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DOI :
10.3791/55996-v
August 10th, 2017
Chapters
0:05
Title
0:35
Cell Seeding
2:00
Cell Transfection and Nocodazole Treatment
2:50
Image Acquisition of Multi-color Beads and Golgi Mini-stacks
4:36
Image Analysis, Acquire Centers of Fluorescent Beads
7:57
Select and Measure Golgi Mini-stacks
10:59
Results: Imaging the Golgi's Center of Fluorescence Mass
12:31
Conclusion
ゴルジの住民の正確な局在はゴルジ体の細胞の機能を理解するために不可欠です。しかし、従来の光学顕微鏡ではサブ ゴルジ構造を解決できません。ここで定量的蛋白質のサブ ゴルジ装置の局在を決定する従来の顕微鏡による超解像法のプロトコルについて述べる。
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