Model

Pentylenetetrazole 유도 점화 마우스 모델

Pentylenetetrazole (PTZ) is a GABA-A receptor antagonist. An intraperitoneal injection of PTZ into an animal induces an acute, severe seizure at a high ...

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

급성 척수 조각 Substantia Gelatinosa 신경에 있는 전체 셀 패치 클램프 기록에 대 한 준비

Recent whole-cell patch-clamp studies from substantia gelatinosa (SG) neurons have provided a large body of information about the spinal mechanisms ...