Name: fluorescence-mediated tomography for the detection and quantification of macrophage-related murine intestinal inflammation
Uploaded: 2017-12-15T12:30:00
Description: Watch this Scientific Journal Video about Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation at JoVE.com

우리는 우수한 기술 지원에 대 한 양 소냐 Dufentester, 양 Elke 웨버와 부인 Klaudia Niepagenkämper 감사합니다.

모든 동물 실험 Landesamt 위한 Natur, Umwelt und Verbraucherschutz (LANUV) 노르 트 베스트 팔 렌 독일 동물 보호 법률 (Tierschutzgesetz)에 의해 승인 되었다.
1. 재료 및 실험 설치
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	<li>동물 케어입니다.
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		<li>(예를 들어, C57BL/6) 어떤 DSS 취약 긴장의 성별 및 나이 일치 마우스를 사용 하 여 20 ~ 25 g bodyweight에.</li>
		<li>현지 동물 보호 지침에 따라 마우스 실험 그룹과 집...

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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small+animal+imaging.+current+technology+and+perspectives+for+oncological+imaging.">Small animal imaging. current technology and perspectives for oncological imaging.</a> Eur J Cancer. 38 (16), 2173-2188 (2002).</li><li>Bettenworth, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translational+18F-FDG+PET/CT+imaging+to+monitor+lesion+activity+in+intestinal+inflammation.">Translational 18F-FDG PET/CT imaging to monitor lesion activity in intestinal inflammation.</a> J Nucl Med. 54 (5), 748-755 (2013).</li><li>Vowinkel, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Apolipoprotein+A-IV+inhibits+experimental+colitis.">Apolipoprotein A-IV inhibits experimental colitis.</a> J Clin Invest. 114 (2), 260-269 (2004).</li><li>Korlach, J., Schwille, P., Webb, W. 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의료 이미징 기술을 최근 몇 년 동안에서 급속 하 게 진화, 비록 우리는 여전히 개발의 그들의 초기 단계에서 염증 성 프로세스 또는 종양, 뿐만 아니라 다른 질병을 감지 하는 우리의 능력에 제한 됩니다. 그러나, 이것은 이해 종양 성장, 침략, 또는 전이 개발 및 염증 성 질환 및 퇴행 성, 심장 혈관과 면역 질병의 발달에서 세포 프로세스에 매우 중요. 전통적인 이미징 기술을 신체적 또는 생리 적 ...

동물 모델 연구에서 널리 이용 된다 그리고 모니터 질병 활동과 활력, 몸 무게 변경의 정량화 등 혈액, 소변, 배설물의 분석에 많은 비-침략 적 절차 존재 한다. 그러나, 이들은 또한 간 개별 다양성에 적용 되는 간접 대리 매개 변수만 있습니다. 그들은 자주 조직 표본, 반복 시간 지점에서 직렬 관찰 방지의 사후 분석으로 보완 해야 한다 고 에 비보를 처리 하는 생리 적 또는 병 적인 관찰. 정교한 이미징 기술에 작은 동물 등장, 교차 단면 영상, 광학 이미징 및 내 시경, 이러한 프로세스의 직접적인 시각화를 가능 하 게 하 고 또한 같은 동물1 의 반복적인 분석에 대 한 허용을 포함 한 , 2 , 3. 또한, 반복적으로 같은 동물에 있는 질병의 다양 한 상태 모니터링에 동물 윤리의 관점에서 것 필요한 하는 동물의 수 저하 될 수 있습니다.
여러 다른 광학 이미징 기술을 형광 이미징 vivo에서 존재 한다. 원래, confocal 영상 표면과 표면 아래 형광 이벤트4,5공부를 고용 했다. 그러나 최근에,, 양적 3 차원 조직 평가 대 한 허용 하는 단층 촬영 시스템 개발된6되었습니다. 이 낮은 흡수, 민감한 탐지기와 단색 광원7근처-적외선 (NIR) 스펙트럼에서 빛을 방출 하는 형광 프로브 개발을 통해 성취 되었습니다. 전통적인 에칭한 이미징 기술을, 동안 같은 컴퓨터 단층 촬영 (CT), 자기 공명 영상 (MRI) 또는 초음파 (미국), 물리적에 주로 의존 하 고 시각화 형태학, 광학 이미징 제공할 수 있습니다 추가 정보 기본 분자 프로세스에8을프로브 생 또는 외 인 형광을 사용 합니다.
분자 생물학에서의 발전 목표의 증가 수에 대 한 스마트 하 고 타겟 형광 분자 프로브의 생성을 촉진 하기 위하여 왔다. 예를 들어 수용 체-중재 통풍 관 및 지정된 대상 지역에 배포 수 구상 될 carbocyanine 파생 표시 된 항 체9를 사용 하 여. 신체의 그렇지 않으면 액세스할 수 없는 영역에 특정 추적기 기능을 붙일 수는 사용 가능한 항 체의 풍부한 tumorigenesis, 신경의 모델에서 분자 및 세포 프로세스에 전례 없는 통찰력을 제공 심혈 관, 면 역학적, 그리고 염증 성 질병7.
이 연구에서 우리는 murine 모델 colitis의 단층 형광 중재를 사용 하 여를 설명합니다. Dextran 황산 나트륨 (DSS)-유도 대 장 염은 선 동적인 장 질병 (IBD)10유사한 창 자 염증의 화학적으로 유도 된 마우스 표준형. 특히 창 자 염증11의 개발에 타고 난 면역 체계의 기여를 평가 하기 위해 유용 합니다. 모집, 활성화, 및 monocytes 그리고 대 식 세포의 침투 IBD의 병 인에 중요 한 단계를 나타냅니다, 이후 그들의 신규 모집의 시각화 및 침투의 활동 모니터링, 예를 들어의 효과에 필수적입니다. 전 임상 설정12에 잠재적인 치료 물질. 우리는 DSS colitis의 유도 설명 하 고 monocyte/대 식 세포 마커 F4/80의 특정 시각화에 대 한 형광 분자 단층 촬영을 사용 하 여 창 자 점 막으로 대 식 세포 침투의 단층 촬영-중재 특성을 보여 13. 또한, 우리는 항 체 라벨; 등 보조 및 보완 절차를 설명 실험적인 체제; 그리고 분석 및 질병 활동 지 수 등 기존의 판독 상관 관계에서 얻은 이미지의 해석을 cytometry 및 조직학 분석과 immunohistochemistry 흐름. 우리는이 기술과 다른 이미지 형식에 대 한 비교의 한계를 논의.

<table border="0" cellpadding="0" cellspacing="0" width="1601">
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			<td height="21" style="height:21px;width:469px;">Reagents</td>
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			<td height="20" style="height:20px;">Leica DMLB Fluorescent Microscope</td>
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			<td>DMLB</td>
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			<td height="20" style="height:20px;width:469px;">Disposable scalpel No 10</td>
			<td>Sigma-Aldrich, Deisenhoffen, Germany</td>
			<td>Z692395-10EA</td>
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			<td height="20" style="height:20px;width:469px;">Metzenbaum scissors 14cm</td>
			<td>Ehrhardt Medizinprodukte GmbH, Geislingen, Germany</td>
			<td>22398330</td>
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			<td height="20" style="height:20px;">luer lock syringe 5ml</td>
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			<td style="width:320px;">Z248010</td>
			<td></td>
		</tr>
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			<td height="20" style="height:20px;width:469px;">syringe needles</td>
			<td>Sigma-Aldrich, Deisenhoffen, Germany</td>
			<td>Z192368&#160;</td>
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		<tr height="20">
			<td height="20" style="height:20px;">Falcon Tube 50ml</td>
			<td>BD Biosciences, Erembodegem, Belgium</td>
			<td style="width:320px;">352070</td>
			<td></td>
		</tr>
	</tbody>
</table>

fluorescence-mediated tomography for the detection and quantification of macrophage-related murine intestinal inflammation

질병의 murine 모델은 과학적 연구에 불가결. 그러나, 내 시경 검사 나 컴퓨터 단층 촬영 영상 등 많은 진단 도구는 정기적으로 동물 모델에서 채택 하지. 기존의 실험 판독 자주 내 개별 후속 시험을 방지 하 고 필요한 연구 동물의 수를 증가 하는 사후 고 비보 전 분석에 의존 합니다. 형광-중재 단층 형광 프로브의 비-침략 적, 반복, 양적, 3 차원 평가 수 있습니다. 매우 중요 한 특정 검색 및 고유한 분자 대상의 특성에 대 한 수 있는 분자 제조 업체의 사용 허가 하 고. 특히, 타겟된 프로브 염증, 자가 면역 질환, 감염, 혈관 질환, 셀 마이그레이션, tumorigenesis, 등에서 유전자 활성화 및 단백질 발현을 분석 하기 위한 혁신적인 도구를 나타냅니다. 이 문서에서는, 우리 단계별 지침을 제공이 정교한 이미징 기술에 vivo에서 탐지 및 (즉, f 4/80-긍정적인 대 식 세포 침투)의 널리 murine 모델에서 염증의 특성에 대 한 장 염증입니다. 이 기술은 또한 면역 세포 또는 줄기 세포 추적 같은 다른 연구 분야에서 사용할 수 있습니다.

Colitis의 평가:
DSS 유도 colitis 인간의 IBD를 닮 고 체중 감소, 직장 출혈, 표면 궤, 그리고 취약 쥐15점 막 손상에 이르게 하는 창 자 염증의 화학적으로 유도 된 murine 모델입니다. 타고 난 면역 체계가 장내 염증10,11의 발전에의 기여를 연구 특히 유용 합니?...

Watch this Scientific Journal Video about Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation at JoVE.com

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특정 대상 프로브 질병 (예를 들어, 염증, 감염, 및 tumorigenesis)의 다양 한 종류의 단백질 표정 등의 분자 메커니즘을 분석 하기 위한 혁신적인 도구를 나타냅니다. 이 연구에서 우리는 murine 모델 f 4/80 전용 형광 중재 단층 촬영을 사용 하 여 대 장 염의 장 대 식 세포 침투의 양적 차원 단층 평가 설명 합니다.

검색 및 대 식 세포 관련 Murine 창 자 염증의 정량화에 대 한 단층 형광 중재

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Atherosclerotic 플라크 현황 및 혈관 염증의 부량 사용하여 [을 18-F] Fluorodeoxyglucose 양전자 방출 Tomography / 계산된 Tomography (FDG-PET/CT)

Conventional non-invasive imaging modalities of atherosclerosis such as coronary artery calcium (CAC)1 and carotid intimal medial thickness (C-IMT)2 ...

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Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

에 대한 쥐의 내시경<em&gt; 생체</em&gt; 발암 및 소장의 평가의 멀티 모달 이미징 상처 치유 및 염증

Mouse models are widely used to study pathogenesis of human diseases and to evaluate diagnostic procedures as well as therapeutic interventions ...

Mouse Kidney Transplantation: Models of Allograft Rejection

마우스 신장 이식 : 동종 이식 거부의 모델

Rejection of the transplanted kidney in humans is still a major cause of morbidity and mortality. The mouse model of renal transplantation closely ...

A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction

돌이킬 수 및 가역 일방적 Ureteric 폐색의 쥐 모델

Obstruction of the kidney may affect native or transplanted kidneys and results in kidney injury and scarring. Presented here is a model of obstructive ...

Murine Model of Intestinal Ischemia-reperfusion Injury

창자 허혈 - 재관류 손상의 쥐 모델

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

대동맥 석회화와 염증의 혈관 평활근 세포 및 이미지의 석회화

Cardiovascular disease is the leading cause of morbidity and mortality in the world.  Atherosclerotic plaques, consisting of lipid-laden macrophages and ...