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DOI :
10.3791/55992-v
September 8th, 2017
Chapters
0:05
Title
0:49
Plating Cells for Vascular Cell co-culture
4:14
Harvesting Fractions from the VCCC
7:21
Harvesting and Embedding the VCCC for Transverse Immunofluorescence
9:11
Results: Myoendothelial Junction Formation in Vascular Cell Co-cultures
10:18
Conclusion
一个细胞培养模型的阻力动脉描述, 允许解剖的信号通路的内皮, 平滑肌, 或之间的内皮和平滑肌 (myoendothelial 交界处)。选择性应用激动剂或蛋白质隔离, 电子显微镜, 或免疫荧光可以利用这种细胞培养模型。
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