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DOI :
10.3791/56061-v
•
13:49 min
December 6th, 2017
Chapters
0:05
Title
1:00
Negative vs Positive Sorting
1:51
AutoMACS Bacterial Display Library Biopanning Positive Sorting
6:27
FACS Analysis of Sorting Rounds
11:04
Results: Representative Peptide Affinity and Specificity Date, and Alignment and Consensus Determination for Sequencing Results
12:46
Conclusion
バイオパニング細菌の表示ライブラリは、抗体に堅牢な代替ペプチド親和性試薬の発見の実績のある手法です。半自動ソーティング法を本書は誤検知の発生を減らすバイオパニングを合理化します。ここでは、思考プロセスと候補者を評価し、下流の解析を最小限に適用される技術を示しています。
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