Dorsolateral Telencephalon Microdissection and Cell Plating
4:23
Time-lapse Video Microscopy
5:24
Cell Tracking
8:12
Results: Cell Lineage of Single Progenitor Cells
9:41
Conclusion
Transcript
The overall goal of this experiment is to observe cell behaviors during lineage progression from neural stem cells to neurons or glial cells. This method can help answering questions in the neurodevelopmental field such as the role of symmetric an
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Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.