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DOI :
10.3791/56001-v
July 10th, 2017
Chapters
0:05
Title
0:46
Genomic DNA Digestion
1:39
Genomic DNA Gel Electrophoresis
2:56
Gel Fluorescent Imaging and Hybridization
5:17
Hybridization with Telomere Probe
7:36
Gel Washing, Phospher Screen Exposure, and Radio Imaging
9:08
Results: Telomere Length is Quantified by a Modefied Terminal Restriction Fragment Analysis
10:28
Conclusion
この記事では、テロメアの長さを迅速かつ効率的に直接測定できる改良されたターミナル制限断片解析を使用してテロメアの長さを定量化するための詳細なプロトコルについて説明します。この技術は、テロメアの長さを定量するためのDNAの様々な細胞源に適用することができる。
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