Name: assessment of human adipose tissue microvascular function using videomicroscopy
Uploaded: 2017-09-29T13:00:00
Description: Watch this Scientific Journal Video about Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy at JoVE.com

作者希望感谢志愿者参与这些研究, 以及波士顿医疗中心的外科工作人员提供脂肪组织活检。Dr. Gokce 由国立卫生研究院 (NIH) 资助 HL081587、HL114675 和 HL126141。Dr. Farb 由 NIH 资助 K23 HL135394。

此处所述的协议和示例是由波士顿大学医学院机构审查委员会 (IRB, 议定书 #H-25644) 批准的, 并按照《赫尔辛基宣言》进行。所有受试者在参与前均提供书面知情同意. 
 1. 溶液的制备和微玻璃套管 <ol> <li> 准备 4-2-hydrosyethyl-1-piperazineethanesulfonic 酸性盐水 (HEPES) 溶液。1升, 溶解8.059 克氯化钠, 0.298 克氯化钾, 0.296 g MgSO 4 和 #8226; 7H 2 O、0.235 g CaCl 2 和 #8...

<ol>
	<li>Schubert, R., Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+myogenic+response:+established+facts+and+attractive+hypotheses.">The myogenic response: established facts and attractive hypotheses.</a> Clin Sci (Lond). 96 (4), 313-326 (1999).</li><li>Jadeja, R. N., Rachakonda, V., Bagi, Z., Khurana, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessing+Myogenic+Response+and+Vasoactivity+In+Resistance+Mesenteric+Arteries+Using+Pressure+Myography.">Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography.</a> J Vis Exp. (101), e50997 (2015).</li><li>Gutterman, D. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Human+Microcirculation:+Regulation+of+Flow+and+Beyond.">The Human Microcirculation: Regulation of Flow and Beyond.</a> Circ Res. 118 (1), 157-172 (2016).</li><li>Fuster, J. J., Ouchi, N., Gokce, N., Walsh, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Obesity-Induced+Changes+in+Adipose+Tissue+Microenvironment+and+Their+Impact+on+Cardiovascular+Disease.">Obesity-Induced Changes in Adipose Tissue Microenvironment and Their Impact on Cardiovascular Disease.</a> Circ Res. 118 (11), 1786-1807 (2016).</li><li>Farb, M. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+adipose+tissue+inflammation+represents+an+intermediate+cardiometabolic+phenotype+in+obesity.">Reduced adipose tissue inflammation represents an intermediate cardiometabolic phenotype in obesity.</a> J Am Coll Cardiol. 58 (3), 232-237 (2011).</li><li>Farb, M. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=WNT5A-JNK+regulation+of+vascular+insulin+resistance+in+human+obesity.">WNT5A-JNK regulation of vascular insulin resistance in human obesity.</a> Vasc Med. 21 (6), 489-496 (2016).</li><li>Durand, M. J., Phillips, S. A., Widlansky, M. E., Otterson, M. F., Gutterman, D. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+vascular+renin-angiotensin+system+contributes+to+blunted+vasodilation+induced+by+transient+high+pressure+in+human+adipose+microvessels.">The vascular renin-angiotensin system contributes to blunted vasodilation induced by transient high pressure in human adipose microvessels.</a> Am J Physiol Heart Circ Physiol. 307 (1), H25-H32 (2014).</li><li>Farb, M. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cyclooxygenase+inhibition+improves+endothelial+vasomotor+dysfunction+of+visceral+adipose+arterioles+in+human+obesity.">Cyclooxygenase inhibition improves endothelial vasomotor dysfunction of visceral adipose arterioles in human obesity.</a> Obesity (Silver Spring). 22 (2), 349-355 (2014).</li><li>Park, S. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+age+on+the+vasodilatory+function+of+human+skeletal+muscle+feed+arteries.">Impact of age on the vasodilatory function of human skeletal muscle feed arteries.</a> Am J Physiol Heart Circ Physiol. 310 (2), H217-H225 (2016).</li><li>Ives, S. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+skeletal+muscle+feed+arteries+studied+in+vitro:+the+effect+of+temperature+on+alpha(1)-adrenergic+responsiveness.">Human skeletal muscle feed arteries studied in vitro: the effect of temperature on alpha(1)-adrenergic responsiveness.</a> Exp Physiol. 96 (9), 907-918 (2011).</li><li>Grizelj, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+flow-and+acetylcholine-induced+dilations+in+visceral+compared+to+subcutaneous+adipose+arterioles+in+human+morbid+obesity.">Reduced flow-and acetylcholine-induced dilations in visceral compared to subcutaneous adipose arterioles in human morbid obesity.</a> Microcirculation. 22 (1), 44-53 (2015).</li><li>Farb, M. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Arteriolar+function+in+visceral+adipose+tissue+is+impaired+in+human+obesity.">Arteriolar function in visceral adipose tissue is impaired in human obesity.</a> Arterioscler Thromb Vasc Biol. 32 (2), 467-473 (2012).</li><li>Tanner, M. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dynamin-Related+Protein+1+Mediates+Low+Glucose-Induced+Endothelial+Dysfunction+in+Human+Arterioles.">Dynamin-Related Protein 1 Mediates Low Glucose-Induced Endothelial Dysfunction in Human Arterioles.</a> Am J Physiol Heart Circ Physiol. , (2016).</li><li>Ives, S. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=alpha1-Adrenergic+responsiveness+in+human+skeletal+muscle+feed+arteries:+the+impact+of+reducing+extracellular+pH.">alpha1-Adrenergic responsiveness in human skeletal muscle feed arteries: the impact of reducing extracellular pH.</a> Exp Physiol. 98 (1), 256-267 (2013).</li><li>Dharmashankar, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nitric+oxide+synthase-dependent+vasodilation+of+human+subcutaneous+arterioles+correlates+with+noninvasive+measurements+of+endothelial+function.">Nitric oxide synthase-dependent vasodilation of human subcutaneous arterioles correlates with noninvasive measurements of endothelial function.</a> Am J Hypertens. 25 (5), 528-534 (2012).</li><li>Truran, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+and+leptomeningeal+arteriole+endothelial+dysfunction+induced+by+beta-amyloid+peptide:+a+practical+human+model+to+study+Alzheimer's+disease+vasculopathy.">Adipose and leptomeningeal arteriole endothelial dysfunction induced by beta-amyloid peptide: a practical human model to study Alzheimer's disease vasculopathy.</a> J Neurosci Methods. 235, 123-129 (2014).</li><li>Karki, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Forkhead+box+O-1+modulation+improves+endothelial+insulin+resistance+in+human+obesity.">Forkhead box O-1 modulation improves endothelial insulin resistance in human obesity.</a> Arterioscler Thromb Vasc Biol. 35 (6), 1498-1506 (2015).</li><li>Shahid, M., Buys, E. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessing+murine+resistance+artery+function+using+pressure+myography.">Assessing murine resistance artery function using pressure myography.</a> J Vis Exp. (76), (2013).</li></ol>

从周围组织中剥离和分离脂肪动脉可能是一个费时和 labor-intensive 的过程, 并仔细注意细节和技术协议。该显微解剖程序需要细致的技能和专门的解剖用具, 以防止对平滑肌或血管内皮细胞层的潜在损害。即使在动脉壁微小的意外穿刺也能防止腔内加压, 并导致不成功的实验。此外, 利用缝线的小动脉插管对确保血管末端的玻璃毛细管吸管尖端之间的微动脉是至关重要的。
人孤?...

Videomicroscopy 是一种有用的技术, 用于检查从活着的人的主体前体去除小动脉的血管功能。我们的实验室专注于解剖从不同的脂肪组织隔间微小的微血管, 以表征各种脂肪环境的影响。这项技术的一个主要优点是, 从人体中取出的血管仍然是功能性的, 在活检后几分钟到几小时内就可以很容易地检查。通过微玻璃套管在腔内间隙中模拟和稳定的壁压力, 从而重现许多体内特征。1,2此外, 使用自动边缘检测软件进行可靠的 videomicroscopy 设置, 可以对内皮依赖性和独立的血管扩张剂和收缩的分离能力进行定性和定量评估。血管在 real-time, 允许快速的生理评估反应的物理和药理刺激。3其他微血管技术也可以使用, 如导线 myography, 它的耗时更少, 并通过力传感器测量各种激动剂的张力反应。
我们的实验室已经应用 videomicroscopy 来研究肥胖和血管功能紊乱之间的关系, 重点研究不同脂肪组织领域对脉管的影响。中央肥胖与腹内内脏脂肪的积累一直是最密切联系的因子生产, 代谢功能紊乱, 和代谢风险。据推测, 脂肪组织功能障碍与生产过剩的 pro-atherogenic 细胞因子和脂肪失调是强烈牵连在这些过程中, 但具体的调控分子和治疗指标仍然很大程度上发现.4此外, 在局部脂肪组织水平, 毛细血管稀疏和受损灌注已与脂肪组织 pseudohypoxia 和代谢失调。心血管疾病可能是脂肪组织功能紊乱的结果的假说正在演变。从脂肪释放的亲动脉粥样硬化调解人, 特别是与中央/内脏肥胖的联系, 可能促进内皮功能障碍和致病血管的变化, 可以在当地的脂肪血管显着, 并检测使用本文介绍了方法学。5
孤立性脂肪组织动脉的功能评估是一个有用的方法, 以获得理解的病理生理学和分子机制, 导致血管功能障碍的人肥胖。为了研究有助于脂肪库特异性功能障碍的机制, 我们已经制定了检查血管内皮依赖性和独立血管反应的方法, 并评价了各种调节在减肥手术时从肥胖患者身上获得的内脏和皮下 (SC) 脂肪组织标本的候选者。

<table border="0" cellpadding="0" cellspacing="0" width="769">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:352px;">Chemical Name</td>
			<td style="width:159px;"></td>
			<td style="width:132px;"></td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:352px;">Acetylcholine</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">A6625</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;width:352px;">Calcium chloride (CaCl2)</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td>223506</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:352px;">D-(+)-Glucose</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">G5767</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:352px;">Endothelin-1</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">E7764</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:352px;">Ethylene glycol-bis(2-aminoethylether)-N,N,N&#8217;,N&#8217;-tetra acetic acid (EGTA)</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">E3889</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:352px;">Ethylene diamine tetra acetic acid (EDTA)</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">E9884</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:352px;">HEPES</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">H3784</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="46">
			<td height="46" style="height:46px;width:352px;">Nw-nito-L-arginine methyl ester hydrochloride</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">N5751</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;width:352px;">Magnesium sulfate (MgSO4)</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">M7506</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:352px;">Potassium chloride (KCL)</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">P3911</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;width:352px;">Potassium phosphate (KH2PO4)</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">P5655</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:352px;">Papaverine</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">P3510</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;width:352px;">Sodium bicarbonate (NaHCO3 )</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">S6014</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:352px;">Sodium chloride (NaCl)</td>
			<td style="width:159px;">Sigma Aldrich</td>
			<td style="width:132px;">S7653</td>
			<td style="width:127px;"></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:352px;">Sodium phosphate monobasic monohydrate (NaH2Po4)</td>
			<td>Sigma Aldrich</td>
			<td>S9638</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Name</td>
			<td style="width:159px;">Company</td>
			<td style="width:132px;">Catalog Number</td>
			<td style="width:127px;">Comments</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Equipment</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Forceps</td>
			<td>Finescience tools</td>
			<td>15000-08</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Inverted microscope</td>
			<td>Zeiss Achromat</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Laboratory tubing</td>
			<td>Euro-Pharm</td>
			<td>250100306F999</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Needle/pippette puller</td>
			<td>David kopf instruments</td>
			<td>720</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Ophthalmic monofilament nylon suture</td>
			<td>Surgical specialties</td>
			<td>A7756N</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Scissors</td>
			<td>Finescience tools</td>
			<td>150000-08</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Vessel Chamber</td>
			<td>DMT</td>
			<td>VAS v.2.1</td>
			<td></td>
		</tr>
	</tbody>
</table>

assessment of human adipose tissue microvascular function using videomicroscopy

虽然肥胖与新陈代谢和心血管疾病的发展密切相关, 但对这些过程的机制却知之甚少。这是假设, 从脂肪组织释放的 pro-atherogenic 调解人, 特别是与中央/内脏肥胖的联系可能会促进病原体血管的变化, 在当地和系统, 并认为心血管疾病可能是脂肪组织功能障碍的后果继续发展。在这里, 我们描述了一个独特的 videomicroscopy 的方法, 包括分析的血管扩张和收缩反应的完整小动脉从活的人体脂肪库去除。Videomicroscopy 是用来检测孤立微血管的功能性质, 以响应药理或生理刺激使用的压力系统模拟在体内条件。该技术是一个有用的方法, 以获得理解的病理生理学和分子机制, 导致血管功能紊乱局部在脂肪组织环境。此外, 脂肪组织微血管的异常也与全身性疾病有关。我们运用这项技术来检查在肥胖人群中的特定于仓库的血管反应。我们评估了内皮依赖性血管舒张的增加流量和乙酰胆碱在脂肪动脉 (50-350 µm 内径, 2-3 毫米) 从两个不同的脂肪库从同一个人的减肥手术中分离出来。我们证明, 从内脏脂肪的动脉表现受损内皮依赖性血管舒张与从皮下仓库分离。研究结果表明, 内脏微环境与血管内皮功能障碍有关, 这可能与临床观察相关, 将内脏肥胖增加与系统性疾病机制联系起来。videomicroscopy 技术可用于检查不同脂肪库的血管表型, 并对不同程度肥胖和代谢功能障碍的个体进行比较研究。该方法还可用于对临床干预的反应纵向检查血管反应。

我们的实验室使用 videomicroscopy 检查内皮依赖性和独立血管舒张, 以及脂肪组织动脉的 vasocontractile 功能从皮下和内脏脂肪的肥胖的人。特性实验设置显示在图 1A中。脂肪组织动脉悬浮在两个玻璃毛细管管之间, 并固定在器官腔内的缝合处, 如图 1B所示。如上文5.1 节所述, 动脉的反应可以通过在基线 (图 2A) ?...

Watch this Scientific Journal Video about Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy at JoVE.com

Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy

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Videomicroscopy 系统用于检测孤立脂肪组织动脉的功能特性, 以响应生理和药理刺激。该技术可用于研究肥胖人群不同脂肪组织领域的微血管表型。

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Non-invasive Assessment of Microvascular and Endothelial Function

非侵入性的评估微血管和内皮功能

The authors have utilized capillaroscopy and forearm blood flow techniques to investigate the role of microvascular dysfunction in pathogenesis of ...

Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

小鼠Spinotrapezius模型来评估的影响小动脉结扎​​术，微血管功能与重塑

The murine spinotrapezius is a thin, superficial skeletal support muscle that extends from T3 to L4, and is easily accessible via dorsal skin incision. ...

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

在肿瘤血管生成中使用荧光共聚焦电视显微镜活体成像

Fibered confocal fluorescence in vivo imaging with a fiber optic bundle uses the same principle as fluorescent confocal microscopy. It can excite ...

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

肩胛间褐色脂肪组织的切伦科夫发光成像

Brown adipose tissue (BAT), widely known as a &#8220;good fat&#8221; plays pivotal roles for thermogenesis in mammals. This special tissue is closely ...

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

封装产热前脂肪细胞移植到脂肪组织仓库

Cell encapsulation was developed to entrap viable cells within semi-permeable membranes. The engrafted encapsulated cells can exchange low molecular ...

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

应用流式细胞仪对人体脂肪组织免疫细胞的表征

Infiltration of immune cells in the subcutaneous and visceral adipose tissue (AT) deposits leads to a low-grade inflammation contributing to the ...

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

18F-fdg Petct 对活性人棕色脂肪组织的全身和区域定量

In endothermic animals, brown adipose tissue (BAT) is activated to produce heat for defending body temperature in response to cold. BAT&#39;s ability to ...

A Computerized Functional Skills Assessment and Training Program Targeting Technology Based Everyday Functional Skills

以基于技术的日常功能技能为目标的计算机化功能技能评估和培训计划

Today, many functional skills are technology-based, so development of a technology-based training program has broad importance. Here we present a ...

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

使用皮肤心肌细胞进行心脏功能的体外评估

In this article, we describe the steps required to isolate a single permeabilized (&#34;skinned&#34;) cardiomyocyte and attach it to a force-measuring ...