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DOI :
10.3791/55938-v
April 12th, 2018
Chapters
0:04
Title
0:42
Stereotaxic Injection of Tracer
2:54
Perfusion and Sectioning
4:15
Double Fluorescent Staining for BDA and Basic Neural Elements in Cerebral Cortex
5:07
Results: Injection Site and Typical Neural Labeling with High Molecular Weight BDA in the Somatosensory Cortex
6:04
Conclusion
在这里, 我们提出了一个完善的协议, 以有效地揭示生物素化葡聚糖胺 (BDA) 标签的荧光染色方法通过一个相互的神经通路。它适用于分析 BDA 标签的精细结构, 并在共焦激光扫描显微镜下将其与其他神经元进行区分。
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