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DOI :
10.3791/56061-v
•
13:49 min
December 6th, 2017
Chapters
0:05
Title
1:00
Negative vs Positive Sorting
1:51
AutoMACS Bacterial Display Library Biopanning Positive Sorting
6:27
FACS Analysis of Sorting Rounds
11:04
Results: Representative Peptide Affinity and Specificity Date, and Alignment and Consensus Determination for Sequencing Results
12:46
Conclusion
筛选细菌显示库是一种被证实的技术, 用于发现肽亲和试剂, 一种强有力的替代抗体。本文的半自动排序方法简化了筛选, 减少了误报的发生。在这里, 我们说明的思想过程和技术应用于评价候选人和最小化下游分析。
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