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DOI :
10.3791/56011-v
July 28th, 2017
Chapters
0:05
Title
1:15
In Vivo mRNA-protein Crosslinking by UV Irradiation
2:38
Protoplast Lysis Under Denaturing Conditions
3:59
mRNP Pull-down and Purification by Oligo-d(T)25 Beads
8:14
mRBP Concentration and Protein Assays
10:04
Results: Capture of UV-crosslinked mRNA-bound Proteins by Oligo-d(T)25 Beads
11:53
Conclusion
여기, 우리는 Arabidopsis thaliana 엽초 엽 원형체에 적용된 상호 작용 캡처 프로토콜을 제시한다. 이 방법은 생체 내 UV 가교 결합에 비판적으로 의존하며 생리 환경으로부터 식물 mRNA 결합 단백질의 분리 및 동정을 허용합니다.
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