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DOI :
10.3791/55999-v
November 12th, 2017
Chapters
0:05
Title
0:44
Cultivation of HeLa-derived Cell Lines
1:48
Cell Transfection
3:21
Induction of Local DNA Lesions and Confocal Microscopy
4:47
Fluorescence Lifetime Image Microscopy
6:24
FRET Efficiency Using the FLIM-FRET Script
7:48
FRAP Analysis
8:50
Results: Analysis of Recruitment of mCherry-tagged PCNA and -53BP1 at DNA Lesions in HeLa Cells
11:00
Conclusion
激光 microirradiation 是研究活体细胞 DNA 修复的有效工具。一个方法的方法, 使用 UVA 激光诱导各种 DNA 病变显示。我们对维持正常细胞周期的局部 microirradiation 的方法进行了优化;因此, 辐照细胞通过有丝分裂进行。
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