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DOI :
10.3791/56001-v
July 10th, 2017
Chapters
0:05
Title
0:46
Genomic DNA Digestion
1:39
Genomic DNA Gel Electrophoresis
2:56
Gel Fluorescent Imaging and Hybridization
5:17
Hybridization with Telomere Probe
7:36
Gel Washing, Phospher Screen Exposure, and Radio Imaging
9:08
Results: Telomere Length is Quantified by a Modefied Terminal Restriction Fragment Analysis
10:28
Conclusion
在本文中,讨论了使用修饰的末端限制性片段分析量化端粒长度的详细方案,其提供了端粒长度的快速和有效的直接测量。该技术可以应用于DNA的各种细胞来源以量化端粒长度。
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