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DOI :
10.3791/56002-v
September 6th, 2017
Chapters
0:05
Title
1:22
Isolation of Hematopoietic Bone Marrow cells by Multi-parameter Fluorescence-Actviated Cell Sorting (FACS)
6:33
Clonogenic Assay
7:54
Analysis of Chimerism by PCR
9:16
Results: Quantifying Relative Proportions of Donor-versus-recipient Chimerism in the Erythroid Progenitors
10:54
Conclusion
ドナー由来細胞の定量は、赤血球の溶血患者の幹細胞移植後の生着を監視する必要です。流れフローサイトメトリーを用いた細胞の選別、コロニー形成アッセイと短いタンデム繰り返しは増殖を評価するために使用可能性がありますの後の分析と赤芽球のコンパートメントの前駆細胞の分化の組み合わせ。
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